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. 1975 Apr;72(4):1456–1459. doi: 10.1073/pnas.72.4.1456

Immunological relationship between acetylcholine receptor and thymus: a possible significance in myasthenia gravis.

A Aharonov, R Tarrab-Hazdai, O Abramsky, S Fuchs
PMCID: PMC432554  PMID: 1055418

Abstract

A defined immunological cross-reaction was observed between acetylcholine receptor fraction from the electric eel, Electrophorus electricus, and two calf thymus fractions. The cross-reaction was demonstrated on the cellular level by means of the lymphocyte transformation technique, and on the humoral level, by means of the microcomplement fixation assay. In the human disease myasthenia gravis both acetylcholine receptor at the neuromuscular junction and the thymus are affected, probably by an autoimmune mechanism. The immunological cross-reaction between acetylcholine receptor and thymic components may explain the association between endplate and thymus disorders in myasthenia gravis.

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Selected References

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  1. Aarli J. A., Tönder O. Antiglobulin consumption test with sera from patients with myasthenia gravis. Clin Exp Immunol. 1970 Jul;7(1):11–21. [PMC free article] [PubMed] [Google Scholar]
  2. Abramsky O., Aharonov A., Webb C., Fuchs S. Cellular immune response to acetylcholine receptor-rich fraction, in patients with myasthenia gravis. Clin Exp Immunol. 1975 Jan;19(1):11–16. [PMC free article] [PubMed] [Google Scholar]
  3. Castleman B. The pathology of the thymus gland in myasthenia gravis. Ann N Y Acad Sci. 1966 Jan 26;135(1):496–505. doi: 10.1111/j.1749-6632.1966.tb45497.x. [DOI] [PubMed] [Google Scholar]
  4. Dudai Y., Silman I., Kalderon N., Blumberg S. Purification by affinity chromatography of acetylcholinesterase from electric organ tissue of the electric eel subsequent to tryptic treatment. Biochim Biophys Acta. 1972 Apr 7;268(1):138–157. doi: 10.1016/0005-2744(72)90208-2. [DOI] [PubMed] [Google Scholar]
  5. ELLMAN G. L., COURTNEY K. D., ANDRES V., Jr, FEATHER-STONE R. M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 1961 Jul;7:88–95. doi: 10.1016/0006-2952(61)90145-9. [DOI] [PubMed] [Google Scholar]
  6. Fambrough D. M., Drachman D. B., Satyamurti S. Neuromuscular junction in myasthenia gravis: decreased acetylcholine receptors. Science. 1973 Oct 19;182(4109):293–295. doi: 10.1126/science.182.4109.293. [DOI] [PubMed] [Google Scholar]
  7. Field E. J., Bates D., Shaw D. A., Griffin S. G., Shenton B. K., Smith K. Lymphocyte sensitisation in myasthenia gravis: function of the adult thymus gland. Lancet. 1973 Sep 22;2(7830):675–676. doi: 10.1016/s0140-6736(73)92510-5. [DOI] [PubMed] [Google Scholar]
  8. Goldstein G., Hofmann W. W. Experimental myasthenia gravis. Res Publ Assoc Res Nerv Ment Dis. 1971;49:241–259. [PubMed] [Google Scholar]
  9. Goldstein G. Isolation of bovine thymin: a polypeptide hormone of the thymus. Nature. 1974 Jan 4;247(5435):11–14. doi: 10.1038/247011a0. [DOI] [PubMed] [Google Scholar]
  10. Goldstein G. The thymus and neuromuscular function. A substance in thymus which causes myositis and myasthenic neuromuscular block in guineapigs. Lancet. 1968 Jul 20;2(7560):119–122. doi: 10.1016/s0140-6736(68)90414-5. [DOI] [PubMed] [Google Scholar]
  11. Greaves M. F., Brown G. Letter: Antigenic correlations between brain and thymus. Lancet. 1974 Mar 16;1(7855):455–455. doi: 10.1016/s0140-6736(74)92409-x. [DOI] [PubMed] [Google Scholar]
  12. Gurari D., Silman I., Fuchs S. Immunochemical and enzymic properties of the electric eel acetylcholinesterase-antiacetylcholinesterase system. Eur J Biochem. 1974 Mar 15;43(1):179–187. doi: 10.1111/j.1432-1033.1974.tb03398.x. [DOI] [PubMed] [Google Scholar]
  13. Hall Z. W. Release of neurotransmitters and their interaction with receptors. Annu Rev Biochem. 1972;41:925–952. doi: 10.1146/annurev.bi.41.070172.004425. [DOI] [PubMed] [Google Scholar]
