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. 1974 Sep;71(9):3706–3710. doi: 10.1073/pnas.71.9.3706

Major DNA Polymerases Common to Different Xenopus laevis Cell Types

Franco Tatò 1, Domenica Attardi Gandini 1, Glauco P Tocchini-Valentini 1
PMCID: PMC433845  PMID: 4530330

Abstract

DNA polymerases from Xenopus laevis oocytes, unfertilized eggs, and kidney cells grown in culture have been characterized. The same three major DNA polymerase activities are present in all cell types examined. We attempt to relate the characteristics of amphibian enzymes to those of mammalian polymerases.

Keywords: oocytes, unfertilized eggs, kidney cells

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bolden A., Fry M., Muller R., Citarella R., Weissbach A. The presence of a polyriboadenylic acid-dependent DNA polymerase in eukaryotic cells. Arch Biochem Biophys. 1972 Nov;153(1):26–33. doi: 10.1016/0003-9861(72)90416-x. [DOI] [PubMed] [Google Scholar]
  2. Brown D. D., Dawid I. B. Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA. Science. 1968 Apr 19;160(3825):272–280. doi: 10.1126/science.160.3825.272. [DOI] [PubMed] [Google Scholar]
  3. Burgess R. R., Travers A. A., Dunn J. J., Bautz E. K. Factor stimulating transcription by RNA polymerase. Nature. 1969 Jan 4;221(5175):43–46. doi: 10.1038/221043a0. [DOI] [PubMed] [Google Scholar]
  4. Callan H. G. Replication of DNA in eukaryotic chromosomes. Br Med Bull. 1973 Sep;29(3):192–195. doi: 10.1093/oxfordjournals.bmb.a071006. [DOI] [PubMed] [Google Scholar]
  5. Chang L. M., Brown M., Bollum F. J. Induction of DNA polymerase in mouse L cells. J Mol Biol. 1973 Feb 15;74(1):1–8. doi: 10.1016/0022-2836(73)90349-5. [DOI] [PubMed] [Google Scholar]
  6. Dawid I. B. Deoxyribonucleic acid in amphibian eggs. J Mol Biol. 1965 Jul;12(3):581–599. doi: 10.1016/s0022-2836(65)80313-8. [DOI] [PubMed] [Google Scholar]
  7. Fridlender B., Fry M., Bolden A., Weissbach A. A new synthetic RNA-dependent DNA polymerase from human tissue culture cells (HeLa-fibroblast-synthetic oligonucleotides-template-purified enzymes). Proc Natl Acad Sci U S A. 1972 Feb;69(2):452–455. doi: 10.1073/pnas.69.2.452. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fry M., Weissbach A. A new deoxyribonucleic acid dependent deoxyribonucleic acid polymerase from HeLa cell mitochondria. Biochemistry. 1973 Sep 11;12(19):3602–3608. doi: 10.1021/bi00743a005. [DOI] [PubMed] [Google Scholar]
  9. Gurdon J. B., Birnstiel M. L., Speight V. A. The replication of purified DNA introduced into living egg cytoplasm. Biochim Biophys Acta. 1969 Feb 18;174(2):614–628. doi: 10.1016/0005-2787(69)90291-3. [DOI] [PubMed] [Google Scholar]
  10. Gurdon J. B., Speight V. A. The appearance of cytoplasmic DNA polymerase activity during the maturation of amphibian oocytes into eggs. Exp Cell Res. 1969 May;55(2):253–256. doi: 10.1016/0014-4827(69)90488-1. [DOI] [PubMed] [Google Scholar]
  11. Laskey R. A., Gurdon J. B. Induction of polyoma DNA synthesis by injection into frog-egg cytoplasm. Eur J Biochem. 1973 Sep 3;37(3):467–471. doi: 10.1111/j.1432-1033.1973.tb03007.x. [DOI] [PubMed] [Google Scholar]
  12. Lewis B. J., Abrell J. W., Smith R. G., Gallo R. C. Human DNA polymerase 3 (R-DNA polymerase): distinction from DNA polymerase I and reverse transcriptase. Science. 1974 Mar 1;183(4127):867–869. doi: 10.1126/science.183.4127.867. [DOI] [PubMed] [Google Scholar]
  13. Loeb L. A. Purification and properties of deoxyribonucleic acid polymerase from nuclei of sea urchin embryos. J Biol Chem. 1969 Apr 10;244(7):1672–1681. [PubMed] [Google Scholar]
  14. McCaffrey R., Smoler D. F., Baltimore D. Terminal deoxynucleotidyl transferase in a case of childhood acute lymphoblastic leukemia. Proc Natl Acad Sci U S A. 1973 Feb;70(2):521–525. doi: 10.1073/pnas.70.2.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schorderet-Slatkine S. Action of progesterone and related steroids on oocyte maturation in Xenopus laevis. An in vitro study. Cell Differ. 1972 Aug;1(3):179–189. doi: 10.1016/0045-6039(72)90027-9. [DOI] [PubMed] [Google Scholar]
  16. Sedwick W. D., Wang T. S., Korn D. Purification and properties of nuclear and cytoplasmic deoxyribonucleic acid polymerases from human KB cells. J Biol Chem. 1972 Aug 25;247(16):5026–5033. [PubMed] [Google Scholar]
  17. Smith R. G., Gallo R. C. DNA-dependent DNA polymerases I and II from normal human-blood lymphocytes. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2879–2884. doi: 10.1073/pnas.69.10.2879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stavrianopoulos J. G., Karkas J. D., Chargaff E. Nucleic acid polymerases of the developing chicken embryo: a DNA polymerase preferring a hybrid template. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2207–2211. doi: 10.1073/pnas.68.9.2207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tocchini-Valentini G. P., Mahdavi V., Brown R., Crippa M. The synthesis of amplified ribosomal DNA. Cold Spring Harb Symp Quant Biol. 1974;38:551–558. doi: 10.1101/sqb.1974.038.01.059. [DOI] [PubMed] [Google Scholar]
  20. Weissbach A., Bolden A., Muller R., Hanafusa H., Hanafusa T. Deoxyribonucleic acid polymerase activities in normal and leukovirus-infected chicken embryo cells. J Virol. 1972 Sep;10(3):321–327. doi: 10.1128/jvi.10.3.321-327.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Weissbach A., Schlabach A., Fridlender B., Bolden A. DNA polymerases from human cells. Nat New Biol. 1971 Jun 9;231(23):167–170. doi: 10.1038/newbio231167a0. [DOI] [PubMed] [Google Scholar]
  22. YONEDA M., BOLLUM F. J. DEOXYNUCLEOTIDE-POLYMERIZING ENZYMES OF CALF THYMUS GLAND. I. LARGE SCALE PURIFICATION OF TERMINAL AND REPLICATIVE DEOXYNUCLEOTIDYL TRANSFERASES. J Biol Chem. 1965 Aug;240:3385–3391. [PubMed] [Google Scholar]

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