Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1980 Feb;65(2):392–396. doi: 10.1104/pp.65.2.392

Chlorophyll-Protein Complexes of the Cyanophyte, Nostoc sp. 1

Mary Rusckowski 1, Barbara A Zilinskas 1
PMCID: PMC440335  PMID: 16661198

Abstract

Four chlorophyll-protein complexes have been resolved from the cyanophyte, Nostoc sp., by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis at 4 C. Complexes solubilized by SDS from Spinacia oleracea were run for comparison. As has been well documented, the P700-chlorophyll a-protein complex from the higher plant and blue-green algal samples are similar, and the light-harvesting pigment protein complex is present only in the former. Most noteworthy are two closely migrating chlorophyll proteins in Nostoc sp. which have approximately the same mobility as a single chlorophyll-protein band resolvable from spinach. The absorption maximum of the complex from spinach is at 667 nanometers, and those of the two complexes from Nostoc sp. are at 667 and 669 nanometers; the fluorescence emission maximum at −196 C is at 685 nanometers, and the 735 nanometer fluorescence peak, characteristic of the P700-chlorophyll a-protein complex, is absent. The apoproteins of these new complexes from Nostoc sp. and spinach are in the kilodalton range. It appears that at least one of these two chlorophyll-protein complexes from Nostoc sp. compares with those recently described by others from higher plants and green algae as likely photosystem II complexes, perhaps containing P680, although no photochemical data are yet available.

Full text

PDF
393

Images in this article

393Image
on p.393

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Apel K., Bogorad L., Woodcock C. L. Chloroplast membranes of the green alga Acetabularia mediterranea. I. Isolation of the photosystem II. Biochim Biophys Acta. 1975 Jun 17;387(3):568–579. doi: 10.1016/0005-2728(75)90094-8. [DOI] [PubMed] [Google Scholar]
  2. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chua N. H., Bennoun P. Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutant strains deficient in photosystem II reaction center. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2175–2179. doi: 10.1073/pnas.72.6.2175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chua N. H., Matlin K., Bennoun P. A chlorophyll-protein complex lacking in photosystem I mutants of Chlamydomonas reinhardtii. J Cell Biol. 1975 Nov;67(2PT1):361–377. doi: 10.1083/jcb.67.2.361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Delepelaire P., Chua N. H. Lithium dodecyl sulfate/polyacrylamide gel electrophoresis of thylakoid membranes at 4 degrees C: Characterizations of two additional chlorophyll a-protein complexes. Proc Natl Acad Sci U S A. 1979 Jan;76(1):111–115. doi: 10.1073/pnas.76.1.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dietrich W. E., Jr, Thornber J. P. The P700-chlorophyll -protein of a blue-green alga. Biochim Biophys Acta. 1971 Sep 7;245(2):482–493. doi: 10.1016/0005-2728(71)90164-2. [DOI] [PubMed] [Google Scholar]
  7. Dunkley P. R., Anderson J. M. The light-harvesting chlorophyll a/b-protein complex from barley thylakoid membranes. Polypeptide composition and characterization of an oligomer. Biochim Biophys Acta. 1979 Jan 11;545(1):174–187. [PubMed] [Google Scholar]
  8. Gray B. H., Lipschultz C. A., Gantt E. Phycobilisomes from a blue-green alga Nostoc species. J Bacteriol. 1973 Oct;116(1):471–478. doi: 10.1128/jb.116.1.471-478.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Henriques F., Park R. B. Characterization of three new chlorophyll-protein complexes. Biochem Biophys Res Commun. 1978 Apr 28;81(4):1113–1118. doi: 10.1016/0006-291x(78)91251-2. [DOI] [PubMed] [Google Scholar]
  10. Henriques F., Park R. B. Spectral characterization of five chlorophyll-protein complexes. Plant Physiol. 1978 Dec;62(6):856–860. doi: 10.1104/pp.62.6.856. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Henriques F., Park R. Polypeptide composition of chlorophyll-protein complexes from romaine lettuce. Plant Physiol. 1977 Jul;60(1):64–68. doi: 10.1104/pp.60.1.64. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kan K. S., Thornber J. P. The Light-harvesting Chlorophyll a/b-Protein Complex of Chlamydomonas reinhardii. Plant Physiol. 1976 Jan;57(1):47–52. doi: 10.1104/pp.57.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Klein S. M., Vernon L. P. Composition of a photosystem I chlorophyll protein complex from Anabaena flos-aquae. Biochim Biophys Acta. 1977 Mar 11;459(3):364–375. doi: 10.1016/0005-2728(77)90038-x. [DOI] [PubMed] [Google Scholar]
  14. Klein S. M., Vernon L. P. Polypeptide Composition of Photosynthetic Membranes from Chlamydomonas reinhardi and Anabaena variabilis. Plant Physiol. 1974 May;53(5):777–778. doi: 10.1104/pp.53.5.777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kung S. D., Thornber J. P. Photosystem I and II chlorophyll-protein complexes of higher plant chloroplasts. Biochim Biophys Acta. 1971 Nov 2;253(1):285–289. doi: 10.1016/0005-2728(71)90255-6. [DOI] [PubMed] [Google Scholar]
  16. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  17. Markwell J. P., Reinman S., Thornber J. P. Chlorophyll-protein complexes from higher plants: a procedure for improved stability and fractionation. Arch Biochem Biophys. 1978 Sep;190(1):136–141. doi: 10.1016/0003-9861(78)90260-6. [DOI] [PubMed] [Google Scholar]
  18. Newman P. J., Sherman L. A. Isolation and characterization of photosystem I and II membrane particles from the blue-green alga, Synechococcus cedrorum. Biochim Biophys Acta. 1978 Aug 8;503(2):343–361. doi: 10.1016/0005-2728(78)90193-7. [DOI] [PubMed] [Google Scholar]
  19. Remy R., Hoarau J., Leclerc J. C. Electrophoretic and spectrophotometric studies of chlorophyll-protein complexes from tobacco chloroplasts. Isolation of a light harvesting pigment protein complex with a molecular weight of 70,000. Photochem Photobiol. 1977 Aug;26(2):151–158. doi: 10.1111/j.1751-1097.1977.tb07466.x. [DOI] [PubMed] [Google Scholar]
  20. Reynolds J. A., Tanford C. The gross conformation of protein-sodium dodecyl sulfate complexes. J Biol Chem. 1970 Oct 10;245(19):5161–5165. [PubMed] [Google Scholar]
  21. Shiozawa J. A., Alberte R. S., Thornber J. P. The P700-chlorophyll a-protein. Isolation and some characteristics of the complex in higher plants. Arch Biochem Biophys. 1974 Nov;165(1):388–397. doi: 10.1016/0003-9861(74)90177-5. [DOI] [PubMed] [Google Scholar]
  22. Thornber J. P., Alberte R. S., Hunter F. A., Shiozawa J. A., Kan K. S. The organization of chlorophyll in the plant photosynthetic unit. Brookhaven Symp Biol. 1976 Jun 7;(28):132–148. [PubMed] [Google Scholar]
  23. Thornber J. P. Comparison of a chlorophyll a- protein complex isolated from a blue-green alga with chlorophyll-protein complexes obtained from green bacteria and higher plants. Biochim Biophys Acta. 1969 Feb 25;172(2):230–241. doi: 10.1016/0005-2728(69)90066-8. [DOI] [PubMed] [Google Scholar]
  24. Wessels J. S., Borchert M. T. Polypeptide profiles of chlorophyll . protein complexes and thylakoid membranes of spinach chloroplasts. Biochim Biophys Acta. 1978 Jul 6;503(1):78–93. doi: 10.1016/0005-2728(78)90163-9. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES