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. 1973 Jan;70(1):200–204. doi: 10.1073/pnas.70.1.200

Cleavage of Adenovirus Type 2 DNA into Six Unique Fragments by Endonuclease R·RI

Ulf Pettersson 1, Carel Mulder 1, Hajo Delius 1, Phillip A Sharp 1
PMCID: PMC433215  PMID: 4567334

Abstract

The DNA of adenovirus type 2 was cleaved by restriction endonuclease R·RI into six fragments. These fragments were separated by electrophoresis on composite agarose-polyacrylamide gels. Their molecular weights ranged from 1.1 × 106 to 13.6 × 106, as measured by electron microscopy. Each fragment represented a unique segment of adenovirus type 2 DNA since: (i) the fragments were obtained in equimolar amounts; (ii) the sum of their molecular weights was equal to the molecular weight of complete adenovirus DNA; and (iii) each fragment exhibited a rate of renaturation that was inversely proportional to its size.

Keywords: restriction enzyme, electron microscopy, DNA gel electrophoresis, DNA sequence complexity

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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