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. 1977 Apr;74(4):1538–1542. doi: 10.1073/pnas.74.4.1538

Transmembrane movement of cholesterol in human erythrocytes.

Y Lange, C M Cohen, M J Poznansky
PMCID: PMC430825  PMID: 266194

Abstract

We studied the exchange of cholesterol between radioactively labeled plasma and human erythrocytes. Results from experiments in which [3H]cholesterol and [14C]-cholesterol were exchanged sequentially into the cells and back out into unlabeled plasma, showed that transmembrane movement of cholesterol occurred with a half-time that was either less than 50 min or greater than 10 days. To obtain further information about the transmembrane movement of cholesterol, we used a technique [Jacobson, B. S. & Branton, D. (1977) Science 195, 302-304] for exposure of the cytoplasmic surface of erythrocyte membranes. This method involved the ionic attachment of erythrocytes to derivatized glass beads followed by disruption of the cells, leaving the beads covered by membrane with the cytoplasmic surface exposed [3H]Cholesterol was exchanged into intact erythrocytes which then were attached to beads. The beads with attached membrane were incubated with phospholipid-cholesterol vesicles and the exchange of cholesterol between the membrane cytoplasmic surface and vesicles was studied. We found that [3H]cholesterol was present at the cytoplasmic surface, indicating that transmembrane movement of cholesterol had occurred within the 2.5 hr required to complete the experiment. This result suggests that the more rapid rate of transmembrane cholesterol movement, inferred from the experiments described above, is the one that applies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BASFORD J. M., GLOVER J., GREEN C. EXCHANGE OF CHOLESTEROL BETWEEN HUMAN BETA-LIPOPROTEINS AND ERYTHROCYTES. Biochim Biophys Acta. 1964 Dec 2;84:764–766. doi: 10.1016/0926-6542(64)90039-3. [DOI] [PubMed] [Google Scholar]
  2. Bell F. P., Schwartz C. J. Exchangeability of cholesterol between swine serum lipoproteins and erythrocytes, in vitro. Biochim Biophys Acta. 1971 May 4;231(3):553–557. doi: 10.1016/0005-2760(71)90126-3. [DOI] [PubMed] [Google Scholar]
  3. Bloj B., Zilversmit D. B. Asymmetry and transposition rates of phosphatidylcholine in rat erythrocyte ghosts. Biochemistry. 1976 Mar 23;15(6):1277–1283. doi: 10.1021/bi00651a017. [DOI] [PubMed] [Google Scholar]
  4. DODGE J. T., MITCHELL C., HANAHAN D. J. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch Biochem Biophys. 1963 Jan;100:119–130. doi: 10.1016/0003-9861(63)90042-0. [DOI] [PubMed] [Google Scholar]
  5. Fisher K. A. Analysis of membrane halves: cholesterol. Proc Natl Acad Sci U S A. 1976 Jan;73(1):173–177. doi: 10.1073/pnas.73.1.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HAGERMAN J. S., GOULD R. G. The in vitro interchange of cholesterol between plasma and red cells. Proc Soc Exp Biol Med. 1951 Oct;78(1):329–332. doi: 10.3181/00379727-78-19064. [DOI] [PubMed] [Google Scholar]
  7. Hollander d' F., Chevallier F. Movement of cholesterol in vitro in rat blood and quantitation of the exchange of free cholesterol between plasma and erythrocytes. J Lipid Res. 1972 Nov;13(6):733–744. [PubMed] [Google Scholar]
  8. Jacobson B. S., Branton D. Plasma membrane: rapid isolation and exposure of the cytoplasmic surface by use of positively charged beads. Science. 1977 Jan 21;195(4275):302–304. doi: 10.1126/science.831278. [DOI] [PubMed] [Google Scholar]
  9. Kahlenberg A., Walker C., Rohrlick R. Evidence for an asymmetric distribution of phospholipids in the human erythrocyte membrane. Can J Biochem. 1974 Sep;52(9):803–806. doi: 10.1139/o74-114. [DOI] [PubMed] [Google Scholar]
  10. MURPHY J. R. ERYTHROCYTE METABOLISM. VI. CELL SHAPE AND THE LOCATION OF CHOLESTEROL IN THE ERYTHROCYTE MEMBRANE. J Lab Clin Med. 1965 May;65:756–774. [PubMed] [Google Scholar]
  11. Poznansky M., Lange Y. Transbilayer movement of cholesterol in dipalmitoyllecithin-cholesterol vesicles. Nature. 1976 Feb 5;259(5542):420–421. doi: 10.1038/259420a0. [DOI] [PubMed] [Google Scholar]
  12. Quarfordt S. H., Hilderman H. L. Quantitation of the in vitro free cholesterol exchange of human red cells and lipoproteins. J Lipid Res. 1970 Nov;11(6):528–535. [PubMed] [Google Scholar]
  13. Renooij W., Van Golde L. M., Zwaal R. F., Van Deenen L. L. Topological asymmetry of phospholipid metabolism in rat erythrocyte membranes. Evidence for flip-flop of lecithin. Eur J Biochem. 1976 Jan 2;61(1):53–58. doi: 10.1111/j.1432-1033.1976.tb09996.x. [DOI] [PubMed] [Google Scholar]

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