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. 1975 Jul;72(7):2559–2562. doi: 10.1073/pnas.72.7.2559

3'-Terminal nucletide sequence (n equals 361) of bacteriophage MS2 RNA.

A Vandenberghe, W Min Jou, W Fiers
PMCID: PMC432808  PMID: 809766

Abstract

32P-Labeled MS2 RNA was partially digested with ribonuclease T1 (guanyloribonuclease; ribonucleate 3'-guanylo-oligonucleotidohydrolase; EC 3.1.4.8) or with epilson-carboxymethyl-lysine-41 pancreatic ribonuclease A (ribonucleate 3'-pyrimidino-oligonucleotidohydrolase; EC 3.1.4.22). A series of overlapping fragments was obtained which allowed the reconstruction of a 361-nucleotide-long 3'-terminal sequence. A unique reading frame could be deduced, which indicated that the replicase gene ends with a U-A-G termination signal and is followed by a 174-nucleotide-long untranslated segment.

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Selected References

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  1. Capecchi M. R., Klein H. A. Release factors mediating termination of complete proteins. Nature. 1970 Jun 13;226(5250):1029–1033. doi: 10.1038/2261029a0. [DOI] [PubMed] [Google Scholar]
  2. Contreras R., Fiers W. A new method for partial digestion useful for sequence analysis of polynucleotides. FEBS Lett. 1971 Sep 1;16(4):281–283. doi: 10.1016/0014-5793(71)80370-8. [DOI] [PubMed] [Google Scholar]
  3. Contreras R., Vandenberghe A., Jou W. Min, De Wachter R., Fiers W. Studies on the bacteriophage MS2 nucleotide sequence of a 3'-terminal fragment (n = 104). FEBS Lett. 1971 Oct 15;18(1):141–144. doi: 10.1016/0014-5793(71)80430-1. [DOI] [PubMed] [Google Scholar]
  4. Contreras R., Ysebaert M., Jou W. M., Fiers W. Bacteriophage Ms2 RNA: nucleotide sequence of the end of the a protein gene and the intercistronic region. Nat New Biol. 1973 Jan 24;241(108):99–101. doi: 10.1038/newbio241099a0. [DOI] [PubMed] [Google Scholar]
  5. Cory S., Adams J. M., Spahr P. F., Rensing U. Sequence of 51 nucleotides at the 3'-end of R17 bacteriophage RNA. J Mol Biol. 1972 Jan 14;63(1):41–56. doi: 10.1016/0022-2836(72)90520-7. [DOI] [PubMed] [Google Scholar]
  6. De Wachter R., Fiers W. Studies on the bacteriophage MS2. IV. The 3'-OH terminal undecanucleotide sequence of the viral RNA chain. J Mol Biol. 1967 Dec 28;30(3):507–527. [PubMed] [Google Scholar]
  7. De Wachter R., Vandenberghe A., Merregaert J., Contreras R., Fiers W. The leader sequence from the 5'-terminus to the A-protein initiation codon in MS2-virus RNA. Proc Natl Acad Sci U S A. 1971 Mar;68(3):585–589. doi: 10.1073/pnas.68.3.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fiers W., Contreras R., De Wachter R., Haegeman G., Merregaert J., Jou W. M., Vandenberghe A. Recent progress in the sequence determination of bacteriophage MS2 RNA. Biochimie. 1971;53(4):495–506. doi: 10.1016/s0300-9084(71)80167-0. [DOI] [PubMed] [Google Scholar]
  9. Haegeman G., Fiers W. Studies on the bacteriophage MS2. An internal nucleotide fragment resembling some ribosomal binding sites. Eur J Biochem. 1973 Jul 2;36(1):135–143. doi: 10.1111/j.1432-1033.1973.tb02893.x. [DOI] [PubMed] [Google Scholar]
  10. Haegeman G., Jou W. M., Fiers W. Studies on the bacteriophage MS2. IX. The heptanucleotide sequences present in the pancreatic ribonuclease digest of the viral RNA. J Mol Biol. 1971 May 14;57(3):597–613. doi: 10.1016/0022-2836(71)90112-4. [DOI] [PubMed] [Google Scholar]
  11. Jou W. M., Contreras R., Fiers W. The 3'-terminal nucleotide sequence (n = 16) of bacteriophage MS2 RNA. FEBS Lett. 1970 Aug 17;9(4):222–224. doi: 10.1016/0014-5793(70)80360-x. [DOI] [PubMed] [Google Scholar]
  12. Konigsberg W., Maita T., Katze J., Weber K. Amino-acid sequence of the "Qbeta" coat protein. Nature. 1970 Jul 18;227(5255):271–273. doi: 10.1038/227271a0. [DOI] [PubMed] [Google Scholar]
  13. Min Jou W., Haegeman G., Ysebaert M., Fiers W. Nucleotide sequence of the gene coding for the bacteriophage MS2 coat protein. Nature. 1972 May 12;237(5350):82–88. doi: 10.1038/237082a0. [DOI] [PubMed] [Google Scholar]
  14. Porter A. G., Hindley J., Billeter M. A. A sequence of 83 nucleotides containing the replicase cistron ribosome binding site of phage Qbeta RNA. Eur J Biochem. 1974 Jan 16;41(2):413–420. doi: 10.1111/j.1432-1033.1974.tb03282.x. [DOI] [PubMed] [Google Scholar]
  15. Remaut E., Fiers W. Studies on the bacteriophage MS2. XVI. The termination signal of the A protein cistron. J Mol Biol. 1972 Nov 14;71(2):243–261. doi: 10.1016/0022-2836(72)90349-x. [DOI] [PubMed] [Google Scholar]
  16. Sanger F., Brownlee G. G., Barrell B. G. A two-dimensional fractionation procedure for radioactive nucleotides. J Mol Biol. 1965 Sep;13(2):373–398. doi: 10.1016/s0022-2836(65)80104-8. [DOI] [PubMed] [Google Scholar]
  17. Tinoco I., Jr, Borer P. N., Dengler B., Levin M. D., Uhlenbeck O. C., Crothers D. M., Bralla J. Improved estimation of secondary structure in ribonucleic acids. Nat New Biol. 1973 Nov 14;246(150):40–41. doi: 10.1038/newbio246040a0. [DOI] [PubMed] [Google Scholar]
  18. Weissmann C., Billeter M. A., Goodman H. M., Hindley J., Weber H. Structure and function of phage RNA. Annu Rev Biochem. 1973;42:303–328. doi: 10.1146/annurev.bi.42.070173.001511. [DOI] [PubMed] [Google Scholar]
  19. de Wachter R., Fiers W. Preparative two-dimensional polyacrylamide gel electrophoresis of 32 P-labeled RNA. Anal Biochem. 1972 Sep;49(1):184–197. doi: 10.1016/0003-2697(72)90257-6. [DOI] [PubMed] [Google Scholar]
  20. de Wachter R., Merregaert J., Vandenberghe A., Contreras R., Fiers W. Studies on the bacteriophage MS2. The untranslated 5'-terminal nucleotide sequence preceding the first cistron. Eur J Biochem. 1971 Oct 14;22(3):400–414. doi: 10.1111/j.1432-1033.1971.tb01558.x. [DOI] [PubMed] [Google Scholar]

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