Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1977 Nov;74(11):5060–5064. doi: 10.1073/pnas.74.11.5060

Hexamethylene bisacetamide induces morphologic changes and increased synthesis of procollagen in cell line from glioblastoma multiforme.

A S Rabson, R Stern, T S Tralka, J Costa, J Wilczek
PMCID: PMC432098  PMID: 200944

Abstract

Addition to hexamethylene bisacetamide (diacetyldiaminohexane) to cultures of a malignant mesenchymal cell line derived from a human glioblastoma multiforme induces morphological changes and stimulates the synthesis of procollagen. The morphological changes include cell elongation, an increase of extracellular material with staining properties of collagen by light microscopy, and an increase in extracellular 220-A fibrils by electron microscopy. The rate of procollagen synthesis increased as much as 20-fold, and the ratio of type I:type III procollagen changed, with type I becoming the predominant form. The change in type I:type III ratio is similar to that seen in the maturation of normal fetal to adult connective tissue.

Full text

PDF
5061

Images in this article

5061Image
on p.5061
5061Image
on p.5061
5062Image
on p.5062

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cutroneo K. R., Guzman N. A., Liebelt A. G. Elevation of peptidylproline hydroxylase activity and collagen synthesis in spontaneous primary mammary cancers of inbred mice. Cancer Res. 1972 Dec;32(12):2828–2833. [PubMed] [Google Scholar]
  2. Epstein E. H., Jr (Alpha1(3))3 human skin collagen. Release by pepsin digestion and preponderance in fetal life. J Biol Chem. 1974 May 25;249(10):3225–3231. [PubMed] [Google Scholar]
  3. Evans C. A., Peterkofsky B. Ascorbate-independent proline hydroxylation resulting from viral transformation of Balb 3T3 cells and unaffected by dibutyryl cAMP treatment. J Cell Physiol. 1976 Nov;89(3):355–367. doi: 10.1002/jcp.1040890302. [DOI] [PubMed] [Google Scholar]
  4. GREEN H., GOLDBERG B. COLLAGEN SYNTHESIS BY HUMAN FIBROBLAST STRAINS. Proc Soc Exp Biol Med. 1964 Oct;117:258–261. doi: 10.3181/00379727-117-29551. [DOI] [PubMed] [Google Scholar]
  5. Gay S., Martin G. R., Muller P. K., Timpl R., Kuhn K. Simultaneous synthesis of types I and III collagen by fibroblasts in culture. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4037–4040. doi: 10.1073/pnas.73.11.4037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Johnson G. S., Friedman R. M., Pastan I. Restoration of several morphological characteristics of normal fibroblasts in sarcoma cells treated with adenosine-3':5'-cyclic monphosphate and its derivatives. Proc Natl Acad Sci U S A. 1971 Feb;68(2):425–429. doi: 10.1073/pnas.68.2.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LeRoy E. C. Increased collagen synthesis by scleroderma skin fibroblasts in vitro: a possible defect in the regulation or activation of the scleroderma fibroblast. J Clin Invest. 1974 Oct;54(4):880–889. doi: 10.1172/JCI107827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Miller E. J., Matukas V. J. Biosynthesis of collagen. The biochemist's view. Fed Proc. 1974 May;33(5):1197–1204. [PubMed] [Google Scholar]
  9. Nudel U., Salmon J. E., Terada M., Bank A., Rifkind R. A., Marks P. A. Differential effects of chemical inducers on expression of beta globin genes in murine erythroleukemia cells. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1100–1104. doi: 10.1073/pnas.74.3.1100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rabson A. S., O'Conor G. T., Lorenz D. E., Kirschstein R. L., Legallais F. Y., Tralka T. S. Lymphoid cell-culture line derived from lymph node of marmoset infected wtih Herpesvirus saimiri--preliminary report. J Natl Cancer Inst. 1971 May;46(5):1099–1109. [PubMed] [Google Scholar]
  11. Reuben R. C., Wife R. L., Breslow R., Rifkind R. A., Marks P. A. A new group of potent inducers of differentiation in murine erythroleukemia cells. Proc Natl Acad Sci U S A. 1976 Mar;73(3):862–866. doi: 10.1073/pnas.73.3.862. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Smith B. D., Byers P. H., Martin G. R. Production of procollagen by human fibroblasts in culture. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3260–3262. doi: 10.1073/pnas.69.11.3260. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sykes B., Francis M. J., Smith R. Altered relation of two collagen types in osteogenesis imperfecta. N Engl J Med. 1977 May 26;296(21):1200–1203. doi: 10.1056/NEJM197705262962104. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES