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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1977 May;74(5):1942–1945. doi: 10.1073/pnas.74.5.1942

Primary structure of very low density apolipoprotein C-II of human plasma.

R L Jackson, H N Baker, E B Gilliam, A M Gotto Jr
PMCID: PMC431048  PMID: 194244

Abstract

Apolipoprotein C-II (apoC-II), a protein constituent of very low density lipoproteins of human plasma and the activator protein of lipoprotein lipase, has been isolated and its amino acid sequence has been studied. The protein has 78 amino acid residues and is lacking cysteine, cystine, and histidine. Chromatography on Bio-Gel P-30 in 25% formic acid of the cyanogen bromide digest of apoC-II yields three fragments designated as CNBr-I, -II, and -III. They contained 50, 19, and 9 residues, respectively. The alignment of the cyanogen bromide fragments has been established as CNBr-III-I-II by isolation and sequence of the tryptic peptides of the intact protein. The amino acid sequences of the tryptic and CNBr peptides were determined by conventional methods. With this information, it was possible to establish the complete amino acid sequence of apoC-II.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alaupovic P. Apoliproproteins and lipoproteins. Atherosclerosis. 1971 Mar-Apr;13(2):141–146. doi: 10.1016/0021-9150(71)90018-9. [DOI] [PubMed] [Google Scholar]
  2. Albers J. J., Scanu A. M. Isoelectric fractionation and characterization of polypeptides from human serum very low density lipoproteins. Biochim Biophys Acta. 1971 Apr 27;236(1):29–37. doi: 10.1016/0005-2795(71)90145-0. [DOI] [PubMed] [Google Scholar]
  3. Assmann G., Brewer H. B., Jr A molecular model of high density lipoproteins. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1534–1538. doi: 10.1073/pnas.71.4.1534. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baker H. N., Delahunty T., Gotto A. M., Jr, Jackson R. L. The primary structure of high density apolipoprotein-glutamine-I. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3631–3634. doi: 10.1073/pnas.71.9.3631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Baker H. N., Gotto A. M., Jr, Jackson R. L. The primary structure of human plasma high density apolipoprotein glutamine I (ApoA-I). II. The amino acid sequence and alignment of cyanogen bromide fragments IV, III, and I. J Biol Chem. 1975 Apr 10;250(7):2725–2738. [PubMed] [Google Scholar]
  6. Brewer H. B., Jr, Lux S. E., Ronan R., John K. M. Amino acid sequence of human apoLp-Gln-II (apoA-II), an apolipoprotein isolated from the high-density lipoprotein complex. Proc Natl Acad Sci U S A. 1972 May;69(5):1304–1308. doi: 10.1073/pnas.69.5.1304. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Brewer H. B., Jr, Shulman R., Herbert P., Ronan R., Wehrly K. The complete amino acid sequence of alanine apolipoprotein (apoC-3), and apolipoprotein from human plasma very low density lipoproteins. J Biol Chem. 1974 Aug 10;249(15):4975–4984. [PubMed] [Google Scholar]
  8. Brown W. V., Levy R. I., Fredrickson D. S. Further characterization of apolipoproteins from the human plasma very low density lipoproteins. J Biol Chem. 1970 Dec 25;245(24):6588–6594. [PubMed] [Google Scholar]
  9. Brown W. V., Levy R. I., Fredrickson D. S. Further separation of the apoproteins of the human plasma very low density lipoproteins. Biochim Biophys Acta. 1970 Mar 31;200(3):573–575. doi: 10.1016/0005-2795(70)90115-7. [DOI] [PubMed] [Google Scholar]
  10. Brown W. V., Levy R. I., Fredrickson D. S. Studies of the proteins in human plasma very low density lipoproteins. J Biol Chem. 1969 Oct 25;244(20):5687–5694. [PubMed] [Google Scholar]
  11. Delahunty T., Baker H. N., Gotto A. M., Jr, Jackson R. L. The primary structure of human plasma high density apolipoprotein glutamine I (ApoA-I). I. The amino acid sequence of cyanogen bromide fragment II. J Biol Chem. 1975 Apr 10;250(7):2718–2724. [PubMed] [Google Scholar]
  12. Eisenberg S., Levy R. I. Lipoprotein metabolism. Adv Lipid Res. 1975;13:1–89. [PubMed] [Google Scholar]
