Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1977 Sep;74(9):4055–4058. doi: 10.1073/pnas.74.9.4055

Alteration of cellular adhesion by nerve growth factor.

D Schubert, C Whitlock
PMCID: PMC431843  PMID: 198815

Abstract

Nerve growth factor increases the initial rates of cell-substratum and cell-cell adhesion of sympathetic nerve cell clone PC12. It also stimulates adenosine 3':5'-cyclic monophosphate accumulation in the cells, and exogenous adenosine 3':5'-cyclic monophosphate increases cell-substratum adhesion. NGH-stimulated adhesion is energy dependent and cold-sensitive and does not require de novo protein synthesis. It is probably mediated by an effect of adenosine 3':5'-cyclic monophosphate on the plasma membrane. Stimulation of cellular adhesion may be the first step in NGF-initiated neurite outgrowth by sympathetic nerve cells.

Full text

PDF
4058

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauduin H., Stock C., Vincent D., Grenier J. F. Microfilamentous system and secretion of enzyme in the exocrine pancreas. Effect of cytochalasin B. J Cell Biol. 1975 Jul;66(1):165–181. doi: 10.1083/jcb.66.1.165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boyd L. F., Bradshaw R. A., Frazier W. A., Hogue-Angeletti R. A., Jeng I. M., Pulliam M. W., Szutowicz A. Nerve growth factor. Life Sci. 1974 Oct 15;15(8):1381–1391. doi: 10.1016/0024-3205(74)90113-1. [DOI] [PubMed] [Google Scholar]
  3. Eagle H. Buffer combinations for mammalian cell culture. Science. 1971 Oct 29;174(4008):500–503. doi: 10.1126/science.174.4008.500. [DOI] [PubMed] [Google Scholar]
  4. Furmanski P., Silverman D. J., Lubin M. Expression of differentiated functions in mouse neuroblastoma mediated by dibutyryl-cyclic adenosine monophosphate. Nature. 1971 Oct 8;233(5319):413–415. doi: 10.1038/233413a0. [DOI] [PubMed] [Google Scholar]
  5. Greene L. A., Tischler A. S. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2424–2428. doi: 10.1073/pnas.73.7.2424. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Grinnell F. Studies on the mechanism of cell attachment to a substratum with serum in the medium: further evidence supporting a requirement for two biochemically distinct processes. Arch Biochem Biophys. 1974 Dec;165(2):524–530. doi: 10.1016/0003-9861(74)90278-1. [DOI] [PubMed] [Google Scholar]
  7. Grinnell F. Studies on the mechanism of cell attachment to a substratum: evidence for three biochemically distinct processes. Arch Biochem Biophys. 1974 Jan;160(1):304–310. doi: 10.1016/s0003-9861(74)80038-x. [DOI] [PubMed] [Google Scholar]
  8. HEYTLER P. G. uncoupling of oxidative phosphorylation by carbonyl cyanide phenylhydrazones. I. Some characteristics of m-Cl-CCP action on mitochondria and chloroplasts. Biochemistry. 1963 Mar-Apr;2:357–361. doi: 10.1021/bi00902a031. [DOI] [PubMed] [Google Scholar]
  9. Hill C. E., Hendry I. A. Differences in sensitivity to nerve growth factor of axon formation and tyrosine hydroxylase induction in cultured sympathetic neurons. Neuroscience. 1976 Dec;1(6):489–496. doi: 10.1016/0306-4522(76)90101-9. [DOI] [PubMed] [Google Scholar]
  10. Kidokoro Y., Heinemann S., Schubert D., Brandt B. L., Klier F. G. Synapse formation and neurotrophic effects on muscle cell lines. Cold Spring Harb Symp Quant Biol. 1976;40:373–388. doi: 10.1101/sqb.1976.040.01.036. [DOI] [PubMed] [Google Scholar]
  11. Kolodny G. M. Effect of various inhibitors of readhesion of trypsinized cells in culture. Exp Cell Res. 1972 Jan;70(1):196–202. doi: 10.1016/0014-4827(72)90197-8. [DOI] [PubMed] [Google Scholar]
  12. Lyman G. H., Preisler H. D., Papahadjopoulos D. Membrane action of DMSO and other chemical inducers of Friend leukaemic cell differentiation. Nature. 1976 Jul 29;262(5567):361–363. doi: 10.1038/262360a0. [DOI] [PubMed] [Google Scholar]
  13. Merrell R., Pulliam M. W., Randono L., Boyd L. F., Bradshaw R. A., Glaser L. Temporal changes in tectal cell surface specificity induced by nerve growth factor. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4270–4274. doi: 10.1073/pnas.72.11.4270. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nelson P., Christian C., Nirenberg M. Synapse formation between clonal neuroblastoma X glioma hybrid cells and striated muscle cells. Proc Natl Acad Sci U S A. 1976 Jan;73(1):123–127. doi: 10.1073/pnas.73.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Obrink B., Kuhlenschmidt M. S., Roseman S. Adhesive specificity of juvenile rat and chicken liver cells and membranes. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1077–1081. doi: 10.1073/pnas.74.3.1077. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schubert D., Heinemann S., Carlisle W., Tarikas H., Kimes B., Patrick J., Steinbach J. H., Culp W., Brandt B. L. Clonal cell lines from the rat central nervous system. Nature. 1974 May 17;249(454):224–227. doi: 10.1038/249224a0. [DOI] [PubMed] [Google Scholar]
  17. Schubert D., Heinemann S., Kidokoro Y. Cholinergic metabolism and synapse formation by a rat nerve cell line. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2579–2583. doi: 10.1073/pnas.74.6.2579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schubert D., Humphreys S., Jacob F., de Vitry F. Induced differentiation of a neuroblastoma. Dev Biol. 1971 Aug;25(4):514–546. doi: 10.1016/0012-1606(71)90004-2. [DOI] [PubMed] [Google Scholar]
  19. Stallcup W. B. Specificity of adhesion between cloned neural cell lines. Brain Res. 1977 May 13;126(3):475–486. doi: 10.1016/0006-8993(77)90598-4. [DOI] [PubMed] [Google Scholar]
  20. Tarikas H., Schubert D. Regulation of adenylate kinase and creatine kinase activities in myogenic cells. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2377–2381. doi: 10.1073/pnas.71.6.2377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Watson J. The influence of intracellular levels of cyclic nucleotides on cell proliferation and the induction of antibody synthesis. J Exp Med. 1975 Jan 1;141(1):97–111. doi: 10.1084/jem.141.1.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Yaffe D. Retention of differentiation potentialities during prolonged cultivation of myogenic cells. Proc Natl Acad Sci U S A. 1968 Oct;61(2):477–483. doi: 10.1073/pnas.61.2.477. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES