Abstract
Objective:
To evaluate the effects of oral delayed-release dimethyl fumarate (DMF; also known as gastro-resistant DMF) on MRI lesion activity and load, atrophy, and magnetization transfer ratio (MTR) measures from the Comparator and an Oral Fumarate in Relapsing-Remitting Multiple Sclerosis (CONFIRM) study.
Methods:
CONFIRM was a 2-year, placebo-controlled study of the efficacy and safety of DMF 240 mg twice (BID) or 3 times daily (TID) in 1,417 patients with relapsing-remitting multiple sclerosis (RRMS); subcutaneous glatiramer acetate 20 mg once daily was included as an active reference comparator. The number and volume of T2-hyperintense, T1-hypointense, and gadolinium-enhancing (Gd+) lesions, as well as whole brain volume and MTR, were assessed in 681 patients (MRI cohort).
Results:
DMF BID and TID produced significant and consistent reductions vs placebo in the number of new or enlarging T2-hyperintense lesions and new nonenhancing T1-hypointense lesions after 1 and 2 years of treatment and in the number of Gd+ lesions at week 24, year 1, and year 2. Lesion volumes were also significantly reduced. Reductions in brain atrophy and MTR changes with DMF relative to placebo did not reach statistical significance.
Conclusions:
The robust effects on MRI active lesion counts and total lesion volume in patients with RRMS demonstrate the ability of DMF to exert beneficial effects on inflammatory lesion activity in multiple sclerosis, and support DMF therapy as a valuable new treatment option in RRMS.
Classification of evidence:
This study provides Class I evidence of reduction in brain lesion number and volume, as assessed by MRI, over 2 years of delayed-release DMF treatment.
Multiple sclerosis (MS) is a chronic disease of the CNS, characterized by inflammatory demyelination and axonal degeneration, producing focal lesions of white and gray matter. Much of the damage in MS is thought to be caused by inflammation and associated oxidative stress; treatments that improve cellular resistance to inflammatory insults may broaden available options in MS therapy.
Oral delayed-release dimethyl fumarate (DMF; also known as gastro-resistant DMF) has demonstrated anti-inflammatory and cytoprotective effects mediated via both the nuclear factor (erythroid-derived 2)–like 2 (Nrf2) pathway and Nrf2-independent pathways in preclinical studies.1–4 Two randomized, double-blind, phase 3 studies, Determination of the Efficacy and Safety of Oral Fumarate in Relapsing-Remitting Multiple Sclerosis (DEFINE) and Comparator and an Oral Fumarate in Relapsing-Remitting Multiple Sclerosis (CONFIRM), were conducted among patients with relapsing-remitting MS (RRMS).5,6 In both studies, DMF treatment produced significant clinical and neuroradiologic benefits over 2 years, including reductions in relapses, new lesion counts, and lesion volumes assessed by MRI, with an acceptable safety profile.
DMF is approved in the United States for the treatment of relapsing forms of MS.7 Here, we report the effects of DMF on MRI brain lesions observed in CONFIRM. Tertiary analyses were also conducted to assess changes in brain volume (atrophy) and in magnetization transfer ratio (MTR), a quantitative MRI technique used to detect changes in intrinsic tissue structure, including myelin content, in the CNS.8–10
METHODS
Standard protocol approvals, registrations, and patient consents.
The CONFIRM study (ClinicalTrials.gov identifier NCT00451451) was approved by central and local ethics committees and performed in accordance with The International Conference on Harmonisation Guidelines on Good Clinical Practice11 and the ethical principles outlined in the Declaration of Helsinki.12 Written informed consent was obtained from all patients before evaluations were performed to determine eligibility.
Study design.
CONFIRM was a 2-year, phase 3, randomized, international, multicenter, parallel-group, placebo-controlled study, with active reference comparator, that evaluated the safety and efficacy of DMF in patients with RRMS, as reported previously.6 Patients from 200 sites in 28 countries were randomized 1:1:1:1 to receive PO placebo, PO DMF 240 mg twice daily (BID), PO DMF 240 mg 3 times daily (TID), or glatiramer acetate (GA) 20 mg once daily by subcutaneous injection for 96 weeks. DMF and placebo treatments were double-blind, while GA treatment was rater-blind.
