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. 1975 Jun;72(6):2289–2293. doi: 10.1073/pnas.72.6.2289

Regulation of protein synthesis at the translational level in neuroblastoma cells.

F Zucco, M Persico, A Felsani, S Metafora, G Augusti-Tocco
PMCID: PMC432743  PMID: 1056031

Abstract

Protein synthesis in reuroblastoma cells has been studied in a cell-free system. The activity of lysates from cells grown insuspension and monolayer has been compared. A higher level of activity has been found in monolayer cells. The activity of some components of the lysate that are involved in protein synthesis has been analyzed.The data suggest that the controlling step of protein synthesis in this system might be in the initiation process. The correlation between activation of protein synthesis and neurite outgrowth in monolayer cultures is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ACS G., NEIDLE A., SCHNEIDERMAN N. The effect of fatty acids on amino acid incorporation. Biochim Biophys Acta. 1962 Jan 29;56:373–374. doi: 10.1016/0006-3002(62)90582-6. [DOI] [PubMed] [Google Scholar]
  2. Andrews T. M., Tata J. R. Protein synthesis by membrane-bound and free ribosomes of secretory and non-secretory tissues. Biochem J. 1971 Feb;121(4):683–694. doi: 10.1042/bj1210683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Augusti-Tocco G., Casola L., Romano M. RNA metabolism in neuroblastoma cultures. II. Synthesis of non-ribosomal RNA. Cell Differ. 1974 Dec;3(5):313–320. doi: 10.1016/0045-6039(74)90022-0. [DOI] [PubMed] [Google Scholar]
  4. Augusti-Tocco G., Sato G. Establishment of functional clonal lines of neurons from mouse neuroblastoma. Proc Natl Acad Sci U S A. 1969 Sep;64(1):311–315. doi: 10.1073/pnas.64.1.311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Casola L., Romano M., Di Matteo G. RNA metabolism in neuroblastoma cultures. I. Ribosomal RNA. Dev Biol. 1974 Dec;41(2):371–379. doi: 10.1016/0012-1606(74)90313-3. [DOI] [PubMed] [Google Scholar]
  6. Dunn A. J. The limiting factors of a cell-free protein-synthesizing system from rat brain. Biochem J. 1970 Jan;116(1):135–145. doi: 10.1042/bj1160135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fan H., Penman S. Regulation of protein synthesis in mammalian cells. II. Inhibition of protein synthesis at the level of initiation during mitosis. J Mol Biol. 1970 Jun 28;50(3):655–670. doi: 10.1016/0022-2836(70)90091-4. [DOI] [PubMed] [Google Scholar]
  8. Felicetti L., Metafora S., Gambino R., Di Matteo G. Characterization and activity of the elongation factors T1 and T2 in the unfertilized egg and in the early development of sea urchin. Cell Differ. 1972 Dec;1(5):265–277. doi: 10.1016/0045-6039(72)90002-4. [DOI] [PubMed] [Google Scholar]
  9. Glick M. C., Kimhi Y., Littauer U. Z. Glycopeptides from surface membranes of neuroblastoma cells. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1682–1687. doi: 10.1073/pnas.70.6.1682. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Harris A. J., Dennis M. J. Acetylcholine sensitivity and distribution on mouse neuroblastoma cells. Science. 1970 Feb 27;167(3922):1253–1255. doi: 10.1126/science.167.3922.1253. [DOI] [PubMed] [Google Scholar]
  11. KORNER A. Studies on incorporation of amino acids into protein in isolated rat-liver ribosomes. Biochem J. 1961 Oct;81:168–178. doi: 10.1042/bj0810168. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. McCormick W., Penman S. Regulation of protein synthesis in HeLa cells: translation at elevated temperatures. J Mol Biol. 1969 Jan;39(2):315–333. doi: 10.1016/0022-2836(69)90320-9. [DOI] [PubMed] [Google Scholar]
  14. Metafora S., Felicetti L., Gambino R. The mechanism of protein synthesis activation after fertilization of sea urchin eggs. Proc Natl Acad Sci U S A. 1971 Mar;68(3):600–604. doi: 10.1073/pnas.68.3.600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Metafora S., Terada M., Dow L. W., Marks P. A., Bank A. Increased efficiency of exogenous messenger RNA translation in a Krebs ascites cell lysate. Proc Natl Acad Sci U S A. 1972 May;69(5):1299–1303. doi: 10.1073/pnas.69.5.1299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Murthy M. R. Free and membrane-bound ribosomes of rat cerebral cortex. Protein synthesis in vivo and in vitro. J Biol Chem. 1972 Mar 25;247(6):1936–1943. [PubMed] [Google Scholar]
  17. Palmiter R. D. Regulation of protein synthesis in chick oviduct. II. Modulation of polypeptide elongation and initiation rates by estrogen and progesterone. J Biol Chem. 1972 Nov 10;247(21):6770–6780. [PubMed] [Google Scholar]
  18. Schochetman G., Perry R. P. Characterization of the messenger RNA released from L cell polyribosomes as a result of temperature shock. J Mol Biol. 1972 Feb 14;63(3):577–590. doi: 10.1016/0022-2836(72)90449-4. [DOI] [PubMed] [Google Scholar]
  19. Schubert D., Humphreys S., Baroni C., Cohn M. In vitro differentiation of a mouse neuroblastoma. Proc Natl Acad Sci U S A. 1969 Sep;64(1):316–323. doi: 10.1073/pnas.64.1.316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schubert D., Jacob F. 5-bromodeoxyuridine-induced differentiation of a neuroblastoma. Proc Natl Acad Sci U S A. 1970 Sep;67(1):247–254. doi: 10.1073/pnas.67.1.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Seeds N. W., Gilman A. G., Amano T., Nirenberg M. W. Regulation of axon formation by clonal lines of a neural tumor. Proc Natl Acad Sci U S A. 1970 May;66(1):160–167. doi: 10.1073/pnas.66.1.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Vaughan M. H., Jr, Pawlowski P. J., Forchhammer J. Regulation of protein synthesis initiation in HeLa cells deprived of single essential amino acids. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2057–2061. doi: 10.1073/pnas.68.9.2057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Zomzely-Neurath C., York C., Moore B. W. Synthesis of a brain-specific protein (S100 protein) in a homologous cell-free system programmed with cerebral polysomal messenger RNA. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2326–2330. doi: 10.1073/pnas.69.8.2326. [DOI] [PMC free article] [PubMed] [Google Scholar]

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