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. 1975 Apr;72(4):1505–1509. doi: 10.1073/pnas.72.4.1505

Affinity labeling of the ribosomal decoding site with an AUG-substrate analog.

O Pongs, E Lanka
PMCID: PMC432565  PMID: 1093183

Abstract

The trinucleotide AUG was condensed at the 5'-end with N-bromoacetyl-p-aminophenylphosphate. This bromoactylated AUG analog reacted irreversibly with the mRNA binding site of Escherichia coli 70S ribosomes. After reaction of 70S ribosomes with the AUG analog, labeled 30S subunits could be isolated that were programmed for initiation-factor-dependent binding of fMet-tRNAfMet. This shows that this AUG-affinity label reacted in the decoding site for fMet-tRNAfMet. By combination of sodium dodecyl sulfate-, Sarkosyl-, and ureapolyacrylamide gel electrophoresis the AUG-affinity label was found to be irreversibly bound to ribosomal proteins S4, the ram gene product, and S18.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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