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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1977 Aug;74(8):3429–3432. doi: 10.1073/pnas.74.8.3429

Fibroblast heterogeneity and prostaglandin regulation of subpopulations

S David Ko 1,2,3, Roy C Page 1,2,3, A S Narayanan 1,2,3
PMCID: PMC431592  PMID: 269402

Abstract

The effects of prostaglandin E2 (PGE2) upon the synthesis of protein and DNA, and membrane transport of proline and thymidine, by human diploid fibroblasts were studied. At a concentration range of 1-10 μM, PGE2 inhibited protein synthesis and membrane transport by 45-50%. Serum-activated DNA synthesis and thymidine transport were also inhibited by approximately 50% in cells made quiescent and synchronous by serum deprivation. To determine whether prostaglandin inhibits some of the cells completely or all of the cells partially, radioautographic and cell-counting experiments were done. In cultures pulse-labeled with [3H]thymidine 12-33 hr after serum activation, prostaglandin exposure reduced the number of labeled nuclei by 42%. Sixty-five hours after serum activation, the total cell numbers present in the PGE2-exposed cultures were reduced by 25%. Furthermore, in the fibroblast cultures derived from cells previously maintained in 10 μM PGE2 for 14 days, PGE2 had no effect on DNA synthesis, indicating that the PGE2-sensitive cells had disappeared from the cultures. Thus, PGE2 appears to inhibit growth and synthesis of a subpopulation of cells while not affecting the remaining insensitive cells. Prostaglandins may play an important role in connective-tissue differentiation and in some pathologic alterations by regulating fibroblast subpopulations.

Keywords: DNA synthesis, mitosis, connective tissue, collagen, gingiva

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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