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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1975 Apr;72(4):1368–1372. doi: 10.1073/pnas.72.4.1368

Synthesis of acetylcholine receptor by denervated rat diaphragm muscle.

J P Brockes, Z W Hall
PMCID: PMC432535  PMID: 1055412

Abstract

Acetylcholine receptor was purified by affinity chromatography from denervated rat hemidiaphragms that had been incubated in organ culture for 24 hr in medium containing [35-S] methionine. Radioactive acetylcholine receptor was identified in purified preparations by zone sedimentation in a sucrose gradient, by isoelectric focusing, and by precipitation with an antiserum to the acetylcholine receptor from electric eel. When innervated and denervated hemidiaphragms were incubated with [35-S] methionine in organ culture, and the acetylcholine receptors from each were purified separately, only the preparation from denervated muscles contained radioactive receptor as determined by zone sedimentation. We conclude that newly synthesized receptor is accumulated as a result of muscle denervation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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