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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1973 Apr;70(4):1037–1040. doi: 10.1073/pnas.70.4.1037

Purification of Mouse Interferon by Affinity Chromatography on a Solid-Phase Immunoadsorbent

Jean D Sipe *, Jaqueline De Maeyer-Guignard , Bernard Fauconnier , Edward De Maeyer
PMCID: PMC433420  PMID: 4352220

Abstract

A solid-phase immunoadsorbent capable of binding mouse interferon has been prepared. Starting from crude tissue-culture material, interferon could be purified 1990 times in a single step of affinity chromatography. Overall recovery ranged from 55 to 103% with tissue culture and mouse-brain interferon; however, only 5% was obtained with Sendai virus-induced interferon from mouse serum.

Keywords: Newcastle disease virus

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cuatrecasas P. Affinity chromatography. Annu Rev Biochem. 1971;40:259–278. doi: 10.1146/annurev.bi.40.070171.001355. [DOI] [PubMed] [Google Scholar]
  2. Cuatrecasas P. Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads. J Biol Chem. 1970 Jun;245(12):3059–3065. [PubMed] [Google Scholar]
  3. Duc-Goiran P., Galliot B., Chany C. Studies on virus-induced interferons produced by the human amniotic membrane and white blood cells. Arch Gesamte Virusforsch. 1971;34(3):232–243. doi: 10.1007/BF01242997. [DOI] [PubMed] [Google Scholar]
  4. Gresser I., Bourali C. Antitumor effects of interferon preparations in mice. J Natl Cancer Inst. 1970 Aug;45(2):365–376. [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Paucker K., Stancek D. Characterization of interferon-associated proteins. J Gen Virol. 1972 May;15(2):129–138. doi: 10.1099/0022-1317-15-2-129. [DOI] [PubMed] [Google Scholar]
  7. Porath J., Axen R., Ernback S. Chemical coupling of proteins to agarose. Nature. 1967 Sep 30;215(5109):1491–1492. doi: 10.1038/2151491a0. [DOI] [PubMed] [Google Scholar]
  8. de Maeyer-Guignard J., de Maeyer E. Effect of antilymphocytic serum on circulating interferon in mice as a function of the inducer. Nat New Biol. 1971 Feb 17;229(7):212–214. doi: 10.1038/newbio229212a0. [DOI] [PubMed] [Google Scholar]

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