MRI secondary and tertiary outcomes of the study were assessed in a subset of patients at centers with appropriate imaging facilities. These included the number of new or enlarging T2-hyperintense, new nonenhancing T1-hypointense, and gadolinium-enhancing (Gd+) lesions; volume of T2-hyperintense, nonenhancing T1-hypointense, and Gd+ lesions; percentage brain volume change (PBVC); and percentage change in whole brain MTR.
Patients.
As reported previously,6 patients aged 18–55 years with a confirmed diagnosis of RRMS according to McDonald criteria 200513 and an Expanded Disability Status Scale (EDSS) score of 0–5.0 were enrolled in the MRI portion of the trial at screening, prior to randomization (figure 1). Additional eligibility criteria are included in e-Methods on the Neurology® Web site at Neurology.org.
MRI assessments.
MRI scans were obtained at baseline and at weeks 24, 48, and 96 in patients from a subset of sites with full MRI capabilities. Approximately 95% of patients at these sites chose to participate. Original MRI scans from each site were evaluated in a blinded manner by qualified personnel at a central reading facility (University College London Institute of Neurology, UK).
Lesion volumes (T2-weighted and nonenhancing T1-weighted) were assessed at baseline and weeks 48 and 96, and numbers of new or enlarging T2-weighted lesions and new nonenhancing T1-weighted lesions were assessed at weeks 48 and 96. Gd+ lesion count and volume were assessed at baseline and weeks 24, 48, and 96. Lesion assessment methodology is provided in e-Methods.
Normalized brain volume was assessed at baseline and week 24 using the structural image evaluation using normalization of atrophy (SIENA) cross-sectional (SIENAX) method, and PBVC was assessed at each post-baseline MRI visit relative to earlier measurements by the SIENA method.14 A more stringent post hoc quality assessment and analysis of PBVC is also included (methodology described in e-Methods). MTR analysis was conducted using a purposefully developed pipeline (methodology described in e-Methods). Median whole brain MTR at baseline, week 48, and week 96 was reported.
Statistical analyses.
The intent-to-treat (ITT) population comprised all randomized patients who received at least one dose of study treatment. The MRI cohort comprised ITT patients who consented to participate in the MRI portion of the study and had any MRI data.
A negative binomial regression model was used to analyze the total number of new or enlarging T2-hyperintense lesions and the number of new T1-hypointense lesions at 2 years (secondary endpoints) and 1 year (tertiary endpoints). The model was adjusted for region (defined by type of health care system, access to health care, and geography) and baseline lesion volume (T1-hypointense or T2-hyperintense). The number of Gd+ lesions at 1 and 2 years (tertiary endpoints) were analyzed using an ordinal logistic regression model, adjusted for region and baseline Gd+ lesion number.
Analysis of covariance (ANCOVA) on ranked data was used to assess the effects of treatment on volume of T1-hypointense, T2-hyperintense, and Gd+ lesions at 1 and 2 years (tertiary endpoints). The model included treatment group and adjustments for region and baseline lesion volume (T1-hypointense, T2-hyperintense, or Gd+).
ANCOVA of ranked data was used to assess the effects of treatment on PBVC at 2 years, relative to baseline and relative to week 24. The model included treatment group and adjustments for region and brain volume at reference visit (baseline or week 24).
ANCOVA was also used to assess the effects of treatment on the percentage change from baseline of whole brain MTR at 1 and 2 years. The model included treatment group and adjustments for region and baseline MTR value.
Linear relationships between MRI endpoints and clinical endpoints were calculated using Spearman rank correlation coefficients.
Classification of evidence.
