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. 1973 Jun;70(6):1757–1760. doi: 10.1073/pnas.70.6.1757

Replication of Bacteriophage ϕX-174 in a Mutant of Escherichia coli Defective in the dnaE gene

Lorance L Greenlee 1
PMCID: PMC433589  PMID: 4578442

Abstract

Strains of E. coli bearing a temperature-sensitive mutation at the dnaE locus were unable to support growth of bacteriophage ϕX-174 at 43° while permitting normal growth at 30°. The dnaE gene product was implicated in all stages of ϕX-174 DNA replication. Differences were observed between a double mutant (pol A-, dnaEts) and a single mutant (pol A+, dnaEts) in the ability to synthesize parental replicating form and its subsequent replication. The implications of these observations are discussed in terms of the relationship between DNA polymerases-I and -III.

Keywords: DNA polymerases, temperature-sensitive mutants, ϕX-174 DNA

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARNETT W. E., JACOBSON K. B. EVIDENCE FOR DEGENERACY AND AMBIGUITY IN INTERSPECIES AMINOACYL-SRNA FORMATION. Proc Natl Acad Sci U S A. 1964 Apr;51:642–647. doi: 10.1073/pnas.51.4.642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Campbell J. L., Soll L., Richardson C. C. Isolation and partial characterization of a mutant of Escherichia coli deficient in DNA polymerase II. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2090–2094. doi: 10.1073/pnas.69.8.2090. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. De Lucia P., Cairns J. Isolation of an E. coli strain with a mutation affecting DNA polymerase. Nature. 1969 Dec 20;224(5225):1164–1166. doi: 10.1038/2241164a0. [DOI] [PubMed] [Google Scholar]
  4. Francke B., Ray D. S. Formation of the parental replicative form DNA of bacteriophage phi-X174 and initial events in its replication. J Mol Biol. 1971 Nov 14;61(3):565–586. doi: 10.1016/0022-2836(71)90065-9. [DOI] [PubMed] [Google Scholar]
  5. GUTHRIE G. D., SINSHEIMER R. L. Observations on the infection of bacterial protoplasts with the deoxyribonucleic acid of bacteriophage phi X174. Biochim Biophys Acta. 1963 Jun 25;72:290–297. [PubMed] [Google Scholar]
  6. Gefter M. L., Hirota Y., Kornberg T., Wechsler J. A., Barnoux C. Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3150–3153. doi: 10.1073/pnas.68.12.3150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Greenlee L. L. Structural alteration of intracellular phiX174 DNA following infection of host cells in which protein synthesis is inhibited. J Mol Biol. 1970 Oct 28;53(2):163–179. doi: 10.1016/0022-2836(70)90293-7. [DOI] [PubMed] [Google Scholar]
  8. Hirota Y., Gefter M., Mindich L. A mutant of Escherichia coli defective in DNA polymerase II activity. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3238–3242. doi: 10.1073/pnas.69.11.3238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hutchison C. A., 3rd, Sinsheimer R. L. The process of infection with bacteriophage phi-X174. X. Mutations in a phi-X Lysis gene. J Mol Biol. 1966 Jul;18(3):429–447. doi: 10.1016/s0022-2836(66)80035-9. [DOI] [PubMed] [Google Scholar]
  10. Kornberg T., Gefter M. L. Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II. Proc Natl Acad Sci U S A. 1971 Apr;68(4):761–764. doi: 10.1073/pnas.68.4.761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  12. Sinsheimer R. L. Bacteriophage phi-X174 and related viruses. Prog Nucleic Acid Res Mol Biol. 1968;8:115–169. [PubMed] [Google Scholar]
  13. Tessman E. S. Mutants of bacteriophage S13 blocked in infectious DNA synthesis. J Mol Biol. 1966 May;17(1):218–236. doi: 10.1016/s0022-2836(66)80104-3. [DOI] [PubMed] [Google Scholar]
  14. Wechsler J. A., Nüsslein V., Otto B., Klein A., Bonhoeffer F., Herrmann R., Gloger L., Schaller H. Isolation and characterization of thermosensitive Escherichia coli mutants defective in deoxyribonucleic acid replication. J Bacteriol. 1973 Mar;113(3):1381–1388. doi: 10.1128/jb.113.3.1381-1388.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wickner R. B., Wright M., Wickner S., Hurwitz J. Conversion of phiX174 and fd single-stranded DNA to replicative forms in extracts of Escherichia coli. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3233–3237. doi: 10.1073/pnas.69.11.3233. [DOI] [PMC free article] [PubMed] [Google Scholar]

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