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. 1974 Sep;71(9):3367–3371. doi: 10.1073/pnas.71.9.3367

Interactions of a Photo-Affinity ATP Analog with Cation-Stimulated Adenosine Triphosphatases of Human Red Cell Membranes

Boyd E Haley 1,*, Joseph F Hoffman 1
PMCID: PMC433773  PMID: 4279407

Abstract

To identify and isolate ATP binding and hydrolyzing sites of human red cell membranes we have synthesized a photo-activated ATP analog, 8-azido adenosine triphosphate (N3ATP). In the absence of ultraviolet light it is a substrate for both the Mg-ATPase and the ouabain-sensitive, Na,K-ATPase. Hydrolysis of N3ATP is prevented by increasing concentrations of ATP. Photolysis of N3ATP with red cell membranes results in covalent incorporation and irreversible inhibition of both ATPase activities. Also, only three protein components of the red cell membranes are labeled. This labeling is completely abolished by appropriate concentrations of ATP.

Keywords: Na,K-ATPase; red cell ghosts; membrane probe

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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