This study provides Class I evidence of reduction in brain lesion number and volume, as assessed by MRI, over 2 years of treatment with DMF 240 mg BID or TID, compared with placebo, in patients with RRMS. DMF BID and TID reduced the mean number of new or enlarging T2-hyperintense lesions by 71% and 73%, respectively, the mean number of new nonenhancing T1-hypointense lesions by 57% and 65%, and Gd+ lesions by 74% and 65% (reductions in the odds of having more lesions) over 2 years vs placebo (all p < 0.001). Lesion volumes were also significantly reduced.
RESULTS
Patients.
Of the 1,430 patients randomized, 1,417 were included in the ITT population, and 681 patients in the MRI cohort (figure 1). Baseline demographic and disease characteristics of the MRI cohort were comparable among treatment groups (table 1) and with the non-MRI cohort (tables e-1 and e-2).
Table 1.
Lesion number.
Compared with placebo, DMF BID and TID reduced the mean number of new or enlarging T2-hyperintense lesions that developed over 2 years by 71% and 73%, respectively (both p < 0.0001) (figure 2A and table e-3). This robust effect was evident at 1 year and was maintained at 2 years. DMF reduced the number of new or enlarging T2-hyperintense lesions by 67% (BID) and 70% (TID) during the first year, and by 76% (both groups) during the second year (all p < 0.0001). In the GA group, the relative reduction in mean T2-hyperintense lesion number vs placebo was 54% over 2 years: 52% in the first year and 57% in the second year (all p < 0.0001).
Compared with placebo, DMF reduced the mean number of new nonenhancing T1-hypointense lesions that developed over 2 years by 57% (BID) and 65% (TID) (p < 0.0001 for both comparisons), with respective relative reductions of 41% (p = 0.0030) and 60% (p < 0.0001) in the first year, and 70% and 71% (both p < 0.0001) in the second year (figure 2B and table e-4). Relative to placebo, GA reduced the mean new nonenhancing T1-hypointense lesion number by 41% (p = 0.0021) over the 2 years: 30% in the first year and 55% in the second year (p = 0.0402 and 0.0002, respectively).
DMF also reduced Gd+ lesions at 6 months, with relative reductions over placebo of 81% (BID) and 69% (TID) (reductions in the odds of having more lesions; p < 0.0001 for both comparisons); the relative reduction with GA at this time point was 42% (p = 0.0201) (figure 2C and table e-5). Relative odds reductions over placebo in Gd+ lesions were 87% (BID), 77% (TID), and 63% (GA) (all p < 0.0001) at 1 year and 74% (BID, p < 0.0001), 65% (TID, p = 0.0001), and 61% (GA, p = 0.0003) at 2 years.
The robustness of these findings was confirmed by sensitivity analyses based on all observed data, and on observed data prior to starting alternative MS medication (figure e-1). These sensitivity analyses indicated that neither the data imputation method used in the primary analysis nor the exclusion of data after patients switched to alternative MS medication affected the results.
Although this study was not designed to assess superiority or noninferiority of DMF over GA, the reductions in lesion numbers over 2 years relative to placebo were numerically greater in both DMF dose groups than in the GA group. Direct comparisons (post hoc) of the relative effect of BID and TID vs GA produced rate ratios (95% confidence intervals [CI]) of 0.64 (0.46, 0.88) and 0.59 (0.43, 0.82) for new/enlarging T2-hyperintense lesions (nominal p values for the comparison: p = 0.0068 and p = 0.0017), and 0.74 (0.52, 1.04) and 0.59 (0.41, 0.84) for new nonenhancing T1-hypointense lesions (nominal p = 0.0846 and p = 0.0033), respectively. Odds ratios (95% CI) for Gd+ lesions were 0.71 (0.40, 1.25) for BID and 0.87 (0.50, 1.52) TID vs GA (not significant).
Lesion volume.
A reduction in T2-hyperintense lesion volume was seen within the first year of DMF treatment, and was maintained throughout the study. Lesion volume increased in the placebo group, with mean ± SD change from baseline to 2 years of 744.7 ± 3,662.4 mm3, representing a median 15% increase from baseline, vs a reduction in T2-hyperintense lesion volume by 1,035.8 ± 2,657.2, 800.5 ± 3,933.3, and 946.0 ± 3,766.6 mm3 (median percentage change −7%, −2%, and −6%) in the BID, TID, and GA groups, respectively (p < 0.0001 for all comparisons vs placebo) (figure 2D and table e-3).
