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. 1976 Aug;73(8):2674–2678. doi: 10.1073/pnas.73.8.2674

Appearance in vivo of single-stranded complementary ends on parental herpesvirus DNA.

J H Jean, T Ben-Porat
PMCID: PMC430710  PMID: 183205

Abstract

Intracellular forms of pseudorabies virus parental DNA were examined before and after the onset of viral DNA synthesis. Before initiation of synthesis, parental viral DNA acquires single-stranded ends. Circular and concatemeric molecules are also observed, indicating that the single-stranded ends are complementary. Viral DNA replication is initiated at an internal site within the DNA molecule, giving rise to characteristic replicative loops with single-stranded regions in the trans position. Such replicative loops were seen in unit-size (and smaller than unit-size) linear molecules as well as in circular and concatemeric molecules. These results show that the parental viral DNA molecules that acquire single-stranded ends, and consequently are able to form circles and concatemers, proceed to replicate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEN-PORAT T., KAPLAN A. S. The synthesis and fate of pseudorabies virus DNA in infected mammalian cells in the stationary phase of growth. Virology. 1963 Jun;20:310–317. doi: 10.1016/0042-6822(63)90120-x. [DOI] [PubMed] [Google Scholar]
  2. Ben-Porat T., Kaplan A. S., Stehn B., Rubenstein A. S. Concatemeric forms of intracellular herpesvirus DNA. Virology. 1976 Feb;69(2):547–560. doi: 10.1016/0042-6822(76)90484-0. [DOI] [PubMed] [Google Scholar]
  3. Ben-Porat T., Stehn B., Kaplan A. S. Fate of parental herpesvirus DNA. Virology. 1976 Jun;71(2):412–422. doi: 10.1016/0042-6822(76)90369-x. [DOI] [PubMed] [Google Scholar]
  4. Biswal N., Murray B. K., Benyesh-Melnick M. Ribonucleotides in newly synthesized DNA of herpes simplex virus. Virology. 1974 Sep;61(1):87–99. doi: 10.1016/0042-6822(74)90244-x. [DOI] [PubMed] [Google Scholar]
  5. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  6. Frenkel N., Roizman B. Separation of the herpesvirus deoxyribonucleic acid duplex into unique fragments and intact strand on sedimentation in alkaline gradients. J Virol. 1972 Oct;10(4):565–572. doi: 10.1128/jvi.10.4.565-572.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grafstrom R. H., Alwine J. C., Steinhart W. L., Hill C. W. Terminal repetitions in herpes simplex virus type 1 DNA. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):679–681. doi: 10.1101/sqb.1974.039.01.081. [DOI] [PubMed] [Google Scholar]
  8. Huberman J. A. Visualization of replicating mammalian and T4 bacteriophage DNA. Cold Spring Harb Symp Quant Biol. 1968;33:509–524. doi: 10.1101/sqb.1968.033.01.059. [DOI] [PubMed] [Google Scholar]
  9. KAPLAN A. S., BEN-PORAT T. MODE OF REPLICATION OF PSEUDORABIES VIRUS DNA. Virology. 1964 May;23:90–95. doi: 10.1016/s0042-6822(64)80011-8. [DOI] [PubMed] [Google Scholar]
  10. KAPLAN A. S., VATTER A. E. A comparison of herpes simplex and pseudorabies viruses. Virology. 1959 Apr;7(4):394–407. doi: 10.1016/0042-6822(59)90068-6. [DOI] [PubMed] [Google Scholar]
  11. Kaschka-Dierich C., Adams A., Lindahl T., Bornkamm G. W., Bjursell G., Klein G., Giovanella B. C., Singh S. Intracellular forms of Epstein-Barr virus DNA in human tumour cells in vivo. Nature. 1976 Mar 25;260(5549):302–306. doi: 10.1038/260302a0. [DOI] [PubMed] [Google Scholar]
  12. Rubenstein A. S., Kaplan A. S. Electron microscopic studies of the DNA of defective and standard pseudorabies virions. Virology. 1975 Aug;66(2):385–392. doi: 10.1016/0042-6822(75)90211-1. [DOI] [PubMed] [Google Scholar]
  13. Sheldrick P., Berthelot N. Inverted repetitions in the chromosome of herpes simplex virus. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):667–678. doi: 10.1101/sqb.1974.039.01.080. [DOI] [PubMed] [Google Scholar]
  14. Shlomai J., Friedmann A., Becker Y. Replication intermediates of herpes simplex virus DNA. Virology. 1976 Feb;69(2):647–659. doi: 10.1016/0042-6822(76)90493-1. [DOI] [PubMed] [Google Scholar]
  15. Vogt V. M. Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae. Eur J Biochem. 1973 Feb 15;33(1):192–200. doi: 10.1111/j.1432-1033.1973.tb02669.x. [DOI] [PubMed] [Google Scholar]
  16. WARNER J. R., KNOPF P. M., RICH A. A multiple ribosomal structure in protein synthesis. Proc Natl Acad Sci U S A. 1963 Jan 15;49:122–129. doi: 10.1073/pnas.49.1.122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wadsworth S., Hayward G. S., Roizman B. Anatomy of herpes simplex virus DNA. V. Terminally repetitive sequences. J Virol. 1976 Feb;17(2):503–512. doi: 10.1128/jvi.17.2.503-512.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wadsworth S., Jacob R. J., Roizman B. Anatomy of herpes simplex virus DNA. II. Size, composition, and arrangement of inverted terminal repetitions. J Virol. 1975 Jun;15(6):1487–1497. doi: 10.1128/jvi.15.6.1487-1497.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wilkie N. M. The synthesis and substructure of herpesvirus DNA: the distribution of alkali-labile single strand interruptions in HSV-1 DNA. J Gen Virol. 1973 Dec;21(3):453–467. doi: 10.1099/0022-1317-21-3-453. [DOI] [PubMed] [Google Scholar]

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