Abstract
The genes specifying enzymes responsible for the degradation of camphor and octane occur on transmissible plasmids in Pseudomonas putida strain PpG1 and P. oleovorans. Since the presence of the plasmids is vital for the oxidative metabolism of camphor or octane (by the cells) in the absence of other carbon sources, such naturally occurring, energy-generating plasmids have been designated as degradative plasmids. The two degradative plasmids, CAM and OCT, are incompatible with each other and cannot coexist in the same cell. By the use of UV irradiation and suitable selection techniques, it has been possible to fuse these two plasmids so they become part of the same replicon and coexist. Such a technique might be useful in introducing several degradative pathways in the same cell.
Keywords: camphor, energy-yielding pathways, octane
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Selected References
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- Chakrabarty A. M., Chou G., Gunsalus I. C. Genetic regulation of octane dissimilation plasmid in Pseudomonas. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1137–1140. doi: 10.1073/pnas.70.4.1137. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chakrabarty A. M. Genetic basis of the biodegradation of salicylate in Pseudomonas. J Bacteriol. 1972 Nov;112(2):815–823. doi: 10.1128/jb.112.2.815-823.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Novick R. P. Extrachromosomal inheritance in bacteria. Bacteriol Rev. 1969 Jun;33(2):210–263. doi: 10.1128/br.33.2.210-263.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scaife J. Episomes. Annu Rev Microbiol. 1967;21:601–638. doi: 10.1146/annurev.mi.21.100167.003125. [DOI] [PubMed] [Google Scholar]
- Signer E. R. Lysogeny: the integration problem. Annu Rev Microbiol. 1968;22:451–488. doi: 10.1146/annurev.mi.22.100168.002315. [DOI] [PubMed] [Google Scholar]
- Watanabe T. Evolutionary relationships of R factors with other episomes and plasmids. Fed Proc. 1967 Jan-Feb;26(1):23–28. [PubMed] [Google Scholar]