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. 1973 Apr;70(4):1250–1253. doi: 10.1073/pnas.70.4.1250

Continuous Propagation of Radiation Leukemia Virus on a C57BL Mouse-Embryo Fibroblast Line, with Attenuation of Leukemogenic Activity

Miriam Lieberman 1, Ohtsura Niwa 1, Alain Declève 1, Henry S Kaplan 1
PMCID: PMC433469  PMID: 4352226

Abstract

The radiation leukemia virus (RadLV), a murine leukemia virus derived from thymic lymphomas induced by x-irradiation in strain C57BL/Ka mice, has been successfully propagated in sustained high titer in vitro in a newly established line, BL-5, of C57BL/Ka mouse-embryo fibroblasts. In addition, the production of endogenous virus, presumed to be RadLV, has been induced and sustained through multiple serial passages after treatment of BL-5 cell cultures with 5-bromodeoxyuridine. The chronically RadLV-infected subline, designated BL-5 (RadLV), sheds virus into the supernatant culture fluids that is biologically active in vitro in the XC cell plaque assay, in interference assays for focus-formation by murine sarcoma virus, and in the intracellular induction of group-specific antigens detectable by immunofluorescence, but is apparently devoid of leukemogenic activity after intrathymic inoculation into neonatal or immunosuppressed C57BL/Ka mice. Although BL-5 cells exhibited morphological alterations suggestive of transformation in vitro and gave rise to fibrosarcomatous ascites tumors after intraperitoneal inoculation with C57BL/Ka mice, the chronically infected BL-5(RadLV) cells remained normal in morphology and failed to yield fibrosarcomas in vivo.

Keywords: murine leukemia virus, thymic lymphomas, 5-bromodeoxyuridine

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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