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. 1974 Oct;71(10):3819–3823. doi: 10.1073/pnas.71.10.3819

Stringent Control of Ribosomal Protein Gene Expression in Escherichia coli*

Patrick P Dennis 1,2,3, Masayasu Nomura 1,2,3
PMCID: PMC434275  PMID: 4610562

Abstract

The regulation of the expression of ribosomal protein genes was examined during partial inhibition of tRNA aminoacylation in isogenic rel+ and rel- strains of E. coli B carrying a temperature-sensitive mutation in valyl-tRNA synthetase (EC 6.1.1.9) gene. Measurements of the differential synthesis rate of ribosomal protein, αr (ribosomal protein synthesis rate/total protein synthesis rate), indicate that ribosomal protein synthesis is regulated directly or indirectly by availability of charged tRNA, and that the synthesis of ribosomal protein, like the synthesis of rRNA, is subject to the influence of the rel gene control system.

Keywords: coordinate regulation of rRNA and ribosomal protein, guanosine tetraphosphate

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brunschede H., Bremer H. Synthesis and breakdown of proteins in Escherichia coli during amino-acid starvation. J Mol Biol. 1971 Apr 14;57(1):35–57. doi: 10.1016/0022-2836(71)90118-5. [DOI] [PubMed] [Google Scholar]
  2. Cashel M., Lazzarini R. A., Kalbacher B. An improved method for thin-layer chromatography of nucleotide mixtures containing 32P-labelled orthophosphate. J Chromatogr. 1969 Mar 11;40(1):103–109. doi: 10.1016/s0021-9673(01)96624-5. [DOI] [PubMed] [Google Scholar]
  3. Davies J., Nomura M. The genetics of bacterial ribosomes. Annu Rev Genet. 1972;6:203–234. doi: 10.1146/annurev.ge.06.120172.001223. [DOI] [PubMed] [Google Scholar]
  4. Dennis P. P., Bremer H. Differential rate of ribosomal protein synthesis in Escherichia coli B/r. J Mol Biol. 1974 Apr 15;84(3):407–422. doi: 10.1016/0022-2836(74)90449-5. [DOI] [PubMed] [Google Scholar]
  5. Dennis P. P., Bremer H. Macromolecular composition during steady-state growth of Escherichia coli B-r. J Bacteriol. 1974 Jul;119(1):270–281. doi: 10.1128/jb.119.1.270-281.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dennis P. P. Regulation of ribosomal and transfer ribonucleic acid synthesis in Escherichia coli B-r. J Biol Chem. 1972 May 10;247(9):2842–2845. [PubMed] [Google Scholar]
  7. Edlin G., Broda P. Physiology and genetics of the "ribonucleic acid control" locus in escherichia coli. Bacteriol Rev. 1968 Sep;32(3):206–226. doi: 10.1128/br.32.3.206-226.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fiil N. P., von Meyenburg K., Friesen J. D. Accumulation and turnover of guanosine tetraphosphate in Escherichia coli. J Mol Biol. 1972 Nov 28;71(3):769–783. doi: 10.1016/s0022-2836(72)80037-8. [DOI] [PubMed] [Google Scholar]
  9. Goodman D. Ribosomal protein synthesis during amino acid starvation and chloramphenicol treatment. J Mol Biol. 1970 Aug;51(3):491–499. doi: 10.1016/0022-2836(70)90003-3. [DOI] [PubMed] [Google Scholar]
  10. Kaltschmidt E., Wittmann H. G. Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Anal Biochem. 1970 Aug;36(2):401–412. doi: 10.1016/0003-2697(70)90376-3. [DOI] [PubMed] [Google Scholar]
  11. NAKADA D., ANDERSON I. A., MAGASANIK B. FATE OF THE RIBOSOMAL RNA PRODUCED BY A "RELAXED" MUTANT OF ESCHERICHIA COLI. J Mol Biol. 1964 Aug;9:472–488. doi: 10.1016/s0022-2836(64)80220-5. [DOI] [PubMed] [Google Scholar]
  12. Parker J., Flashner M., Mckeever W. G., Neidhardt F. C. Metabolic regulation of the arginyl and valyl transfer ribonucleic acid synthetases in bacteria. J Biol Chem. 1974 Feb 25;249(4):1044–1053. [PubMed] [Google Scholar]
  13. SPAHR P. F. Amino acid composition of ribosomes from Escherichia coli. J Mol Biol. 1962 May;4:395–406. doi: 10.1016/s0022-2836(62)80020-5. [DOI] [PubMed] [Google Scholar]
  14. STENT G. S., BRENNER S. A genetic locus for the regulation of ribonucleic acid synthesis. Proc Natl Acad Sci U S A. 1961 Dec 15;47:2005–2014. doi: 10.1073/pnas.47.12.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]

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