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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 Oct;71(10):3866–3868. doi: 10.1073/pnas.71.10.3866

Equilibrium Sedimentation of Turnip Yellow Mosaic Virus

J H McGee 1, D W Kupke *, W Godschalk *, J W Beams 1
PMCID: PMC434285  PMID: 4530267

Abstract

Sedimentation equilibrium was achieved with turnip yellow mosaic virus at low speeds (600 rpm) in a magnetic ultracentrifuge. The experiments were carried out in the newly installed constant-speed rotor, equipped with automatic control and an electromagnetic drive. A particle mass of 5.55 × 106 daltons was calculated for the virus at vanishing concentrations, in essential agreement with the earlier results using the more tedious procedure with a freely coasting rotor. In order to interpret the observed departure from ideal behavior (nonassociating conditions), preferential interaction experiments were carried out by magnetic densimetry. These showed that a strong Donnan effect exists at pH 7, which contributed > 103 times more to the nonideality than did the excluded volume effect. A net negative charge of about 3.5 × 103 per particle was estimated at this pH.

Keywords: magnetic ultracentrifuge, preferential interaction, Donnan effect, molecular weight

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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