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. 1973 Feb;70(2):543–547. doi: 10.1073/pnas.70.2.543

Microinjection of Rabbit Hemoglobin Messenger RNA into Amphibian Oocytes and Embryos

J Brachet *, G Huez †, E Hubert *
PMCID: PMC433302  PMID: 4510295

Abstract

After microinjection of hemoglobin mRNA from rabbit reticulocytes (9S mRNA) into Xenopus laevis oocytes, the cytological structure remains normal. The peroxidase reaction does not show an accumulation of synthesized hemoglobin in the germinal vesicle. Fertilized Pleurodeles Waltlii eggs synthesize rabbit hemoglobin after injection of 9S mRNA; hemoglobin synthesis decreases as development progresses. Micromeres and macromeres synthesize equal amounts of hemoglobin, despite the fact that the micromeres contain many more ribosomes. Descendants of injected blastomeres divide more slowly and do not differentiate; they ultimately undergo cytolysis.

Keywords: 9S RNA, globin mRNA, Xenopus oocytes, Pleurodeles embryos

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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