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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1975 Apr;72(4):1304–1308. doi: 10.1073/pnas.72.4.1304

Histone-histone propinquity by aldehyde fixation of chromatin.

R Chalkley, C Hunter
PMCID: PMC432521  PMID: 805426

Abstract

Histones have been fixed within the chromatin complex using either formaldehyde or glutaraldehyde. Evidence is presented which argues that in short time periods formaldehyde fixation leads to the formation of reversible covalent bonds between histone and DNA.On the other hand, fixation of chromatin with glutaraldehyde leads initially to the formation of polymers of F1 histone, and at a later stage of multiple small oligomers of the remaining histones. There oligomers then increase in size until they become too large to detect by polyacrylamide gel electrophoresis. Exclusive formation of histone dimers or tetramers was not observed. The simplest model for histone distribution on DNA which encompasses these observations is one in which histones are organized as a fairly extensive linear overlapping array.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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