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. 1973 Mar;70(3):924–928. doi: 10.1073/pnas.70.3.924

A Protein of Molecular Weight 78,000 Bound to the Polyadenylate Region of Eukaryotic Messenger RNAs

Günter Blobel 1
PMCID: PMC433389  PMID: 4515002

Abstract

Two distinct proteins were found to be tightly bound to the heterogeneous messenger RNAs associated with polysomes in mouse L cells and rat hepatocytes. The molecular weight (78,000) of the larger of these two proteins is identical to that of the protein previously found associated with rabbit globin messenger RNA. This protein is shown to be bound to the adenylate-rich region of messenger RNA. The molecular weight of the smaller protein associated with messenger RNAs from hepatic and L-cell polysomes is similar to that found in globin messenger ribonucleoprotein.

Keywords: L cells, rat hepatocytes, rabbit reticulocytes, polysomes

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adesnik M., Darnell J. E. Biogenesis and characterization of histone messenger RNA in HeLa cells. J Mol Biol. 1972 Jun 28;67(3):397–406. doi: 10.1016/0022-2836(72)90458-5. [DOI] [PubMed] [Google Scholar]
  2. Baltimore D., Huang A. S. Interaction of HeLa cell proteins with RNA. J Mol Biol. 1970 Feb 14;47(3):263–273. doi: 10.1016/0022-2836(70)90301-3. [DOI] [PubMed] [Google Scholar]
  3. Blobel G. Isolation of a 5S RNA-protein complex from mammalian ribosomes. Proc Natl Acad Sci U S A. 1971 Aug;68(8):1881–1885. doi: 10.1073/pnas.68.8.1881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blobel G. Protein tightly bound to globin mRNA. Biochem Biophys Res Commun. 1972 Apr 14;47(1):88–95. doi: 10.1016/s0006-291x(72)80014-7. [DOI] [PubMed] [Google Scholar]
  5. Blobel G. Release, identification, and isolation of messenger RNA from mammalian ribosomes. Proc Natl Acad Sci U S A. 1971 Apr;68(4):832–835. doi: 10.1073/pnas.68.4.832. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Blobel G., Sabatini D. Dissociation of mammalian polyribosomes into subunits by puromycin. Proc Natl Acad Sci U S A. 1971 Feb;68(2):390–394. doi: 10.1073/pnas.68.2.390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Darnell J. E., Wall R., Tushinski R. J. An adenylic acid-rich sequence in messenger RNA of HeLa cells and its possible relationship to reiterated sites in DNA. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1321–1325. doi: 10.1073/pnas.68.6.1321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Edmonds M., Vaughan M. H., Jr, Nakazato H. Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1336–1340. doi: 10.1073/pnas.68.6.1336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Greenman D. L., Huang R. C., Smith M., Furrow L. M. Thin-layer separation and quantitative elution of nucleosides and nucleotides. Anal Biochem. 1969 Oct 1;31(1):348–359. doi: 10.1016/0003-2697(69)90275-9. [DOI] [PubMed] [Google Scholar]
  10. Kwan S. W., Brawerman G. A particle associated with the polyadenylate segment in mammalian messenger RNA. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3247–3250. doi: 10.1073/pnas.69.11.3247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lee S. Y., Mendecki J., Brawerman G. A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1331–1335. doi: 10.1073/pnas.68.6.1331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lim L., Canellakis E. S. Adenine-rich polymer associated with rabbit reticulocyte messenger RNA. Nature. 1970 Aug 15;227(5259):710–712. doi: 10.1038/227710a0. [DOI] [PubMed] [Google Scholar]
  13. Sheldon R., Kates J., Kelley D. E., Perry R. P. Polyadenylic acid sequences on 3' termini of vaccinia messenger ribonucleic acid and mammalian nuclear and messenger ribonucleic acid. Biochemistry. 1972 Sep 26;11(20):3829–3834. doi: 10.1021/bi00770a023. [DOI] [PubMed] [Google Scholar]
  14. de Wachter R., Merregaert J., Vandenberghe A., Contreras R., Fiers W. Studies on the bacteriophage MS2. The untranslated 5'-terminal nucleotide sequence preceding the first cistron. Eur J Biochem. 1971 Oct 14;22(3):400–414. doi: 10.1111/j.1432-1033.1971.tb01558.x. [DOI] [PubMed] [Google Scholar]

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