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. 1975 Jan;72(1):235–239. doi: 10.1073/pnas.72.1.235

Recombinant DNA molecules of bacteriophage phi chi174.

R M Benbow, A J Zuccarelli, R L Sinsheimer
PMCID: PMC432278  PMID: 1054500

Abstract

Phi chi174 DNA structures containing two different parental genomes were detected genetically and examined by electron microscopy. These structures consisted of two monomeric double-stranded DNA molecules linked in a figure 8 configuration. Such DNA structures were observed to be formed preferentially in host recA+ cells or recA+ cell-free systems. Since the host recA+ allele is required for most phi chi174 recombinant formation, we conclude that the observed figure 8 molecules are intermediates in, or end products of, 1 phi chi174 recombination event. We propose that recombinant figure 8 DNA molecules arise as a result of "single-strand aggression," are stabilized by double-strand "branch migration," and represent a specific example of a common intermediate in genetic recombination.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baas P. D., Jansz H. S. Asymmetric information transfer during phi X174 DNA replication. J Mol Biol. 1972 Feb 14;63(3):557–568. doi: 10.1016/0022-2836(72)90447-0. [DOI] [PubMed] [Google Scholar]
  2. Baas P. D., Jansz H. S. PhiX174 replicative form DNA replication, origin and direction. J Mol Biol. 1972 Feb 14;63(3):569–576. doi: 10.1016/0022-2836(72)90448-2. [DOI] [PubMed] [Google Scholar]
  3. Baker R., Doniger J., Tessman I. Roles of parental and progeny DNA in 2 mechanisms of phage S13 recombination. Nat New Biol. 1971 Mar 3;230(1):23–25. doi: 10.1038/newbio230023a0. [DOI] [PubMed] [Google Scholar]
  4. Baker R., Tessman I. The circular genetic map of phage S13. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1438–1445. doi: 10.1073/pnas.58.4.1438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Benbow R. M., Eisenberg M., Sinsheimer R. L. Multiple length DNA molecules of bacteriophage phi-X174. Nat New Biol. 1972 May 31;237(74):141–144. doi: 10.1038/newbio237141a0. [DOI] [PubMed] [Google Scholar]
  6. Benbow R. M., Hutchison C. A., Fabricant J. D., Sinsheimer R. L. Genetic Map of Bacteriophage phiX174. J Virol. 1971 May;7(5):549–558. doi: 10.1128/jvi.7.5.549-558.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Benbow R. M., Mayol R. F., Picchi J. C., Sinsheimer R. L. Direction of Translation and Size of Bacteriophage phiX174 Cistrons. J Virol. 1972 Jul;10(1):99–114. doi: 10.1128/jvi.10.1.99-114.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Benbow R. M., Zuccarelli A. J., Davis G. C., Sinsheimer R. L. Genetic recombination in bacteriophage phi chi 174. J Virol. 1974 Apr;13(4):898–907. doi: 10.1128/jvi.13.4.898-907.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Benbow R. M., Zuccarelli A. J., Sinsheimer R. L. A role for single-strand breaks in bacteriophage phi-X174 genetic recombination. J Mol Biol. 1974 Sep 25;88(3):629–651. doi: 10.1016/0022-2836(74)90414-8. [DOI] [PubMed] [Google Scholar]
  10. Boon T., Zinder N. D. Genotypes produced by individual recombination events involving bacteriophage f1. J Mol Biol. 1971 May 28;58(1):133–151. doi: 10.1016/0022-2836(71)90237-3. [DOI] [PubMed] [Google Scholar]
  11. Broker T. R., Lehman I. R. Branched DNA molecules: intermediates in T4 recombination. J Mol Biol. 1971 Aug 28;60(1):131–149. doi: 10.1016/0022-2836(71)90453-0. [DOI] [PubMed] [Google Scholar]
  12. Clayton D. A., Davis R. W., Vinograd J. Homology and structural relationships between the dimeric and monomeric circular forms of mitochondrial DNA from human leukemic leukocytes. J Mol Biol. 1970 Jan 28;47(2):137–153. doi: 10.1016/0022-2836(70)90335-9. [DOI] [PubMed] [Google Scholar]
  13. Doniger J., Warner R. C., Tessma I. Role of circular dimer DNA in the primary recombination mechanism of bacteriophage S13. Nat New Biol. 1973 Mar 7;242(114):9–12. doi: 10.1038/newbio242009a0. [DOI] [PubMed] [Google Scholar]
