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. 1976 Oct;73(10):3544–3548. doi: 10.1073/pnas.73.10.3544

On the specificity of synapse formation.

D G Puro, M Nirenberg
PMCID: PMC431153  PMID: 185619

Abstract

Clonal neuroblastome X glioma hybrid cells form synapses with striated muscle cells from different muscles and from different organisms, such as chick, mouse, and rat. Under appropriate conditions 65-80% of the muscle cells exhibited synaptic responses. The results indicate that most striated muscle cells have the same specificity for synapse formation and suggest that synaptogenesis is not dependent on interactions between complementary molecules on neuron and muscle cells that code for different synaptic connections.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amano T., Hamprecht B., Kemper W. High activity of choline acetyltransferase induced in neuroblastoma x glia hybrid cells. Exp Cell Res. 1974 Apr;85(2):399–408. doi: 10.1016/0014-4827(74)90142-6. [DOI] [PubMed] [Google Scholar]
  2. Bennett M. R., Pettigrew A. G. The formation of synapses in striated muscle during development. J Physiol. 1974 Sep;241(2):515–545. doi: 10.1113/jphysiol.1974.sp010670. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Crain S. M., Alfei L., Peterson E. R. Neuromuscular transmission in cultures of adult human and rodent skeletal muscle after innervation in vitro by fetal rodent spinal cord. J Neurobiol. 1970;1(4):471–489. doi: 10.1002/neu.480010409. [DOI] [PubMed] [Google Scholar]
  4. DIAMOND J., MILEDI R. A study of foetal and new-born rat muscle fibres. J Physiol. 1962 Aug;162:393–408. doi: 10.1113/jphysiol.1962.sp006941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Drachman D. B., Witzke F. Trophic regulation of acetylcholine sensitivity of muscle: effect of electrical stimulation. Science. 1972 May 5;176(4034):514–516. doi: 10.1126/science.176.4034.514. [DOI] [PubMed] [Google Scholar]
  6. Frank E., Jansen J. K., Lomo T., Westgaard R. H. The interaction between foreign and original motor nerves innervating the soleus muscle of rats. J Physiol. 1975 Jun;247(3):725–743. doi: 10.1113/jphysiol.1975.sp010954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fukuda J. Chloride spike: a third type of action potential in tissue-cultured skeletal muscle cells from the chick. Science. 1974 Jul 5;185(4145):76–78. doi: 10.1126/science.185.4145.76. [DOI] [PubMed] [Google Scholar]
  8. HUBEL D. H., WIESEL T. N. RECEPTIVE FIELDS OF CELLS IN STRIATE CORTEX OF VERY YOUNG, VISUALLY INEXPERIENCED KITTENS. J Neurophysiol. 1963 Nov;26:994–1002. doi: 10.1152/jn.1963.26.6.994. [DOI] [PubMed] [Google Scholar]
  9. Kidokoro Y., Heinemann S., Schubert D., Brandt B. L., Klier F. G. Synapse formation and neurotrophic effects on muscle cell lines. Cold Spring Harb Symp Quant Biol. 1976;40:373–388. doi: 10.1101/sqb.1976.040.01.036. [DOI] [PubMed] [Google Scholar]
  10. Kidokoro Y., Heinemann S. Synapse formation between clonal muscle cells and rat spinal cord explants. Nature. 1974 Dec 13;252(5484):593–594. doi: 10.1038/252593a0. [DOI] [PubMed] [Google Scholar]
  11. Landmesser L. Contractile and electrical responses of vagus-innervated frog sartorius muscles. J Physiol. 1971 Mar;213(3):707–725. doi: 10.1113/jphysiol.1971.sp009410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lomo T., Rosenthal J. Control of ACh sensitivity by muscle activity in the rat. J Physiol. 1972 Mar;221(2):493–513. doi: 10.1113/jphysiol.1972.sp009764. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Minna J., Glazer D., Nirenberg M. Genetic dissection of neural properties using somatic cell hybrids. Nat New Biol. 1972 Feb 23;235(60):225–231. doi: 10.1038/newbio235225a0. [DOI] [PubMed] [Google Scholar]
  14. Minna J., Nelson P., Peacock J., Glazer D., Nirenberg M. Genes for neuronal properties expressed in neuroblastoma x L cell hybrids. Proc Natl Acad Sci U S A. 1971 Jan;68(1):234–239. doi: 10.1073/pnas.68.1.234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nelson P. G., Peacock J. H., Amano T., Minna J. Electrogenesis in mouse neuroblastoma cells in vitro. J Cell Physiol. 1971 Jun;77(3):337–352. doi: 10.1002/jcp.1040770308. [DOI] [PubMed] [Google Scholar]
  16. Nelson P., Christian C., Nirenberg M. Synapse formation between clonal neuroblastoma X glioma hybrid cells and striated muscle cells. Proc Natl Acad Sci U S A. 1976 Jan;73(1):123–127. doi: 10.1073/pnas.73.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nurse C. A., O'Lague P. H. Formation of cholinergic synapses between dissociated sympathetic neurons and skeletal myotubes of the rat in cell culture. Proc Natl Acad Sci U S A. 1975 May;72(5):1955–1959. doi: 10.1073/pnas.72.5.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Peterson E. R., Crain S. M. Innervation in cultures of fetal rodent skeletal muscle by organotypic explants of spinal cord from different animals. Z Zellforsch Mikrosk Anat. 1970;106(1):1–21. doi: 10.1007/BF01027714. [DOI] [PubMed] [Google Scholar]
  19. Redfern P. A. Neuromuscular transmission in new-born rats. J Physiol. 1970 Aug;209(3):701–709. doi: 10.1113/jphysiol.1970.sp009187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. SPERRY R. W. CHEMOAFFINITY IN THE ORDERLY GROWTH OF NERVE FIBER PATTERNS AND CONNECTIONS. Proc Natl Acad Sci U S A. 1963 Oct;50:703–710. doi: 10.1073/pnas.50.4.703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Stent G. S. A physiological mechanism for Hebb's postulate of learning. Proc Natl Acad Sci U S A. 1973 Apr;70(4):997–1001. doi: 10.1073/pnas.70.4.997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Vogel Z., Sytkowski A. J., Nirenberg M. W. Acetylcholine receptors of muscle grown in vitro. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3180–3184. doi: 10.1073/pnas.69.11.3180. [DOI] [PMC free article] [PubMed] [Google Scholar]

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