Abstract
Studies on the nature and location of the methylated nucleotides in mRNA synthesized in vitro by vaccinia virus particles revealed an unusual 5'-terminal structure. Evidence that the pyrophosphate group is blocked by 7-methylguanosine and that both 2'-O-methyl-adenosine and 2'-O-methylguanosine occupy penultimate positions was presented. According to this model, the 5'-termini of vaccinia virus mRNAs are: 7MeG-5'ppp-5'GMepNp and 7MeG-5'AMepNp.
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- Culp L. A., Brown G. M. Transfer ribonucleic acid methylases of HeLa cells. Arch Biochem Biophys. 1968 Mar 20;124(1):483–492. doi: 10.1016/0003-9861(68)90356-1. [DOI] [PubMed] [Google Scholar]
- Furuichi Y. "Methylation-coupled" transcription by virus-associated transcriptase of cytoplasmic polyhedrosis virus containing double-stranded RNA. Nucleic Acids Res. 1974 Jun;1(6):809–822. doi: 10.1093/nar/1.6.809. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kates J., Beeson J. Ribonucleic acid synthesis in vaccinia virus. I. The mechanism of synthesis and release of RNA in vaccinia cores. J Mol Biol. 1970 May 28;50(1):1–18. doi: 10.1016/0022-2836(70)90100-2. [DOI] [PubMed] [Google Scholar]
- Miura K., Watanabe K., Sugiura M. 5'-Terminal nucleotide sequences of the double-stranded RNA of silkworm cytoplasmic polyhedrosis virus. J Mol Biol. 1974 Jun 15;86(1):31–48. doi: 10.1016/s0022-2836(74)80005-7. [DOI] [PubMed] [Google Scholar]
- Miura K., Watanabe K., Sugiura M., Shatkin A. J. The 5'-terminal nucleotide sequences of the double-stranded RNA of human reovirus. Proc Natl Acad Sci U S A. 1974 Oct;71(10):3979–3983. doi: 10.1073/pnas.71.10.3979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shatkin A. J. Methylated messenger RNA synthesis in vitro by purified reovirus. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3204–3207. doi: 10.1073/pnas.71.8.3204. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wei C. M., Moss B. Methylation of newly synthesized viral messenger RNA by an enzyme in vaccinia virus. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3014–3018. doi: 10.1073/pnas.71.8.3014. [DOI] [PMC free article] [PubMed] [Google Scholar]