Increases in nonenhancing T1-hypointense lesion volume at 2 years were attenuated with DMF treatment relative to placebo, with mean ± SD changes from baseline of 198.1 ± 1,380.0 (BID) and 190.7 ± 1,204.2 (TID), vs 342.4 ± 878.5 mm3 with placebo (median percentage change 11% and 9% vs 20%; p = 0.0005 and p = 0.0015 vs placebo), respectively (figure 2E and table e-4). Treatment with GA also attenuated the increase in T1-hypointense lesion volume at 2 years relative to placebo, with mean ± SD change from baseline of 214.7 ± 1,389.0 mm3 (median percentage change 9%, p = 0.0013 vs placebo).
Compared with placebo, DMF reduced Gd+ lesion volumes, with mean ± SD values at 2 years of 35.9 ± 128.5 (BID) and 42.6 ± 149.9 mm3 (TID), compared with 141.8 ± 339.1 mm3 for placebo (post hoc analysis; both p < 0.0001 vs placebo) (figure 2F and table e-5). This treatment effect was apparent within 6 months. The mean ± SD reduction from baseline to 2 years in Gd+ lesion volume was 251.8 ± 595.6 (BID) and 181.7 ± 738.5 mm3 (TID), compared with 7.2 ± 448.6 mm3 (placebo), and the median percentage change from baseline was −100% (BID) and −100% (TID) (both p < 0.0001 vs placebo, +14%) (table e-5). In patients treated with GA, Gd+ lesion volume was 45.6 ± 135.8 mm3 at 2 years (post hoc analysis; p < 0.0001 vs placebo) and was reduced from baseline to 2 years by 202.5 ± 573.5 mm3 (median percentage change from baseline −100%, p < 0.0001 vs placebo).
Brain atrophy and whole brain MTR.
Median PBVC from baseline to 2 years was less than 1% in the placebo group as well as the active treatment groups (figure 3), indicating a relatively low average rate of atrophy during the study. Data from baseline to week 24 showed no evidence of pseudoatrophy in active treatment arms (table e-6). At 2 years, PBVC favored DMF, but not GA, relative to placebo, although none of the changes reached statistical significance: the median decrease in PBVC was 30% (BID; p = 0.0645) and 21% (TID; p = 0.2636) relative to placebo, vs a median relative increase of 2% for GA vs placebo (p = 0.8802). Between years 1 and 2, there was a 32% reduction in atrophy with DMF relative to placebo (p = 0.0359 [BID] and p = 0.0755 [TID]), and 28.8% reduction in the GA group (p = 0.0805), based on median PBVC values. Similar results were obtained in the more stringent post hoc quality assurance reanalysis of PBVC (table e-7). At 2 years, mean (median) percentage changes in MTR from baseline were −0.167 (0.000), −0.008 (0.000), and +0.010 (0.000) for DMF BID, TID, and GA groups, respectively, compared with −0.419 (−0.323) for placebo (table e-8); none of the relative changes in MTR reached statistical significance compared with placebo.
Subgroup analysis.
Compared with placebo, DMF (BID or TID) was associated with robust reductions in numbers of new/enlarging T2-hyperintense lesions and Gd+ lesions across a range of prespecified patient subpopulations stratified by baseline characteristics (sex, age <40 vs ≥40 years, ≤1 vs ≥2 relapses in the year prior to study, McDonald criteria, prior MS treatment status, EDSS score, T2 lesion volume, and Gd+ lesion status) (figure e-2).
Clinical–MRI correlations.
In the overall cohort after 2 years, there were significant correlations of change in T2 and T1 lesion load and in new lesion counts with relapse rate, and no significant correlation of PBVC (tertiary endpoint) with change in EDSS (table e-9).
