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. 1975 Jan;72(1):318–322. doi: 10.1073/pnas.72.1.318

Methylated nucleotides block 5'-terminus of vaccinia virus messenger RNA.

C M Wei, B Moss
PMCID: PMC432296  PMID: 164018

Abstract

Studies on the nature and location of the methylated nucleotides in mRNA synthesized in vitro by vaccinia virus particles revealed an unusual 5'-terminal structure. Evidence that the pyrophosphate group is blocked by 7-methylguanosine and that both 2'-O-methyl-adenosine and 2'-O-methylguanosine occupy penultimate positions was presented. According to this model, the 5'-termini of vaccinia virus mRNAs are: 7MeG-5'ppp-5'GMepNp and 7MeG-5'AMepNp.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Culp L. A., Brown G. M. Transfer ribonucleic acid methylases of HeLa cells. Arch Biochem Biophys. 1968 Mar 20;124(1):483–492. doi: 10.1016/0003-9861(68)90356-1. [DOI] [PubMed] [Google Scholar]
  2. Furuichi Y. "Methylation-coupled" transcription by virus-associated transcriptase of cytoplasmic polyhedrosis virus containing double-stranded RNA. Nucleic Acids Res. 1974 Jun;1(6):809–822. doi: 10.1093/nar/1.6.809. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kates J., Beeson J. Ribonucleic acid synthesis in vaccinia virus. I. The mechanism of synthesis and release of RNA in vaccinia cores. J Mol Biol. 1970 May 28;50(1):1–18. doi: 10.1016/0022-2836(70)90100-2. [DOI] [PubMed] [Google Scholar]
  4. Miura K., Watanabe K., Sugiura M. 5'-Terminal nucleotide sequences of the double-stranded RNA of silkworm cytoplasmic polyhedrosis virus. J Mol Biol. 1974 Jun 15;86(1):31–48. doi: 10.1016/s0022-2836(74)80005-7. [DOI] [PubMed] [Google Scholar]
  5. Miura K., Watanabe K., Sugiura M., Shatkin A. J. The 5'-terminal nucleotide sequences of the double-stranded RNA of human reovirus. Proc Natl Acad Sci U S A. 1974 Oct;71(10):3979–3983. doi: 10.1073/pnas.71.10.3979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Shatkin A. J. Methylated messenger RNA synthesis in vitro by purified reovirus. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3204–3207. doi: 10.1073/pnas.71.8.3204. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Wei C. M., Moss B. Methylation of newly synthesized viral messenger RNA by an enzyme in vaccinia virus. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3014–3018. doi: 10.1073/pnas.71.8.3014. [DOI] [PMC free article] [PubMed] [Google Scholar]

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