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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1975 Jun;72(6):2099–2103. doi: 10.1073/pnas.72.6.2099

DNA synthesis involving a complexes form of DNA polymerase I in extracts of Escherichia coli.

R W Hendler, M Pereira, R Scharff
PMCID: PMC432703  PMID: 1094453

Abstract

DNA polymerase I (EC 2.7.7.7; deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase) has been recovered as a complex of about 390,000 molecular weight. The complex displays an ATP-stimulated DNA-synthesizing activity that prefers native to heat-denatured DNA. Genetic evidence indicates that the recBC enzyme is associated with the polymerase in the complex. Preliminary evidence for complexes involving DNA polymerases II and III is also presented.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barbour S. D., Clark A. J. Biochemical and genetic studies of recombination proficiency in Escherichia coli. I. Enzymatic activity associated with recB+ and recC+ genes. Proc Natl Acad Sci U S A. 1970 Apr;65(4):955–961. doi: 10.1073/pnas.65.4.955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gefter M. L., Hirota Y., Kornberg T., Wechsler J. A., Barnoux C. Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3150–3153. doi: 10.1073/pnas.68.12.3150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Goldmark P. J., Linn S. An endonuclease activity from Escherichia coli absent from certain rec- strains. Proc Natl Acad Sci U S A. 1970 Sep;67(1):434–441. doi: 10.1073/pnas.67.1.434. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Goldmark P. J., Linn S. Purification and properties of the recBC DNase of Escherichia coli K-12. J Biol Chem. 1972 Mar 25;247(6):1849–1860. [PubMed] [Google Scholar]
  5. Hendler R. W., Burgess A. H., Scharff R. Respiration and protein synthesis in Escherichia coli membrane-envelope fragments. I. Oxidative activities with soluble substrates. J Cell Biol. 1969 Sep;42(3):715–732. doi: 10.1083/jcb.42.3.715. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Howard-Flanders P. DNA repair and recombination. Br Med Bull. 1973 Sep;29(3):226–235. doi: 10.1093/oxfordjournals.bmb.a071012. [DOI] [PubMed] [Google Scholar]
  7. Jovin T. M., Englund P. T., Bertsch L. L. Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase. J Biol Chem. 1969 Jun 10;244(11):2996–3008. [PubMed] [Google Scholar]
  8. Karu A. E., MacKay V., Goldmark P. J., Linn S. The recBC deoxyribonuclease of Escherichia coli K-12. Substrate specificity and reaction intermediates. J Biol Chem. 1973 Jul 25;248(14):4874–4884. [PubMed] [Google Scholar]
  9. Klenow H., Henningsen I. Effect of monovalent cations on the activity of the DNA polymerase of Escherichia coli B. Eur J Biochem. 1969 May 1;9(1):133–141. doi: 10.1111/j.1432-1033.1969.tb00586.x. [DOI] [PubMed] [Google Scholar]
  10. Kornberg A. Active center of DNA polymerase. Science. 1969 Mar 28;163(3874):1410–1418. doi: 10.1126/science.163.3874.1410. [DOI] [PubMed] [Google Scholar]
  11. Kornberg T., Gefter M. L. DNA synthesis in cell-free extracts of a DNA polymerase-defective mutant. Biochem Biophys Res Commun. 1970 Sep 30;40(6):1348–1355. doi: 10.1016/0006-291x(70)90014-8. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. MacKay V., Linn S. The mechanism of degradation of duplex deoxyribonucleic acid by the recBC enzyme of Escherichia coli K-12. J Biol Chem. 1974 Jul 10;249(13):4286–4294. [PubMed] [Google Scholar]
  14. Momparler R. L. Effect of cytosine arabinoside 5'-triphosphate on mammalian DNA polymerase. Biochem Biophys Res Commun. 1969 Feb 21;34(4):464–471. doi: 10.1016/0006-291x(69)90405-7. [DOI] [PubMed] [Google Scholar]
  15. Moses R. E., Campbell J. L., Fleischman R. A., Frenkel G. D., Mulcahy H. L., Shizuya H., Richardson C. C. Enzymatic mechanisms of DNA replication in Escherichia coli. Fed Proc. 1972 Sep-Oct;31(5):1415–1421. [PubMed] [Google Scholar]
  16. Nobrega F. G., Rola F. H., Pasetto-Nobrega M., Oishi M. Adenosine triphosphatase associated with adenosine triphosphate-dependent deoxyribonuclease (recB-recC enzyme-E. coli-ATP to phosphodiester hydrolysis ratio-DNA-dependent ATPase activity). Proc Natl Acad Sci U S A. 1972 Jan;69(1):15–19. doi: 10.1073/pnas.69.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Oishi M. An ATP-dependent deoxyribonuclease from Escherichia coli with a possible role in genetic recombination. Proc Natl Acad Sci U S A. 1969 Dec;64(4):1292–1299. doi: 10.1073/pnas.64.4.1292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. RICHARDSON C. C., SCHILDKRAUT C. L., APOSHIAN H. V., KORNBERG A. ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI. J Biol Chem. 1964 Jan;239:222–232. [PubMed] [Google Scholar]
  19. Rama Reddy G. V., Goulian M., Hendler S. S. Inhibition of E. coli DNA polymerase II by ara-CTP. Nat New Biol. 1971 Dec 29;234(52):286–288. doi: 10.1038/newbio234286a0. [DOI] [PubMed] [Google Scholar]
  20. Scharff R., Hendler R. W., Nanninga N., Burgess A. H. Respiration and protein synthesis in Escherichia coli membrane-envelope fragments. IV. Chemical and cytological characterization and biosynthetic capabilities of fragments obtained by mild procedures. J Cell Biol. 1972 Apr;53(1):1–23. doi: 10.1083/jcb.53.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tait R. C., Harris A. L., Smith D. W. DNA repair in Escherichia coli mutants deficient in DNA polymerases I, II and-or 3. Proc Natl Acad Sci U S A. 1974 Mar;71(3):675–679. doi: 10.1073/pnas.71.3.675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wickner W., Kornberg A. A holoenzyme form of deoxyribonucleic acid polymerase III. Isolation and properties. J Biol Chem. 1974 Oct 10;249(19):6244–6249. [PubMed] [Google Scholar]
  23. Wickner W., Schekman R., Geider K., Kornberg A. A new form of DNA polymerase 3 and a copolymerase replicate a long, single-stranded primer-template. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1764–1767. doi: 10.1073/pnas.70.6.1764. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wright M., Buttin G., Hurwitz J. The isolation and characterization from Escherichia coli of an adenosine triphosphate-dependent deoxyribonuclease directed by rec B, C genes. J Biol Chem. 1971 Nov;246(21):6543–6555. [PubMed] [Google Scholar]

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