DISCUSSION
MRI is an important tool for monitoring disease activity and is widely used in clinical trials of MS therapies. In this study, we have shown that DMF significantly reduced disease activity as measured by MRI active lesion number (an indicator of recent inflammation that correlates with relapse rates)15,16 and disease progression, as measured by total lesion volume (an indicator of overall disease burden),17 compared with placebo. These effects were evident at the first MRI assessment for T2-hyperintense lesions and Gd+ lesions (1 year and 6 months, respectively) and persisted over the 2-year evaluation period. Furthermore, these findings were accompanied by reductions in new nonenhancing T1-hypointense lesions relative to placebo, which may suggest an effect of DMF in reducing tissue damage since persistent T1-hypointense lesions are known to correlate with axonal loss.18
The MRI lesion outcomes observed in CONFIRM are consistent with the robust benefits reported in the phase 3 DEFINE study5 and the phase 2 clinical study with DMF.19–21 Although interpretation of comparisons between studies is limited by many considerations, including differences in study design, populations, endpoints, and standards of care at the time of study conduct, these MRI lesion outcomes for patients treated with DMF are generally comparable with those reported in other phase 3 studies of investigational and marketed oral MS therapies.5,22–25 The MRI findings observed with DMF also compare favorably with the MRI lesion outcomes seen in the GA arm of this trial, reinforcing the findings of indirect comparison of clinical outcomes of this study that have been reported elsewhere.6
The MRI outcomes in CONFIRM and other clinical studies are consistent with preclinical data,2,4,26 suggesting that anti-inflammatory properties may contribute to the efficacy of DMF in RRMS. This is illustrated by the significant reductions in new or enlarging T2-hyperintense, new T1-hypointense, and Gd+ lesion counts, and total lesion volume. The MRI benefits of DMF are observed across patient subgroups differing in baseline disease activity and demographic characteristics (despite limited patient numbers), further supporting the potential value of this treatment across a broad spectrum of patients with RRMS.
MTR has not been explored previously in a large phase 3 study and, therefore, investigation of MTR in the present study and companion phase 3 study, DEFINE, represents a novel approach to obtain exploratory information and guide future study design. Brain atrophy data were also collected for tertiary analysis. Statistically significant treatment effects of DMF on MTR outcomes (both doses) and brain atrophy (BID only) were observed in DEFINE.27,28 However, MTR outcomes and brain atrophy results with DMF did not reach statistical significance in CONFIRM. The rate of atrophy in the placebo arm during the CONFIRM study was lower in comparison with the placebo arms of other phase 3 studies of oral MS therapies.22,24 In addition, there was no increase in brain volume loss with DMF during the first 24 weeks, indicating no significant pseudoatrophy effect due to a decrease in inflammatory tissue and edema.
Neither of the phase 3 studies was prospectively powered to detect a treatment effect on brain atrophy and MTR. Assessments of these parameters were performed in the MRI cohort that was about half the size of the whole trial population. The relatively small changes in MTR and atrophy measures in all study arms, including placebo, combined with the smaller sample size, may have reduced the sensitivity to detect a significant therapeutic effect over 2 years. A delayed onset of effect may also be relevant, as suggested by more evident reductions in PBVC in the DMF arms during the second year.
Previous trials of GA in clinically isolated syndromes29 and RRMS30 revealed 28% less brain atrophy and no difference in brain volume change after a mean follow-up of 3.8 years and 18 months, respectively. Although we did not observe less PBVC over 2 years in the GA-treated arm vs placebo, there was a trend for less atrophy in the second year.
Overall, the CONFIRM imaging data are consistent with DMF having robust anti-inflammatory effects. The positive imaging findings, when considered alongside clinical relapse and disability data, as well as the acceptable tolerability and safety profile of this agent,5,6 support the use of DMF as a valuable new treatment option in RRMS.
Supplementary Material
GLOSSARY
- ANCOVA
analysis of covariance
- BID
twice daily
- CI
confidence interval
- CONFIRM
Comparator and an Oral Fumarate in Relapsing-Remitting Multiple Sclerosis
- DEFINE
Determination of the Efficacy and Safety of Oral Fumarate in Relapsing-Remitting Multiple Sclerosis
- DMF
delayed-release dimethyl fumarate (also known as gastro-resistant dimethyl fumarate)
- EDSS
Expanded Disability Status Scale
- GA
glatiramer acetate
- Gd+
gadolinium-enhancing
- ITT
intent-to-treat
- MS
multiple sclerosis
- MTR
magnetization transfer ratio
- Nrf2
nuclear factor (erythroid-derived 2)–like 2
- PBVC
percentage brain volume change
- RRMS
relapsing-remitting multiple sclerosis
- SIENA
structural image evaluation using normalization of atrophy
- SIENAX
structural image evaluation using normalization of atrophy cross-sectional
- TID
3 times daily
Footnotes
Supplemental data at Neurology.org
Contributor Information
Collaborators: Robert J. Fox, J. Theodore Phillips, Michael Hutchinson, Eva Havrdova, Mariko Kita, Kate Wilson, Minhua Yang, Katherine T. Dawson, Jack Antel, James Ware, Chris Polman, Peter R. Kowey, Raymond Chung, George Bakris, John Richert, Burt Seibert, David Brandes, David Brassat, Bruce Cohen, Ricarda Diem, Myla Goldman, Robert Herndon, Aaron Miller, Hayrettin Tumani, Teresa Alfaro-Vidal, Carolina Crespo, Jo Foster, Kelvin Hunter, Almudena Garcia-Gomez, David MacManus, David Miller, Virginia Santana, Dan Tozer, Claudia Wheeler-Kingshott, Tarek Yousry, Christopher Kneebone, Aliaksandr Fedulau, Sergey Likhachev, Elena Mikhailova, Halina Naumova, Danny Decoo, Luc Vande Gaer, Christian Sindic, Sanja Grgic, Osman Sinanovic, Enra Mehmedika Suljic, Nadezhda Deleva, Dimitar Georgiev, Lyubomir Haralanov, Sonyia Ivanova, Ivan Manchev, Dimitar Minchev, Paraskeva Stamenova, Ivailo Tournev, Elena Vacheva, Zahari Zahariev, Amit Bar-Or, Gregg Blevins, Marcelo Kremenchutzky, Felix Veloso, Norbert Witt, Roberto Vargas Howell, Alexander Parajeles Vindas, Mario Habek, Josip Rudež, Silva Soldo-Butković, Ranka Baraba Vurdelja, David Doležil, Eva Havrdova, Jiří Nova'k, Daniel Vaclavik, Katrin Antsov, Katrin Gross-Paju, Sulev Haldre, Alla Palu, Toomas Toomsoo, Abdullatif Al Khedr, William Camu, Marc Debouverie, Gilles Defer, Jérôme De Seze, Pierre Labauge, Thibault Moreau, Jean Pelletier, Lucien Rumbach, Vera Daskalovska, Klemens Angnstwurm, Heike Benes, Achim Berthele, Hans-Jürgen Boldt, Angelika Christopher, Tobias Derfuß, Ilonka Eisensehr, Peter Emrich, Wolfgang Feneberg, Frank Hoffmann, Reinhard Hohlfeld, Reinhard Hüntemann, Boris-Alexander Kallmann, Bernd Kieseier, Harald Landefeld, Wilfried Lüer, Sabine Masri, Gereon Nelles, Patrick Oschmann, Christine Paschen, Gerd Reifschneider, Michael Sailer, Sebastian Schimrigk, Mechthild Spiegel-Meixensberger, Brigitte Storch-Hagenlocher, Björn Tackenberg, Klaus Tiel-Wilck, Clementine Karageorgiou, Panagiotis Papathanasopoulos, Thomas Thomaides, Nicholas Vlaikidis, Deepak Arjundas, Madhuri Behari, Amitabha Ghosh, Pahari Ghosh, Nasli Rustom Ichaporia, Dheeraj Khurana, Rahul Vitthal Kulkarni, Suresh Kumar, Man Mohan Mehndiratta, Neeta Abhay Mehta, Usha Kant Misra, Joy Dev Mukherji, Shankara Nellikunja, Abdul Salem, Prahlad Kumar Sethi, Shalin Dipinkumar Shah, Gagandeep Singh, Maneesh