Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1973 Apr;70(4):1204–1208. doi: 10.1073/pnas.70.4.1204

Inhibition of Murine Leukemia Virus Replication by Poly(vinyluracil) and Poly(vinyladenine)

Paula M Pitha *, Natalie M Teich †, Douglas R Lowy †, Josef Pitha ‡
PMCID: PMC433458  PMID: 4123932

Abstract

Poly(1-vinyluracil) and poly(9-vinyladenine), as well as the corresponding polynucleotides poly(uridylate) and poly(adenylate), inhibit acute murine leukemia virus infection in mouse-embryo cells, but they do not significantly inhibit the replication of Sindbis and vesicular stomatitis viruses. The polymers were most effective as inhibitors when added during an early stage of virus replication. Effects of vinyl polymers on the RNA-dependent DNA polymerase from the virions of murine leukemia virus were also observed.

Keywords: vesicular stomatitis virus, Sindbis virus, RNA-dependent DNA polymerase, viral chemotherapy

Full text

PDF
1207

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baltimore D., Smoler D. Primer requirement and template specificity of the DNA polymerase of RNA tumor viruses. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1507–1511. doi: 10.1073/pnas.68.7.1507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baltimore D. Viral genetic systems. Trans N Y Acad Sci. 1971 Mar;33(3):327–332. doi: 10.1111/j.2164-0947.1971.tb02600.x. [DOI] [PubMed] [Google Scholar]
  3. Brockman W. W., Carter W. A., Li L. H., Reusser F., Nichol F. R. Streptovaricins inhibit RNA dependent DNA polymerase present in an oncogenic RNA virus. Nature. 1971 Mar 26;230(5291):249–250. doi: 10.1038/230249a0. [DOI] [PubMed] [Google Scholar]
  4. Carrier W. L., Setlow R. B. Paper strip method for assaying gradient fractions containing radioactive macromolecules. Anal Biochem. 1971 Oct;43(2):427–432. doi: 10.1016/0003-2697(71)90272-7. [DOI] [PubMed] [Google Scholar]
  5. Colby C., Chamberlin M. J. The specificity of interferon induction in chick embryo cells by helical RNA. Proc Natl Acad Sci U S A. 1969 May;63(1):160–167. doi: 10.1073/pnas.63.1.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Darnell J. E., Wall R., Tushinski R. J. An adenylic acid-rich sequence in messenger RNA of HeLa cells and its possible relationship to reiterated sites in DNA. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1321–1325. doi: 10.1073/pnas.68.6.1321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Edmonds M., Vaughan M. H., Jr, Nakazato H. Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1336–1340. doi: 10.1073/pnas.68.6.1336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ehrenfeld E., Summers D. F. Adenylate-rich sequences in vesicular stomatitis virus messenger ribonucleic acid. J Virol. 1972 Oct;10(4):683–688. doi: 10.1128/jvi.10.4.683-688.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Haruna I., Spiegelman S. Selective interference with viral RNA formation in vitro by specific inhibition with synthetic polyribonucleotides. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1333–1338. doi: 10.1073/pnas.56.4.1333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Johnston R. E., Bose H. R. Correlation of messenger RNA function with adenylate-rich segments in the genomes of single-stranded RNA viruses. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1514–1516. doi: 10.1073/pnas.69.6.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lai M. M., Duesberg P. H. Adenylic acid-rich sequence in RNAs of Rous sarcoma virus and Rauscher mouse leukaemia virus. Nature. 1972 Feb 18;235(5338):383–386. doi: 10.1038/235383c0. [DOI] [PubMed] [Google Scholar]
  12. Liebeskind D. S., Hsu K. C., Erlanger B. F., Beiser S. M. Selective inhibition of transformed cells in culture by anti-thymidine antibodies. Nat New Biol. 1971 Nov 24;234(47):127–128. doi: 10.1038/newbio234127a0. [DOI] [PubMed] [Google Scholar]
  13. PHILLIPS H. J., TERRYBERRY J. E. Counting actively metabolizing tissue cultured cells. Exp Cell Res. 1957 Oct;13(2):341–347. doi: 10.1016/0014-4827(57)90013-7. [DOI] [PubMed] [Google Scholar]
  14. Pitha J., Pitha P. M., Stuart E. Vinyl analogs of polynucleotides. Biochemistry. 1971 Dec 7;10(25):4595–4602. doi: 10.1021/bi00801a001. [DOI] [PubMed] [Google Scholar]
  15. Pitha J., Pitha P. M., Ts'o P. O. Poly(1-vinyluracil): the preparation and interactions with adenosine derivatives. Biochim Biophys Acta. 1970 Mar 19;204(1):39–48. doi: 10.1016/0005-2787(70)90488-0. [DOI] [PubMed] [Google Scholar]
  16. Pitha P. M., Pitha J. Preparation and properties of poly-9-vinyladenine. Biopolymers. 1970;9(8):965–977. doi: 10.1002/bip.1970.360090809. [DOI] [PubMed] [Google Scholar]
  17. Reynolds F., Grunberger D., Pitha J., Pitha P. M. Inhibition of cell-free protein synthesis by poly(9-vinyladenine), poly(1-vinyluracil), and the corresponding vinyl copolymer. Biochemistry. 1972 Aug 15;11(17):3261–3266. doi: 10.1021/bi00767a021. [DOI] [PubMed] [Google Scholar]
  18. Rowe W. P., Pugh W. E., Hartley J. W. Plaque assay techniques for murine leukemia viruses. Virology. 1970 Dec;42(4):1136–1139. doi: 10.1016/0042-6822(70)90362-4. [DOI] [PubMed] [Google Scholar]
  19. Temin H. M. Mechanism of cell transformation by RNA tumor viruses. Annu Rev Microbiol. 1971;25:609–648. doi: 10.1146/annurev.mi.25.100171.003141. [DOI] [PubMed] [Google Scholar]
  20. Trouet A., Deprez-de Campeneere D., De Duve C. Chemotherapy through lysosomes with a DNA-daunorubicin complex. Nat New Biol. 1972 Sep 27;239(91):110–112. doi: 10.1038/newbio239110a0. [DOI] [PubMed] [Google Scholar]
  21. Tuominen F. W., Kenney F. T. Inhibition of the DNA polymerase of Rauscher leukemia virus by single-stranded polyribonucleotides. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2198–2202. doi: 10.1073/pnas.68.9.2198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Verma I. M., Temple G. F., Fan H., Baltimore D. In vitro synthesis of DNA complementary to rabbit reticulocyte 10S RNA. Nat New Biol. 1972 Feb 9;235(58):163–167. doi: 10.1038/newbio235163a0. [DOI] [PubMed] [Google Scholar]
  23. Zassenhaus P., Kates J. Synthesis of DNA from vaccinia messenger RNA templates. Nat New Biol. 1972 Aug 2;238(83):139–141. doi: 10.1038/newbio238139a0. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES