Abstract
Four legume species (four genera) were examined and found to contain hemagglutinins with properties similar to those which we have previously described for the enzymic hemagglutinin in Vigna radiata. Examination of extracts by gel filtration and ion exchange chromatography showed that an α-galactosidase activity exactly co-purified with a hemagglutinin activity in each of the four species. The α-galactosidase activities in the four species were virtually identical to each other with respect to substrate and inhibitor specificity as well as kinetic behavior. Additionally the hemagglutinin activities in all four species displayed very similar carbohydrate specificities. The inhibitor specificities displayed by the enzymes and the hemagglutinins were qualitatively and quantitatively very nearly identical to each other. The remarkable similarities of these proteins, both to each other and to the previously described Vigna enzymic hemagglutinin, suggest that each of these plants may contain a homologue from a specific class of enzymic hemagglutinin.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dey P. M., Pridham J. B. Purification and properties of alpha-galactosidases from Vicia faba seeds. Biochem J. 1969 Jun;113(1):49–55. doi: 10.1042/bj1130049. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Galbraith W., Goldstein I. J. Phytohemagglutinin of the lima bean (Phaseolus lunatus). Isolation, characterization, and interaction with type A blood-group substance. Biochemistry. 1972 Oct 10;11(21):3976–3984. doi: 10.1021/bi00771a022. [DOI] [PubMed] [Google Scholar]
- Hankins C. N., Kindinger J. I., Shannon L. M. Legume Lectins: I. Immunological Cross-Reactions between the Enzymic Lectin from Mung Beans and other Well Characterized Legume Lectins. Plant Physiol. 1979 Jul;64(1):104–107. doi: 10.1104/pp.64.1.104. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hankins C. N., Shannon L. M. The physical and enzymatic properties of a phytohemagglutinin from mung beans. J Biol Chem. 1978 Nov 10;253(21):7791–7797. [PubMed] [Google Scholar]
- Harpaz N., Flowers H. M., Sharon N. alpha-D-galactosidase from soybeans destroying blood-group B antigens. Purification by affinity chromatography and properties. Eur J Biochem. 1977 Jul 15;77(2):419–426. doi: 10.1111/j.1432-1033.1977.tb11682.x. [DOI] [PubMed] [Google Scholar]