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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1976 Aug;73(8):2654–2658. doi: 10.1073/pnas.73.8.2654

Small fragments from the A subunit of cholera toxin capable of activating adenylate cyclase.

Y Matuo, M A Wheeler, M W Bitensky
PMCID: PMC430706  PMID: 60760

Abstract

Exposure of cholera toxin to membrane particles prepared from sarcoma 180 cells gives rise to a variety of fragments which are capable of activating adenylate cyclase [ATP:pyrophosphate-lyase (cyclizing), EC 4.6.1.1]. A major component of these fragments has an apparent molecular weight in the 8,000-10,000 range. The smallest stimulatory fragment has a molecular weight of approximately 1400. The small size of the fragments is confirmed by Sephadex gel filtration, in the presence of either sodium dodecyl sulfate or formic acid. These fragments are produced from holotoxin or its A subunit by protease(s) found in sarcoma membrane particles. Production of fragments appears optimal in 40-60 min at 30 degrees and pH 7, and is prevented by protease inhibitors. The ability of the small fragments to activate adenylate cyclase is reversed by anti-holotoxin, but not anticholeragenoid, antibodies. These fragments require NAD for the activation of adenylate cyclase and are fully active after heating at 90 degrees for 5 min (pH 7).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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