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. 1973 Jan;70(1):115–119. doi: 10.1073/pnas.70.1.115

Stability of Cytoplasmic Messenger RNA in HeLa Cells

William Murphy 1, Giuseppe Attardi 1
PMCID: PMC433196  PMID: 4509644

Abstract

The metabolic stability of pulse-labeled or long-term labeled messenger RNA (mRNA) from cytoplasmic free polysomes was measured in HeLa cells, with chase conditions that do not involve inhibitors of RNA synthesis, and chromatography on benzoylated-DEAE-cellulose or poly(T)-cellulose for isolation of mRNA. For these studies, a new chase technique was developed that allows analysis of the stability of mRNA labeled during a short [3H]uridine pulse. Pulse-labeled and long-term labeled mRNA were found to decay with an estimated average half-life of about 2 and 3 days, respectively, much longer than hitherto assumed.

Keywords: actinomycin D, benzoylated-DEAE-cellulose, poly(T)-cellulose, nucleoside triphosphate pools, cold chase

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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