Kumar Singh, Yash Pal Singh, Rangasetty Srinivasa, Krishnan Vijayan, Michael Hutchinson, Bernard Sweeney, Ronit Gilad, Radi Shahien, Anita Paegle, Cesar Delgado, Juan Escamilla, Bruno Estañol, Minerva Lopez, Miguel Angel Macias, Guillermo Punzo, Sandra Quiñones, Mariela Renteria, Jose Santos, Mihail Gavriliuc, Stanislav Groppa, Olesea Odainic, Paul Timmings, Anna Czlonkowska, Malgorzata Dorobek, Wieslaw Drozdowski, Waldemar Fryze, Hanka Hertmanowska, Anna Kaminska, Magdalena Kapelusiak-Pielok, Magdalena Kleczkowska, Jan Kochanowicz, Jacek Losy, Przemyslaw Nowacki, Walenty Nyka, Krystyna Pierzchala, Ryszard Podemski, Andrzej Potemkowski, Krzysztof Selmaj, Zbigniew Stelmasiak, Andrzej Szczudlik, Andrzej Tutaj, Andrzej Wajgt, Tomasz Zielinski, Rodica Balasa, Valentin Ionescu-Dimancea, Petru Mihancea, Cristian Popescu, Liviu Codrut Protosevici, Svetlana Miletić Drakulić, Congor Nadj, Ranko Raicevic, Slobodan Vojinovic, Edita Kahancová, Egon Kurca, L'ubomir Lisý, Peter Turčáni, Rafael Arroyo, Oscar Fernández, Cristina Guijarro, Guillermo Izquierdo, Fernando Sanchez Lopez, Xavier Montalbán, Celia Oreja-Guevara, Jose Maria Prieto, Nataliya Buchakchyys'ka, Galyna Chmyr, Alla Goloborodko, Tetyana Kobys, Grygory Kushnir, Volodymyr Lebedynets, Nataliya Lytvynenko, Sergii Moskovko, Tetyana Nehrych, Borys Palamar, Igor Pasyura, Tatyana Ryabichenko, Nataliya Voloshina, Michelle Apperson, Angela Applebee, Stephen Asher, Ricardo Ayala, Donald Ayres, S. Ausim Azizi, Matthew Baker, Brendan Bauer, Roberto Bomprezzi, Richard Buckler, Walter Carlini, Angel Chinea, Stanley Cohan, Giles Crowell, Keith Edwards, Geoffery Eubank, Warren Felton, III, Patricia Fodor, John Foley, Corey Ford, Edward Fox, Robert Fox, Mary Forester, Steven Freedman, George Garmany, Jr, Suzanne Gazda, Daniel Giang, Braeme Glaun, Scott Gold, Malcolm Gottesman, Mark Gudesblatt, Joseph Herbert, Allan Herskowitz, William Honeycutt, John Huddlestone, Richard Hull, Samuel Hunter, George Hutton, Dina Jacobs, Mark Janicki, Bhupendra Khatri, Revere Philip Kinkel, Mariko Kita, Stanley Krolczyk, Lauren Krupp, Christopher LaGanke, Michael Levin, Jonathan Licht, Christopher Luzzio, Sharon Lynch, David Mattson, Daniel Mikol, Tamara Miller, Alireza Minagar, Galen Mitchell, Harold Moses, Jr, Donald Negroski, Stephen Newman, Gabriel Pardo, Malti Patel, Allan Perel, Joseph Phillips, Jr, Mary Ann Picone, Kottil Rammohan, T. Hemanth Rao, John Rinker, II, Saud Sadiq, John Schaeffer, William Sheremata, Robert Shin, Richard Shubin, Stuart Silverman, Robert Smith, Lee Stein, Michael Stein, David Steiner, Brian Steingo, Herman Sullivan, William Sunter, Jr, Anand Vaishnav, Alberto Vasquez, James Voci, Jonathan Warach, Bianca Weinstock-Guttman, Mitzi Williams, and Sibyl Wray
AUTHOR CONTRIBUTIONS
D.H.M. assisted in designing the study, supported centralized collection of the MRI data, overviewed analysis and interpretation of the study data, and cowrote the manuscript. R.J.F., J.T.P., E.H., and M.K. designed the study, collected and interpreted the data, and reviewed the manuscript. M.H. served on the medical advisory board, reviewed drafts, and was involved in data collection. C.A.M.W.-K., D.J.T., D.G.M., and T.A.Y. collected, analyzed, and interpreted the data and reviewed the manuscript. M.G. conducted literature searches, wrote an earlier draft of the manuscript, prepared figures, and edited the manuscript under the direction of the other authors. M.Y. was involved in the study design, analyzed the data, and reviewed the manuscript. R.Z. analyzed the data and reviewed the manuscript. V.V. interpreted the data and reviewed the manuscript. K.T.D. designed the study, interpreted the data, and cowrote the manuscript.
STUDY FUNDING
The CONFIRM study was funded by Biogen Idec Inc., Weston, MA. Medical writing and editorial support were provided by CircleScience (Tytherington, UK) and funded by Biogen Idec Inc. The NMR Research Unit at the Queen Square MS Centre is supported by the UK MS Society and UCLH-UCL Biomedical Research Centre.
DISCLOSURE
D. Miller has received honoraria through payments to his employer, UCL Institute of Neurology, for advisory committee and/or consultancy advice in multiple sclerosis studies from Bayer Schering Pharma, Biogen Idec, Novartis, Mitsubishi Pharma Europe, Merck, and GlaxoSmithKline; and research support through payments to his employer, UCL Institute of Neurology, from Apitope, Biogen Idec, GlaxoSmithKline, Novartis, and Schering AG. R. Fox reports receiving consulting fees from Allozyne, Avanir, Biogen Idec, Novartis, Questcor, and Teva; and grant and research support from Novartis. J. Phillips reports receiving consulting fees from Acorda, Biogen Idec, Genzyme, Novartis, and Teva; and research support from Biogen Idec and Roche. M. Hutchinson has received speaker's honoraria from Bayer Schering, Biogen Idec, and Novartis; editorial fees from the Multiple Sclerosis Journal; and research grants from the Health Research Board, Ireland, and Dystonia Ireland. E. Havrdova reports receiving consulting and lecture fees from Bayer, Biogen Idec, Genzyme, Novartis, Serono, and Teva; and research support from the Czech Ministry of Education (MSM 0021620849, PRVOUK-P26/LF1/4). M. Kita has received travel support and research grant funding from Biogen Idec and served on a speaker's bureau for Genzyme. C. Wheeler-Kingshott reports receiving consulting fees from Biogen Idec. D. Tozer has a portion of his salary paid by Biogen Idec and Novartis for work involving analysis of MRI data in MS trials. D. MacManus has received research grants (held by University College London) from Apitope, Biogen Idec, GlaxoSmithKline, Novartis, Richmond Pharma, and Schering AG for analysis of MRI data in MS trials. T. Yousry has received honoraria and travel expenses for advisory committee work from Bayer Schering, Biogen Idec, and Novartis; and research grants (held by University College London) from Biogen Idec, GlaxoSmithKline, Novartis, and Schering AG for analysis of data from MS trials. M. Goodsell was an employee of CircleScience at the time of writing, which has been contracted by Biogen Idec to provide medical writing services. M. Yang reports being an employee of and holding stock in Biogen Idec. R. Zhang reports being an employee of and holding stock in Biogen Idec. V. Viglietta reports being an employee of and holding stock in Biogen Idec. K. Dawson reports being an employee of and holding stock in Biogen Idec. Go to Neurology.org for full disclosures.
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