  14. Gordon C. N., Rush M. G., Warner R. C. Complex replicative form molecules of bacteriophages phi X174 and S13 su105. J Mol Biol. 1970 Feb 14;47(3):495–503. doi: 10.1016/0022-2836(70)90317-7. [DOI] [PubMed] [Google Scholar]
  15. Hofs E. B., van de Pol J. H., van Arkel G. A., Jansz H. S. Dimeric circular duplex DNA of bacteriophage phiX174 and recombination. Mol Gen Genet. 1972;118(2):161–172. doi: 10.1007/BF00267085. [DOI] [PubMed] [Google Scholar]
  16. Hotchkiss R. D. Toward a general theory of genetic recombination in DNA. Adv Genet. 1971;16:325–348. [PubMed] [Google Scholar]
  17. Kim J., Sharp P. A., Davidson N. Electron microscope studies of heteroduplex DNA from a deletion mutant of bacteriophage phiX-174. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1948–1952. doi: 10.1073/pnas.69.7.1948. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lee C. S., Davis R. W., Davidson N. A physical study by electron microscopy of the terminally reptitious, circularly permuted DNA from the coliphage particles of Escherichia coli 15. J Mol Biol. 1970 Feb 28;48(1):1–22. doi: 10.1016/0022-2836(70)90215-9. [DOI] [PubMed] [Google Scholar]
  19. Mount D. W. Isolation and genetic analysis of a strain of Escherichia coli K-12 with an amber recA mutation. J Bacteriol. 1971 Jul;107(1):388–389. doi: 10.1128/jb.107.1.388-389.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. PFEIFER D. Genetic recombination in bacteriophage phi-X 174. Nature. 1961 Feb 4;189:422–423. doi: 10.1038/189422a0. [DOI] [PubMed] [Google Scholar]
  21. PFEIFER D. [Genetic studies on the bacteriophage phi-X174. I. Development of a selective system and demonstration of a genetic recombination]. Z Vererbungsl. 1961;92:317–329. [PubMed] [Google Scholar]
  22. Rush M. G., Warner R. C. Molecular recombination in a circular genome-phi X174 and S13. Cold Spring Harb Symp Quant Biol. 1968;33:459–466. doi: 10.1101/sqb.1968.033.01.053. [DOI] [PubMed] [Google Scholar]
  23. Rush M. G., Warner R. C. Multiple length rings of phi-X174 and S13 replicative forms. 3. A possible intermediate in recombination. J Biol Chem. 1968 Sep 25;243(18):4821–4826. [PubMed] [Google Scholar]
  24. SINSHEIMER R. L., STARMAN B., NAGLER C., GUTHRIE S. The process of infection with bacteriophage phi-XI74. I. Evidence for a "replicative form". J Mol Biol. 1962 Mar;4:142–160. doi: 10.1016/s0022-2836(62)80047-3. [DOI] [PubMed] [Google Scholar]
  25. Sigal N., Alberts B. Genetic recombination: the nature of a crossed strand-exchange between two homologous DNA molecules. J Mol Biol. 1972 Nov 28;71(3):789–793. doi: 10.1016/s0022-2836(72)80039-1. [DOI] [PubMed] [Google Scholar]
  26. Spatz H. C., Trautner T. A. One way to do experiments on gene conversion? Transfection with heteroduplex SPP1 DNA. Mol Gen Genet. 1970;109(1):84–106. doi: 10.1007/BF00334048. [DOI] [PubMed] [Google Scholar]
  27. TESSMAN E. S., TESSMAN I. Genetic recombination in phage S13. Virology. 1959 Apr;7(4):465–467. doi: 10.1016/0042-6822(59)90075-3. [DOI] [PubMed] [Google Scholar]
  28. Tessman I. Genetic recombination of phage S13 in a recombination-deficient mutant of Escherichia coli K12. Biochem Biophys Res Commun. 1966 Jan 24;22(2):169–174. doi: 10.1016/0006-291x(66)90427-x. [DOI] [PubMed] [Google Scholar]
  29. Tessman I. Selective stimulation of one of the mechanisms for genetic recombination of bacteriophage S13. Science. 1968 Aug 2;161(3840):481–482. doi: 10.1126/science.161.3840.481. [DOI] [PubMed] [Google Scholar]
  30. Weisbeek P. J., van de Pol J. H. Bological activity of phi X174 replicative form DNA fragments. Biochim Biophys Acta. 1970 Dec 14;224(2):328–338. [PubMed] [Google Scholar]
  31. Yarus M. J., Sinsheimer R. L. The process of infection with bacteriophage phiX174. 8. Evidence for an essential bacterial "site". J Virol. 1967 Feb;1(1):135–144. doi: 10.1128/jvi.1.1.135-144.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Zuccarelli A. J., Benbow R. M., Sinsheimer R. L. Deletion mutants of bacteriophage phiX174. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1905–1910. doi: 10.1073/pnas.69.7.1905. [DOI] [PMC free article] [PubMed] [Google Scholar]

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