Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1975 Feb;72(2):581–585. doi: 10.1073/pnas.72.2.581

Isolation of the bacteriophage lambda A-gene protein.

A Becker, M Gold
PMCID: PMC432357  PMID: 1054839

Abstract

An in vitro assay for measuring the activity of the phage lambda A-gene product has been developed. The assay is based on the observation that A-donor extracts complement A minus extracts for packaging of exogenous immature lambda DNA into phage particles. A partial purification of the A-gene product activity using this assay is presented. A method is suggested by which this A protein-dependent in vitro system might be manipulated to analyze the mechanism of reforming the lambda cohesive termini during chromosome assimilation into phage precursors.

Full text

PDF
585

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Becker A. An endodeoxyribonuclease induced in E. coli by infection with lambda-pbio transducing phages. Biochem Biophys Res Commun. 1970 Oct 9;41(1):63–70. doi: 10.1016/0006-291x(70)90469-9. [DOI] [PubMed] [Google Scholar]
  2. Carter B. J., Shaw B. D., Smith M. G. Two stages in the replication of bacteriophage lambda DNA. Biochim Biophys Acta. 1969 Dec 16;195(2):494–505. doi: 10.1016/0005-2787(69)90656-x. [DOI] [PubMed] [Google Scholar]
  3. Dove W. F. Action of the lambda chromosome. I. Control of functions late in bacteriophage development. J Mol Biol. 1966 Aug;19(1):187–201. doi: 10.1016/s0022-2836(66)80060-8. [DOI] [PubMed] [Google Scholar]
  4. Emmons S. W. Bacteriophage lambda derivatives carrying two copies of the cohesive end site. J Mol Biol. 1974 Mar 15;83(4):511–525. doi: 10.1016/0022-2836(74)90511-7. [DOI] [PubMed] [Google Scholar]
  5. Enquist L. W., Skalka A. Replication of bacteriophage lambda DNA dependent on the function of host and viral genes. I. Interaction of red, gam and rec. J Mol Biol. 1973 Apr 5;75(2):185–212. doi: 10.1016/0022-2836(73)90016-8. [DOI] [PubMed] [Google Scholar]
  6. Gellert M. Formation of covalent circles of lambda DNA by E. coli extracts. Proc Natl Acad Sci U S A. 1967 Jan;57(1):148–155. doi: 10.1073/pnas.57.1.148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Georgopoulos C. P., Hendrix R. W., Casjens S. R., Kaiser A. D. Host participation in bacteriophage lambda head assembly. J Mol Biol. 1973 May 5;76(1):45–60. doi: 10.1016/0022-2836(73)90080-6. [DOI] [PubMed] [Google Scholar]
  8. HERSHEY A. D., BURGI E. COMPLEMENTARY STRUCTURE OF INTERACTING SITES AT THE ENDS OF LAMBDA DNA MOLECULES. Proc Natl Acad Sci U S A. 1965 Feb;53:325–328. doi: 10.1073/pnas.53.2.325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hohn B., Hohn T. Activity of empty, headlike particles for packaging of DNA of bacteriophage lambda in vitro. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2372–2376. doi: 10.1073/pnas.71.6.2372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kaiser D., Masuda T. In vitro assembly of bacteriophage Lambda heads. Proc Natl Acad Sci U S A. 1973 Jan;70(1):260–264. doi: 10.1073/pnas.70.1.260. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Little J. W., Zimmerman S. B., Oshinsky C. K., Gellert M. Enzymatic joining of DNA strands, II. An enzyme-adenylate intermediate in the dpn-dependent DNA ligase reaction. Proc Natl Acad Sci U S A. 1967 Nov;58(5):2004–2011. doi: 10.1073/pnas.58.5.2004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mackinlay A. G., Kaiser A. D. DNA replication in head mutants of bacteriophage lambda. J Mol Biol. 1969 Feb 14;39(3):679–683. doi: 10.1016/0022-2836(69)90155-7. [DOI] [PubMed] [Google Scholar]
  13. McClure S. C., Gold M. Intermediates in the maturation of bacteriophage lambda DNA. Virology. 1973 Jul;54(1):19–27. doi: 10.1016/0042-6822(73)90110-4. [DOI] [PubMed] [Google Scholar]
  14. McClure S. C., MacHattie L., Gold M. A sedimentation analysis of DNA found in Escherichia coli infected with phage lambda mutants. Virology. 1973 Jul;54(1):1–18. doi: 10.1016/0042-6822(73)90109-8. [DOI] [PubMed] [Google Scholar]
  15. Modrich P., Lehman I. R. Enzymatic joining of polynucleotides. IX. A simple and rapid assay of polynucleotide joining (ligase) activity by measurement of circle formation from linear deoxyadenylate-deoxythymidylate copolymer. J Biol Chem. 1970 Jul 25;245(14):3626–3631. [PubMed] [Google Scholar]
  16. Mousset S., Thomas R. Ter, a function which generates the ends of the mature lambda chromosome. Nature. 1969 Jan 18;221(5177):242–244. doi: 10.1038/221242a0. [DOI] [PubMed] [Google Scholar]
  17. Murialdo H., Siminovitch L. The morphogenesis of bacteriophage lambda. IV. Identification of gene products and control of the expression of the morphogenetic information. Virology. 1972 Jun;48(3):785–823. doi: 10.1016/0042-6822(72)90162-6. [DOI] [PubMed] [Google Scholar]
  18. Sadowski P., McGeer A., Becker A. Terminal cross-linking of DNA catalyzed by an enzyme system containing DNA ligase, DNA polymerase, and exonuclease of bacteriophage T7. Can J Biochem. 1974 Jun;52(6):525–535. doi: 10.1139/o74-077. [DOI] [PubMed] [Google Scholar]
  19. Skalka A., Poonian M., Bartl P. Concatemers in DNA replication: electron microscopic studies of partially denatured intracellular lambda DNA. J Mol Biol. 1972 Mar 14;64(3):541–550. doi: 10.1016/0022-2836(72)90081-2. [DOI] [PubMed] [Google Scholar]
  20. Syvanen M. In vitro genetic recombination of bacteriophage lambda. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2496–2499. doi: 10.1073/pnas.71.6.2496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wake R. G., Kaiser A. D., Inman R. B. Isolation and structure of phage lambda head-mutant DNA. J Mol Biol. 1972 Mar 14;64(3):519–540. doi: 10.1016/0022-2836(72)90080-0. [DOI] [PubMed] [Google Scholar]
  22. Wang J. C., Kaiser A. D. Evidence that the cohesive ends of mature lambda DNA are generated by the gene A product. Nat New Biol. 1973 Jan 3;241(105):16–17. doi: 10.1038/newbio241016a0. [DOI] [PubMed] [Google Scholar]
  23. Wu R., Taylor E. Nucleotide sequence analysis of DNA. II. Complete nucleotide sequence of the cohesive ends of bacteriophage lambda DNA. J Mol Biol. 1971 May 14;57(3):491–511. doi: 10.1016/0022-2836(71)90105-7. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES