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. 1977 Oct;74(10):4332–4336. doi: 10.1073/pnas.74.10.4332

DNA sequence organization in the water mold Achlya

M E S Hudspeth *,†, William E Timberlake *,†, Robert B Goldberg *,†,‡
PMCID: PMC431935  PMID: 16592446

Abstract

Experiments are described that characterize the organization of DNA sequences in the water mold Achlya bisexualis. These experiments demonstrate that repetitive and single copy sequences in the Achlya genome are arranged in a long-period interspersion pattern. Estimates of the spacing intervals between repetitive and single copy DNA indicate, however, that the interspersion pattern in Achlya is longer than has been previously reported in other eukaryotes. These data and measurements of structural gene expression in Achlya [Timberlake, W.E., Shumard, D. S. & Goldberg, R. B. (1977) Cell 10, 623-632] make it difficult to propose a regulatory function for repeated DNA in this eukaryote.

Keywords: reassociation kinetics, interspersion, fungi

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Britten R. J., Graham D. E., Eden F. C., Painchaud D. M., Davidson E. H. Evolutionary divergence and length of repetitive sequences in sea urchin DNA. J Mol Evol. 1976 Dec 31;9(1):1–23. doi: 10.1007/BF01796119. [DOI] [PubMed] [Google Scholar]
  2. Cockburn A. F., Newkirk M. J., Firtel R. A. Organization of the ribosomal RNA genes of Dictyostelium discoideum: mapping of the nontranscribed spacer regions. Cell. 1976 Dec;9(4 Pt 1):605–613. doi: 10.1016/0092-8674(76)90043-x. [DOI] [PubMed] [Google Scholar]
  3. Crain W. R., Davidson E. H., Britten R. J. Contrasting patterns of DNA sequence arrangement in Apis mellifera (honeybee) and Musca domestica (housefly). Chromosoma. 1976 Dec 6;59(1):1–12. doi: 10.1007/BF00327705. [DOI] [PubMed] [Google Scholar]
  4. Crain W. R., Eden F. C., Pearson W. R., Davidson E. H., Britten R. J. Absence of short period interspersion of repetitive and non-repetitive sequences in the DNA of Drosophila melanogaster. Chromosoma. 1976 Jul 30;56(4):309–326. doi: 10.1007/BF00292953. [DOI] [PubMed] [Google Scholar]
  5. Davidson E. H., Galau G. A., Angerer R. C., Britten R. J. Comparative aspects of DNA organization in Metazoa. Chromosoma. 1975 Jul 21;51(3):253–259. doi: 10.1007/BF00284818. [DOI] [PubMed] [Google Scholar]
  6. Davidson E. H., Hough B. R., Amenson C. S., Britten R. J. General interspersion of repetitive with non-repetitive sequence elements in the DNA of Xenopus. J Mol Biol. 1973 Jun 15;77(1):1–23. doi: 10.1016/0022-2836(73)90359-8. [DOI] [PubMed] [Google Scholar]
  7. Davidson E. H., Klein W. H., Britten R. J. Sequence organization in animal DNA and a speculation on hnRNA as a coordinate regulatory transcript. Dev Biol. 1977 Jan;55(1):69–84. doi: 10.1016/0012-1606(77)90320-7. [DOI] [PubMed] [Google Scholar]
  8. Dusenbery R. L. Characterization of the genome of Phycomyces blakesleeanus. Biochim Biophys Acta. 1975 Feb 10;378(3):363–377. doi: 10.1016/0005-2787(75)90181-1. [DOI] [PubMed] [Google Scholar]
  9. Firtel R. A., Kindle K. Structural organization of the genome of the cellular slime mold Dictyostelium discoideum: interspersion of repetitive and single-copy DNA sequences. Cell. 1975 Aug;5(4):401–411. doi: 10.1016/0092-8674(75)90059-8. [DOI] [PubMed] [Google Scholar]
  10. Goldberg R. B., Crain W. R., Ruderman J. V., Moore G. P., Barnett T. R., Higgins R. C., Gelfand R. A., Galau G. A., Britten R. J., Davidson E. H. DNA sequence organization in the genomes of five marine invertebrates. Chromosoma. 1975 Jul 21;51(3):225–251. doi: 10.1007/BF00284817. [DOI] [PubMed] [Google Scholar]
  11. Jaworski A. J., Horgen P. A. The ribosomal cistrons of the water mold Achlya bisexualis. Arch Biochem Biophys. 1973 Jul;157(1):260–267. doi: 10.1016/0003-9861(73)90412-8. [DOI] [PubMed] [Google Scholar]
  12. Maizels N. Dictyostelium 17S, 25S, and 5S rDNAs lie within a 38,000 base pair repeated unit. Cell. 1976 Nov;9(3):431–438. doi: 10.1016/0092-8674(76)90088-x. [DOI] [PubMed] [Google Scholar]
  13. Manning J. E., Schmid C. W., Davidson N. Interspersion of repetitive and nonrepetitive DNA sequences in the Drosophila melanogaster genome. Cell. 1975 Feb;4(2):141–155. doi: 10.1016/0092-8674(75)90121-x. [DOI] [PubMed] [Google Scholar]
  14. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  15. Smith M. J., Britten R. J., Davidson E. H. Studies on nucleic acid reassociation kinetics: reactivity of single-stranded tails in DNA-DNA renaturation. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4805–4809. doi: 10.1073/pnas.72.12.4805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Timberlake W. E. Alterations in RNA and protein synthesis associated with steroid hormone-induced sexual morphogenesis in the water mold Achlya. Dev Biol. 1976 Jul 15;51(2):202–214. doi: 10.1016/0012-1606(76)90138-x. [DOI] [PubMed] [Google Scholar]
  17. Timberlake W. E., Shumard D. S., Goldberg R. B. Relationship between nuclear and polysomal RNA populations of Achlya: a simple eucaryotic system. Cell. 1977 Apr;10(4):623–632. doi: 10.1016/0092-8674(77)90095-2. [DOI] [PubMed] [Google Scholar]
  18. Wells R., Royer H. D., Hollenberg C. P. Non Xenopus-like DNA sequence organization in the Chironomus tentans genome. Mol Gen Genet. 1976 Aug 10;147(1):45–51. doi: 10.1007/BF00337934. [DOI] [PubMed] [Google Scholar]
  19. Wetmur J. G., Davidson N. Kinetics of renaturation of DNA. J Mol Biol. 1968 Feb 14;31(3):349–370. doi: 10.1016/0022-2836(68)90414-2. [DOI] [PubMed] [Google Scholar]

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