Abstract
The synthesis of the periplasmic galactose-binding protein of E. coli is regulated by events occurring during its cell cycle, and proceeds in synchronized cells for only a short period after cell division is completed. Transport activity mediated by the β-methylgalactoside transport system follows closely the synthesis pattern of the binding protein.
A mutant, E. coli BUG-6, exhibits temperature-sensitive cell division [Reeve et al. (1970) J. Bacteriol. 104, 1052-1064], synthesizing galactose-binding protein at the permissive but not at the nonpermissive temperature. Galactose-binding protein synthesized at the permissive temperature is not degraded after the culture is shifted to the nonpermissive temperature. Polyacrylamide gel electrophoresis of the periplasmic proteins of BUG-6 grown at the permissive and nonpermissive temperatures suggests that several, but not all, periplasmic proteins are subject to the same regulatory control by the cell cycle as the galactose-binding protein.
Keywords: cell division mutant, periplasmic proteins
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ARBER W. Transduction of chromosomal genes and episomes in Escherichia coli. Virology. 1960 May;11:273–288. doi: 10.1016/0042-6822(60)90066-0. [DOI] [PubMed] [Google Scholar]
- Anraku Y. Transport of sugars and amino acids in bacteria. I. Purification and specificity of the galactose- and leucine-binding proteins. J Biol Chem. 1968 Jun 10;243(11):3116–3122. [PubMed] [Google Scholar]
- Boos W., Gordon A. S. Transport properties of the galactose-binding protein of Escherichia coli. Occurrence of two conformational states. J Biol Chem. 1971 Feb 10;246(3):621–628. [PubMed] [Google Scholar]
- Boos W., Lengeler J., Hermann K. O., Unsöld H. J. The regulation of the beta-methylgalactoside transport system and of the galactose binding protein of Escherichia coli K12. Eur J Biochem. 1971 Apr 30;19(4):457–470. doi: 10.1111/j.1432-1033.1971.tb01336.x. [DOI] [PubMed] [Google Scholar]
- Boos W., Sarvas M. O. Close linkage between a galactose binding protein and the beta-methylgalactoside permease in Escherichia coli. Eur J Biochem. 1970 Apr;13(3):526–533. doi: 10.1111/j.1432-1033.1970.tb00956.x. [DOI] [PubMed] [Google Scholar]
- Boos W. Structurally defective galactose-binding protein isolated from a mutant negative in the -methylgalactoside transport system of Escherichia coli. J Biol Chem. 1972 Sep 10;247(17):5414–5424. [PubMed] [Google Scholar]
- Boos W. The galactose binding protein and its relationship to the beta-methylgalactoside permease from Escherichia coli. Eur J Biochem. 1969 Aug;10(1):66–73. doi: 10.1111/j.1432-1033.1969.tb00656.x. [DOI] [PubMed] [Google Scholar]
- Brockman R. W., Heppel L. A. On the localization of alkaline phosphatase and cyclic phosphodiesterase in Escherichia coli. Biochemistry. 1968 Jul;7(7):2554–2562. doi: 10.1021/bi00847a016. [DOI] [PubMed] [Google Scholar]
- Cutler R. G., Evans J. E. Synchronization of bacteria by a stationary-phase method. J Bacteriol. 1966 Feb;91(2):469–476. doi: 10.1128/jb.91.2.469-476.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kubitschek H. E. Constancy of uptake during the cell cycle in Escherichia coli. Biophys J. 1968 Dec;8(12):1401–1412. doi: 10.1016/S0006-3495(68)86562-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kubitschek H. E., Freedman M. L., Silver S. Potassium uptake in synchronous and synchronized cultures of Escherichia coli. Biophys J. 1971 Oct;11(10):787–797. doi: 10.1016/S0006-3495(71)86254-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neu H. C., Heppel L. A. The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. J Biol Chem. 1965 Sep;240(9):3685–3692. [PubMed] [Google Scholar]
- Novick A., Weiner M. ENZYME INDUCTION AS AN ALL-OR-NONE PHENOMENON. Proc Natl Acad Sci U S A. 1957 Jul 15;43(7):553–566. doi: 10.1073/pnas.43.7.553. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oki M. Correlation between metabolism of phosphatidylglycerol and membrane synthesis in Escherichia coli. J Mol Biol. 1972 Jul 21;68(2):249–264. doi: 10.1016/0022-2836(72)90212-4. [DOI] [PubMed] [Google Scholar]
- Reeve J. N., Clark D. J. Cell division of Escherichia coli BUG-6: effect of varying the length of growth at the nonpermissive temperature. J Bacteriol. 1972 Apr;110(1):117–121. doi: 10.1128/jb.110.1.117-121.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reeve J. N., Clark D. J. Cell division of Escherichia coli BUG-6: effect of varying the temperature used as the nonpermissive growth condition. J Bacteriol. 1972 Apr;110(1):122–125. doi: 10.1128/jb.110.1.122-125.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reeve J. N., Groves D. J., Clark D. J. Regulation of Cell Division in Escherichia coli: Characterization of Temperature-Sensitive Division Mutants. J Bacteriol. 1970 Dec;104(3):1052–1064. doi: 10.1128/jb.104.3.1052-1064.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ricciuti C. P. Synchronized division in Escherichia coli: an integral portion of culture growth. J Bacteriol. 1972 Oct;112(1):643–645. doi: 10.1128/jb.112.1.643-645.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robbins A. R., Rotman B. Inhibition of methylgalactoside transport in Escherichia coli upon the cessation of unsaturated fatty acid biosynthesis. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2125–2129. doi: 10.1073/pnas.69.8.2125. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TORRIANI A., ROTHMAN F. Mutants of Escherichia coli constitutive for alkaline phosphatase. J Bacteriol. 1961 May;81:835–836. doi: 10.1128/jb.81.5.835-836.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Winkler H. H., Wilson T. H. The role of energy coupling in the transport of beta-galactosides by Escherichia coli. J Biol Chem. 1966 May 25;241(10):2200–2211. [PubMed] [Google Scholar]
- Wu H. C., Boos W., Kalckar H. M. Role of the galactose transport system in the retention of intracellular galactose in Escherichia coli. J Mol Biol. 1969 Apr 14;41(1):109–120. doi: 10.1016/0022-2836(69)90129-6. [DOI] [PubMed] [Google Scholar]