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. 1975 Mar;72(3):1082–1086. doi: 10.1073/pnas.72.3.1082

Chromatin directed transcription of 5S and tRNA genes.

W F Marzluff Jr, R C Huang
PMCID: PMC432470  PMID: 1093165

Abstract

Chromatin prepared by gentle methods from mouse myeloma cells retained its ability to synthesize RNA using bound endogenous RNA polymerase (RNA nucleotidyltransferase; nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6). The transcription resembles that observed in vivo in several respects. The low-molecular-weight RNA species 5S RNA and the 4.5S precursor to 4S RNA, are transcribed accurately and transcription is reinitiated continually in vitro. Their synthesis was not inhibited by alpha-amanitin (1 mug/ml) as was found previously for these species in isolated nuclei.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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