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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1975 Sep;72(9):3364–3368. doi: 10.1073/pnas.72.9.3364

Effect of estrogen on gene expression: purification of vitellogenin messenger RNA.

W Wetekam, K P Mullinix, R G Deeley, H M Kronenberg, J D Eldridge, M Meyers, R F Goldberger
PMCID: PMC432993  PMID: 1059123

Abstract

We report initial studies on estrogen-mediated regulation of egg yolk protein synthesis in the rooster. Egg yolk proteins are normally synthesized as a large precursor, vitellogenin, in the liver of the laying hen; roosters synthesize vitellogenin only when treated with estrogen. Polysomal RNA from the liver of estrogen-treated roosters was translated in a reticulocyte cell-free system, and the newly synthesized proteins were identified by a highly specific and sensitive indirect immunoprecipitation reaction. The messenger RNA that specifies vitellogenin has been purified more than 800-fold from rooster liver polysomal RNA by a combination of methods, including immunoprecipitation of polysomes and chromatography of RNA on poly(U)-Sepharose.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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