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. 1977 Aug;74(8):3184–3188. doi: 10.1073/pnas.74.8.3184

Presence of a putative 15S precursor to β-globin mRNA but not to α-globin mRNA in Friend cells

Peter J Curtis 1, Ned Mantei 1, Johan Van Den Berg 1, Charles Weissmann 1
PMCID: PMC431488  PMID: 410017

Abstract

Dimethyl sulfoxide-induced Friend cells were labeled for periods of 5-60 min. The denatured RNA was fractionated by sucrose gradient centrifugation and the distribution of α- and β-globin-specific [3H]RNA was determined by hybridization to hybrid plasmids containing mouse α- and β-globin DNA, respectively. After 5 min of labeling, a 15S peak of β-globin-specific (but not α-globin-specific) [3H]RNA was detected, next to an equal amount of 10S β-globin [3H]RNA. With increasing periods of labeling, the amount of 15S β-globin [3H]RNA remained constant but the amount 10S β-globin [3H]RNA increased steadily. α-Globin-specific [3H]RNA sedimented at 11 S after 5 min of labeling and at 9.5 S after longer labeling periods. Analysis of 15S globin-specific [3H]RNA purified by the poly(dC)-cDNA method [Curtis, P. J. & Weissmann, C. (1976) J. Mol. Biol. 106, 1061-1075] showed oligonucleotides characteristic of β-globin mRNA but not of α-globin mRNA, as well as about 20 new oligonucleotides. Our results suggest that 10S β-globin mRNA arises via a 15S precursor that has a half-life of 5 min or less; 9.5S α-globin mRNA may be derived from an 11S precursor.

Keywords: globin mRNA precursor, mouse globin cDNA plasmid

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Selected References

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