  13. Gotto A. M., Brown W. V., Levy R. I., Birnbaumer M. E., Fredrickson D. S. Evidence for the identity of the major apoprotein in low density and very low density lipoproteins in normal subjects and patients with familial hyperlipoproteinemia. J Clin Invest. 1972 Jun;51(6):1486–1494. doi: 10.1172/JCI106945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Havel R. J., Fielding C. J., Olivecrona T., Shore V. G., Fielding P. E., Egelrud T. Cofactor activity of protein components of human very low density lipoproteins in the hydrolysis of triglycerides by lipoproteins lipase from different sources. Biochemistry. 1973 Apr 24;12(9):1828–1833. doi: 10.1021/bi00733a026. [DOI] [PubMed] [Google Scholar]
  15. Herbert P., Levy R. I., Fredrickson D. S. Correction of COOH-terminal amino acids of human plasma very low density apolipoproteins. J Biol Chem. 1971 Nov 25;246(22):7068–7069. [PubMed] [Google Scholar]
  16. Hermodson M. A., Ericsson L. H., Titani K., Neurath H., Walsh K. A. Application of sequenator analyses to the study of proteins. Biochemistry. 1972 Nov 21;11(24):4493–4502. doi: 10.1021/bi00774a011. [DOI] [PubMed] [Google Scholar]
  17. Inagami T., Murakami K. Identification of phenylthiohydantoins of amino acids by thin-layer chromatography on a plastic-backed silica-gel plate. Anal Biochem. 1972 Jun;47(2):501–504. doi: 10.1016/0003-2697(72)90144-3. [DOI] [PubMed] [Google Scholar]
  18. Jackson R. L., Lin H. Y., Chan L., Means A. R. Amino acid sequence of a major apoprotein from hen plasma very low density lipoproteins. J Biol Chem. 1977 Jan 10;252(1):250–253. [PubMed] [Google Scholar]
  19. Jackson R. L., Morrisett J. D., Gotto A. M., Jr Lipoprotein structure and metabolism. Physiol Rev. 1976 Apr;56(2):259–316. doi: 10.1152/physrev.1976.56.2.259. [DOI] [PubMed] [Google Scholar]
  20. Jackson R. L., Morrisett J. D., Gotto A. M., Jr, Segrest J. P. The mechanism of lipid-binding by plasma lipoproteins. Mol Cell Biochem. 1975 Jan 31;6(1):43–50. doi: 10.1007/BF01731865. [DOI] [PubMed] [Google Scholar]
  21. Jackson R. L., Sparrow J. T., Baker H. N., Morrisett J. D., Taunton O. D., Gotto A. M., Jr The primary structure of apolopoprotein-serine. J Biol Chem. 1974 Aug 25;249(16):5308–5313. [PubMed] [Google Scholar]
  22. LaRosa J. C., Levy R. I., Herbert P., Lux S. E., Fredrickson D. S. A specific apoprotein activator for lipoprotein lipase. Biochem Biophys Res Commun. 1970 Oct 9;41(1):57–62. doi: 10.1016/0006-291x(70)90468-7. [DOI] [PubMed] [Google Scholar]
  23. Morrisett J. D., David J. S., Pownall H. J., Gotto A. M., Jr Interaction of an apolipoprotein (apoLP-alanine) with phosphatidylcholine. Biochemistry. 1973 Mar 27;12(7):1290–1299. doi: 10.1021/bi00731a008. [DOI] [PubMed] [Google Scholar]
  24. Morrisett J. D., Jackson R. L., Gotto A. M., Jr Lipoproteins: structure and function. Annu Rev Biochem. 1975;44:183–207. doi: 10.1146/annurev.bi.44.070175.001151. [DOI] [PubMed] [Google Scholar]
  25. Pisano J. J., Bronzert T. J., Brewer H. B., Jr Advances in the gas chromatographic analysis of amino acid phenyl- and methylthiohydantoins. Anal Biochem. 1972 Jan;45(1):43–59. doi: 10.1016/0003-2697(72)90006-1. [DOI] [PubMed] [Google Scholar]
  26. Segrest J. P., Jackson R. L., Morrisett J. D., Gotto A. M., Jr A molecular theory of lipid-protein interactions in the plasma lipoproteins. FEBS Lett. 1974 Jan 15;38(3):247–258. doi: 10.1016/0014-5793(74)80064-5. [DOI] [PubMed] [Google Scholar]
  27. Shore B., Shore V. Isolation and characterization of polypeptides of human serum lipoproteins. Biochemistry. 1969 Nov;8(11):4510–4516. doi: 10.1021/bi00839a043. [DOI] [PubMed] [Google Scholar]
  28. Shore V. G., Shore B. Heterogeneity of human plasma very low density lipoproteins. Separation of species differing in protein components. Biochemistry. 1973 Jan 30;12(3):502–507. doi: 10.1021/bi00727a022. [DOI] [PubMed] [Google Scholar]
  29. Shulman R. S., Herbert P. N., Fredrickson D. S., Wehrly K., Brewer H. B., Jr Isolation and alignment of the tryptic peptides of alanine apolipoprotein, an apolipoprotein from human plasma very low density lipoproteins. J Biol Chem. 1974 Aug 10;249(15):4969–4974. [PubMed] [Google Scholar]
  30. Shulman R. S., Herbert P. N., Wehrly K., Fredrickson D. S. Thf complete amino acid sequence of C-I (apoLp-Ser), an apolipoprotein from human very low density lipoproteins. J Biol Chem. 1975 Jan 10;250(1):182–190. [PubMed] [Google Scholar]

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