Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1980 Apr;65(4):587–590. doi: 10.1104/pp.65.4.587

Adenosine Phosphate and Nicotinamide Adenine Dinucleotide Pool Sizes during Shoot Initiation in Tobacco Callus 1

Daniel C W Brown 1, Trevor A Thorpe 1
PMCID: PMC440387  PMID: 16661243

Abstract

Shoot-forming tobacco callus is found to have high levels of adenosine phosphates and NAD+, and a low energy charge during meristemoid and shoot primordium formation. NADH levels are low and show little change during this period. There is a decline in the content of NADPH to nondetectable levels during the process, and a transient increase of NADP+ is observed early in culture. These patterns are indicative of a shift to a more intensive rate of metabolism during meristemoid and shoot primordium formation and apparently reflect the requirement for energy and reducing power during organ initiation.

Full text

PDF
589

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkinson D. E. Regulation of enzyme function. Annu Rev Microbiol. 1969;23:47–68. doi: 10.1146/annurev.mi.23.100169.000403. [DOI] [PubMed] [Google Scholar]
  2. Bewley J. D., Gwódź E. A. Plant Desiccation and Protein Synthesis: II. On the Relationship between Endogenous Adenosine Triphosphate Levels and Protein-synthesizing Capacity. Plant Physiol. 1975 Jun;55(6):1110–1114. doi: 10.1104/pp.55.6.1110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Day D. A., Wiskich J. T. Pyridine nucleotide interactions with isolated plant mitochondria. Biochim Biophys Acta. 1978 Mar 13;501(3):396–404. doi: 10.1016/0005-2728(78)90107-x. [DOI] [PubMed] [Google Scholar]
  4. FELS I. G. Acid-catalyzed oxidation of reduced pyridine nucleotides. Science. 1961 Jul 28;134(3474):280–281. doi: 10.1126/science.134.3474.280-a. [DOI] [PubMed] [Google Scholar]
  5. GREENBAUM A. L., CLARK J. B., MCLEAN P. THE ESTIMATION OF THE OXIDIZED AND REDUCED FORMS OF THE NICOTINAMIDE NUCLEOTIDES. Biochem J. 1965 Apr;95:161–166. doi: 10.1042/bj0950161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Thorpe T. A., Murashige T. Starch accumulation in shoot-forming tobacco callus cultures. Science. 1968 Apr 26;160(3826):421–422. doi: 10.1126/science.160.3826.421. [DOI] [PubMed] [Google Scholar]
  7. Ting I. P., Dugger W. M., Jr Separation and detection of organic acids on silica gel. Anal Biochem. 1965 Sep;12(3):571–578. doi: 10.1016/0003-2697(65)90224-1. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES