Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1975 Aug;72(8):2910–2914. doi: 10.1073/pnas.72.8.2910

Presence of polyriboadenylate sequences in pulse-labeled RNA of Escherichia coli.

P R Srinivasan, M Ramanarayanan, E Rabbani
PMCID: PMC432888  PMID: 1103127

Abstract

Pulse-labeled RNA isolated from E. coli cells grown on limiting phosphate medium and phosphate-containing medium was analyzed by oligo(dT)-cellulose chromatography and by Millipore binding assay for polyriboadenylate-containing RNA. Whereas poly(A)-containing RNA amounted to as much as 15% of the total pulse-labeled RNA from cells grown on limiting phosphate medium, pulse-labeled RNA from cells grown on phosphate medium gave values around 1.5%. Steady-state labeled RNA from cells grown on limiting phosphate medium contained 1.2% poly(A) RNA. The addition of poly(A) sequences appears to be post-transcriptional. These results strongly favor the view that bacterial mRNAs may contain poly(A) stretches.

Full text

PDF
2912

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARBER W. HOST SPECIFICITY OF DNA PRODUCED BY ESCHERICHIA COLI V . THE ROLE OF METHIONINE IN THE PRODUCTION OF HOST SPECIFICITY. J Mol Biol. 1965 Feb;11:247–256. doi: 10.1016/s0022-2836(65)80055-9. [DOI] [PubMed] [Google Scholar]
  2. Armstrong J. A., Edmonds M., Nakazato H., Phillips B. A., Vaughn M. H. Polyadenylic acid sequences in the virion RNA of poliovirus and Eastern Equine Encephalitis virus. Science. 1972 May 5;176(4034):526–528. doi: 10.1126/science.176.4034.526. [DOI] [PubMed] [Google Scholar]
  3. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Darnell J. E., Jelinek W. R., Molloy G. R. Biogenesis of mRNA: genetic regulation in mammalian cells. Science. 1973 Sep 28;181(4106):1215–1221. doi: 10.1126/science.181.4106.1215. [DOI] [PubMed] [Google Scholar]
  5. Darnell J. E., Philipson L., Wall R., Adesnik M. Polyadenylic acid sequences: role in conversion of nuclear RNA into messenger RNA. Science. 1971 Oct 29;174(4008):507–510. doi: 10.1126/science.174.4008.507. [DOI] [PubMed] [Google Scholar]
  6. Darnell J. E., Wall R., Tushinski R. J. An adenylic acid-rich sequence in messenger RNA of HeLa cells and its possible relationship to reiterated sites in DNA. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1321–1325. doi: 10.1073/pnas.68.6.1321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. ECHOLS H., GAREN A., GAREN S., TORRIANI A. Genetic control of repression of alkaline phosphatase in E. coli. J Mol Biol. 1961 Aug;3:425–438. doi: 10.1016/s0022-2836(61)80055-7. [DOI] [PubMed] [Google Scholar]
  8. Edmonds M., Caramela M. G. The isolation and characterization of adenosine monophosphate-rich polynucleotides synthesized by Ehrlich ascites cells. J Biol Chem. 1969 Mar 10;244(5):1314–1324. [PubMed] [Google Scholar]
  9. Edmonds M., Kopp D. W. The occurrence of polyadenylate sequences in bacteria and yeast. Biochem Biophys Res Commun. 1970 Dec 24;41(6):1531–1537. doi: 10.1016/0006-291x(70)90561-9. [DOI] [PubMed] [Google Scholar]
  10. Edmonds M., Vaughan M. H., Jr, Nakazato H. Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1336–1340. doi: 10.1073/pnas.68.6.1336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kramer R. A., Rosenberg M., Steitz J. A. Nucleotide sequences of the 5' and 3' termini of bacteriophage T7 early messenger RNAs synthesized in vivo: evidence for sequence specificity in RNA processing. J Mol Biol. 1974 Nov 15;89(4):767–776. doi: 10.1016/0022-2836(74)90051-5. [DOI] [PubMed] [Google Scholar]
  12. Lai M. M., Duesberg P. H. Adenylic acid-rich sequence in RNAs of Rous sarcoma virus and Rauscher mouse leukaemia virus. Nature. 1972 Feb 18;235(5338):383–386. doi: 10.1038/235383c0. [DOI] [PubMed] [Google Scholar]
  13. Lee S. Y., Mendecki J., Brawerman G. A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1331–1335. doi: 10.1073/pnas.68.6.1331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lim L., Canellakis E. S. Adenine-rich polymer associated with rabbit reticulocyte messenger RNA. Nature. 1970 Aug 15;227(5259):710–712. doi: 10.1038/227710a0. [DOI] [PubMed] [Google Scholar]
  15. Mendecki J., Lee S. Y., Brawerman G. Characteristics of the polyadenylic acid segment associated with messenger ribonucleic acid in mouse sarcoma 180 ascites cells. Biochemistry. 1972 Feb 29;11(5):792–798. doi: 10.1021/bi00755a018. [DOI] [PubMed] [Google Scholar]
  16. Milanino R., Chargaff E. A purine polyribonucleotide synthetase from Escherichia coli. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2558–2562. doi: 10.1073/pnas.70.9.2558. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Modak M. J., Srinivasan P. R. Purification and properties of a ribonucleic acid primer-independent polyriboadenylate polymerase from Escherichia coli. J Biol Chem. 1973 Oct 10;248(19):6904–6910. [PubMed] [Google Scholar]
  18. Perry R. P., Kelley D. E., LaTorre J. Lack of polyadenylic acid sequences in the messenger RNA of E. coli. Biochem Biophys Res Commun. 1972 Sep 26;48(6):1593–1600. doi: 10.1016/0006-291x(72)90896-0. [DOI] [PubMed] [Google Scholar]
  19. Philipson L., Wall R., Glickman G., Darnell J. E. Addition of polyadenylate sequences to virus-specific RNA during adenovirus replication. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2806–2809. doi: 10.1073/pnas.68.11.2806. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rosenfeld M. G., Abrass I. B., Perkins L. A. Cleavage of the polyadenylate-rich region of polyadenylate-rich RNA. Biochem Biophys Res Commun. 1972 Oct 6;49(1):230–238. doi: 10.1016/0006-291x(72)90034-4. [DOI] [PubMed] [Google Scholar]
  21. Sheldon R., Kates J., Kelley D. E., Perry R. P. Polyadenylic acid sequences on 3' termini of vaccinia messenger ribonucleic acid and mammalian nuclear and messenger ribonucleic acid. Biochemistry. 1972 Sep 26;11(20):3829–3834. doi: 10.1021/bi00770a023. [DOI] [PubMed] [Google Scholar]
  22. Weinberg R. A., Ben-Ishai Z., Newbold J. E. Poly A associated with SV40 messenger RNA. Nat New Biol. 1972 Jul 26;238(82):111–113. doi: 10.1038/newbio238111a0. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES