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. 1980 Mar;65(3):415–421. doi: 10.1104/pp.65.3.415

Concentration and Metabolic Turnover of Indoles in Germinating Kernels of Zea mays L. 1

Ephraim Epstein 1,2, Jerry D Cohen 1, Robert S Bandurski 1,3
PMCID: PMC440345  PMID: 16661204

Abstract

The amounts and rates of metabolic turnover of the indolylic compounds in germinating kernels of sweet corn were determined. Knowledge of pool size and rate of pool turnover has permitted: (a) identification of indole-3-acetyl-myo-inositol as the major chemical form for transport of indole-3-acetic acid (IAA) from endosperm to shoot; (b) demonstration that the free IAA of the endosperm is turning over rapidly with a half-life of 3.2 hours; (c) identification of esters of IAA as the immediate precursors of IAA in the endosperm and shoot; (d) demonstration that neither tryptophan nor tryptamine is a major precursor of IAA for the seed or shoot; (e) identification of IAA-myo-inositol glycosides as precursors of IAA-myo-inositol.

It is concluded that seedlings of Zea mays utilize esters of IAA, and not tryptophan or its derivatives, for the IAA requirements of the germinating seedling.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bandurski R. S., Schulze A., Cohen J. D. Photo-regulation of the ratio of ester to free indole-3-acetic acid. Biochem Biophys Res Commun. 1977 Dec 21;79(4):1219–1223. doi: 10.1016/0006-291x(77)91136-6. [DOI] [PubMed] [Google Scholar]
  2. Bandurski R. S., Schulze A. Concentration of Indole-3-acetic Acid and Its Derivatives in Plants. Plant Physiol. 1977 Aug;60(2):211–213. doi: 10.1104/pp.60.2.211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bandurski R. S., Schulze A. Concentrations of Indole-3-acetic Acid and Its Esters in Avena and Zea. Plant Physiol. 1974 Sep;54(3):257–262. doi: 10.1104/pp.54.3.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ehmann A. The van urk-Salkowski reagent--a sensitive and specific chromogenic reagent for silica gel thin-layer chromatographic detection and identification of indole derivatives. J Chromatogr. 1977 Feb 11;132(2):267–276. doi: 10.1016/s0021-9673(00)89300-0. [DOI] [PubMed] [Google Scholar]
  5. Hall P. L., Bandurski R. S. Movement of Indole-3-acetic Acid and Tryptophan-derived Indole-3-acetic Acid from the Endosperm to the Shoot of Zea mays L. Plant Physiol. 1978 Mar;61(3):425–429. doi: 10.1104/pp.61.3.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hamilton R. H. Isolation of indole-3-acetic acid from corn kernels & etiolated corn seedlings. Plant Physiol. 1961 May;36(3):354–359. doi: 10.1104/pp.36.3.354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kopcewicz J., Ehmann A., Bandurski R. S. Enzymatic Esterification of Indole-3-acetic Acid to myo-Inositol and Glucose. Plant Physiol. 1974 Dec;54(6):846–851. doi: 10.1104/pp.54.6.846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Labarca C., Nicholls P. B., Bandurski R. S. A partial characterization of indoleacetylinositols from ZEA mays. Biochem Biophys Res Commun. 1965 Sep 8;20(5):641–646. doi: 10.1016/0006-291x(65)90448-1. [DOI] [PubMed] [Google Scholar]
  9. MONOD J., WYMAN J., CHANGEUX J. P. ON THE NATURE OF ALLOSTERIC TRANSITIONS: A PLAUSIBLE MODEL. J Mol Biol. 1965 May;12:88–118. doi: 10.1016/s0022-2836(65)80285-6. [DOI] [PubMed] [Google Scholar]
  10. Naccarato W. F., Ray R. E., Wells W. W. Characterization and tissue distribution of 6-O-beta-D-galactopyranosyl myo-inositol in the rat. J Biol Chem. 1975 Mar 10;250(5):1872–1876. [PubMed] [Google Scholar]
  11. Nowacki J., Bandurski R. S. Myo-Inositol Esters of Indole-3-acetic Acid as Seed Auxin Precursors of Zea mays L. Plant Physiol. 1980 Mar;65(3):422–427. doi: 10.1104/pp.65.3.422. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Piskornik Z., Bandurski R. S. Purification and Partial Characterization of a Glucan Containing Indole-3-acetic Acid. Plant Physiol. 1972 Jul;50(1):176–182. doi: 10.1104/pp.50.1.176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sherwin J. E., Purves W. K. Tryptophan as an auxin precursor in cucumber seedlings. Plant Physiol. 1969 Sep;44(9):1303–1309. doi: 10.1104/pp.44.9.1303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ueda M., Bandurski R. S. A Quantitative Estimation of Alkali-labile Indole-3-Acetic Acid Compounds in Dormant and Germinating Maize Kernels. Plant Physiol. 1969 Aug;44(8):1175–1181. doi: 10.1104/pp.44.8.1175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ueda M., Ehmann A., Bandurski R. S. Gas-Liquid Chromatographic Analysis of Indole-3-acetic Acid Myoinositol Esters in Maize Kernels. Plant Physiol. 1970 Nov;46(5):715–719. doi: 10.1104/pp.46.5.715. [DOI] [PMC free article] [PubMed] [Google Scholar]

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