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. 1977 Jun;74(6):2259–2263. doi: 10.1073/pnas.74.6.2259

Synthesis of opiate peptides by a clonal pituitary tumor cell line.

G Giagnoni, S L Sabol, M Nirenberg
PMCID: PMC432149  PMID: 267923

Abstract

Clonal mouse pituitary tumor cells, AtT-20, synthesize at least four species of peptides with opiate activity. The endorphin concentration of AtT-20 cells was estimated to be 300-600 pmol/mg of protein. The two most abundant endorphins with apparent molecular weights of 1800 and 2400 were purified 300- and 24-fold, respectively; additional minor components were found with apparent molecular weights of greater than 3000 and less than 750.

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Selected References

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