  14. Heilbronn E., Mattson C. The nicotinic cholinergic receptor protein: improved purification method, preliminary amino acid composition and observed auto-immuno response. J Neurochem. 1974 Feb;22(2):315–317. doi: 10.1111/j.1471-4159.1974.tb11597.x. [DOI] [PubMed] [Google Scholar]
  15. Hirshfeld H., Teitelbaum D., Arnon R., Sela M. Basic encephalitogenic protein: A simplified purification on sulphoethyl-sephadex. FEBS Lett. 1970 May 1;7(4):317–320. doi: 10.1016/0014-5793(70)80193-4. [DOI] [PubMed] [Google Scholar]
  16. Kalden J. R., Williamson W. G., Johnston R. J., Irvine W. J. Studies on experimental autoimmune thymitis in guinea-pigs. Clin Exp Immunol. 1969 Oct;5(4):319–340. [PMC free article] [PubMed] [Google Scholar]
  17. Kawanami S., Mori R. Experimental myasthenia in mice. The role of the thymus and lymphoid cells. Clin Exp Immunol. 1972 Dec;12(4):447–454. [PMC free article] [PubMed] [Google Scholar]
  18. Kott E., Rule A. H. Myasthenia gravis. Cellular response to basic myelin protein compared with cellular and humoral immunity to muscle antigens. Neurology. 1973 Jul;23(7):745–748. doi: 10.1212/wnl.23.7.745. [DOI] [PubMed] [Google Scholar]
  19. London Y. Ox peripheral nerve myelin membrane. Purification and partial characterization of two basic proteins. Biochim Biophys Acta. 1971 Oct 12;249(1):188–196. doi: 10.1016/0005-2736(71)90096-4. [DOI] [PubMed] [Google Scholar]
  20. Metcalf D. Delayed effect of thymectomy in adult life on immunological competence. Nature. 1965 Dec 25;208(5017):1336–1336. doi: 10.1038/2081336a0. [DOI] [PubMed] [Google Scholar]
  21. Miller J. F., Mitchell G. F., Weiss N. S. Cellular basis of the immunological defects in thymectomized mice. Nature. 1967 Jun 3;214(5092):992–997. doi: 10.1038/214992a0. [DOI] [PubMed] [Google Scholar]
  22. Mori R., Kawanami S. Destruction of cultured thymus cells by autologous lymphocytes from a patient with myasthenia gravis. Lancet. 1973 Jan 27;1(7796):210–210. doi: 10.1016/s0140-6736(73)90051-2. [DOI] [PubMed] [Google Scholar]
  23. Olsen R. W., Meunier J. C., Changeux J. P. Progress in the purification of the cholinergic receptor protein from Electrophorus electricus by affinity chromatography. FEBS Lett. 1972 Nov 15;28(1):96–100. doi: 10.1016/0014-5793(72)80686-0. [DOI] [PubMed] [Google Scholar]
  24. Papatestas A. E., Alpert L. I., Osserman K. E., Osserman R. S., Kark A. E. Studies in myasthenia gravis: effects of thymectomy. Results on 185 patients with nonthymomatous and thymomatous myasthenia gravis, 1941-1969. Am J Med. 1971 Apr;50(4):465–474. doi: 10.1016/0002-9343(71)90336-6. [DOI] [PubMed] [Google Scholar]
  25. Patrick J., Lindstrom J. Autoimmune response to acetylcholine receptor. Science. 1973 May 25;180(4088):871–872. doi: 10.1126/science.180.4088.871. [DOI] [PubMed] [Google Scholar]
  26. Rule A. H., Kornfeld P. Studies in myasthenia gravis: biologic aspects. Mt Sinai J Med. 1971 Nov-Dec;38(6):538–572. [PubMed] [Google Scholar]
  27. Schlesinger M. Antigens of the thymus. Prog Allergy. 1972;16:214–299. doi: 10.1159/000313173. [DOI] [PubMed] [Google Scholar]
  28. Sugiyama H., Benda P., Meunier J. C., Changeux J. P. Immunological characterisation of the cholinergic receptor protein from Electrophorus electricus. FEBS Lett. 1973 Sep 1;35(1):124–128. doi: 10.1016/0014-5793(73)80592-7. [DOI] [PubMed] [Google Scholar]
  29. Tarrab R., Sulica A., Haimovich J., Sela M. Cell proliferation and antibody production in the secondary response in vitro towards a defined hapten. Eur J Immunol. 1971 Aug;1(4):231–236. doi: 10.1002/eji.1830010403. [DOI] [PubMed] [Google Scholar]
  30. Taylor R. B. Decay of immunological responsiveness after thymectomy in adult life. Nature. 1965 Dec 25;208(5017):1334–1335. doi: 10.1038/2081334a0. [DOI] [PubMed] [Google Scholar]
  31. Trainin N. Thymic hormones and the immune response. Physiol Rev. 1974 Apr;54(2):272–315. doi: 10.1152/physrev.1974.54.2.272. [DOI] [PubMed] [Google Scholar]
  32. van der Geld H. W., Strauss A. J. Myasthenia gravis. Immunological relationship between striated muscle and thymus. Lancet. 1966 Jan 8;1(7428):57–60. doi: 10.1016/s0140-6736(66)92356-7. [DOI] [PubMed] [Google Scholar]

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