Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1980 Apr;65(4):730–734. doi: 10.1104/pp.65.4.730

A Light-dependent Protein Kinase Activity of Chloroplasts 1,2

Ramona Alfonzo 1,3, Nathan Nelson 1,4, Efraim Racker 1
PMCID: PMC440413  PMID: 16661269

Abstract

A protein kinase activity from spinach chloroplasts, tightly associated with the thylakoid membranes, has been solubilized and partially characterized. This membrane-bound protein kinase is stimulated by light and electron transport activity through photosystem II appears to be required for stimulation.

Electron transport inhibitors like 3,4-dichlorophenyl-1,1-dimethylurea, Tris, and NH2OH treatments, inhibit the light activation process. Furthermore, after Tris inhibition, the protein kinase activity is restored by washing the Tris-treated chloroplasts with dichlorophenol indophenol plus ascorbate. The protein kinase remains active in the dark after short illumination periods, suggesting that a product of electron transport may be involved in light activation.

Two endogenous substrates of the protein kinase in thylakoid membranes are the N,N′-dicyclohexylcarbodiimide-reactive proteolipid and the light-harvesting chlorophyll-protein complex. The membrane-bound protein kinase also phosphorylates externally added histone.

Full text

PDF
733

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AVRON M. Photophosphorylation as a tool for the synthesis of specifically labeled nucleotides. Anal Biochem. 1961 Dec;2:535–543. doi: 10.1016/0003-2697(61)90021-5. [DOI] [PubMed] [Google Scholar]
  2. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bensadoun A., Weinstein D. Assay of proteins in the presence of interfering materials. Anal Biochem. 1976 Jan;70(1):241–250. doi: 10.1016/s0003-2697(76)80064-4. [DOI] [PubMed] [Google Scholar]
  4. Burke J. J., Ditto C. L., Arntzen C. J. Involvement of the light-harvesting complex in cation regulation of excitation energy distribution in chloroplasts. Arch Biochem Biophys. 1978 Apr 15;187(1):252–263. doi: 10.1016/0003-9861(78)90031-0. [DOI] [PubMed] [Google Scholar]
  5. Corbin J. D., Reimann E. M. Assay of cyclic AMP-dependent protein kinases. Methods Enzymol. 1974;38:287–290. doi: 10.1016/0076-6879(74)38044-5. [DOI] [PubMed] [Google Scholar]
  6. DE DUVE C., PRESSMAN B. C., GIANETTO R., WATTIAUX R., APPELMANS F. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue. Biochem J. 1955 Aug;60(4):604–617. doi: 10.1042/bj0600604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kamienietzky A., Nelson N. Preparation and properties of chloroplasts depleted of chloroplast coupling factor 1 by sodium bromide treatment. Plant Physiol. 1975 Feb;55(2):282–287. doi: 10.1104/pp.55.2.282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Keates R. A. Cyclic nucleotide-independent protein kinase from pea shoots. Biochem Biophys Res Commun. 1973 Sep 18;54(2):655–661. doi: 10.1016/0006-291x(73)91473-3. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Nakaya N., Sugano N., Nishi A., Tsukada K. Protein kinase in cultured plant cells. Biochim Biophys Acta. 1975 Dec 18;410(2):273–278. doi: 10.1016/0005-2744(75)90229-6. [DOI] [PubMed] [Google Scholar]
  11. Nelson N., Eytan E., Notsani B. E., Sigrist H., Sigrist-Nelson K., Gitler C. Isolation of a chloroplast N,N'-dicyclohexylcarbodiimide-binding proteolipid, active in proton translocation. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2375–2378. doi: 10.1073/pnas.74.6.2375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ort D. R., Izawa S. Studies on the Energy-coupling Sites of Photophosphorylation: II. Treatment of Chloroplasts with NH(2)OH Plus Ethylenediaminetetraacetate to Inhibit Water Oxidation while Maintaining Energy-coupling Efficiencies. Plant Physiol. 1973 Dec;52(6):595–600. doi: 10.1104/pp.52.6.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pick U., Racker E. Purification and reconstitution of the N,N'-dicyclohexylcarbodiimide-sensitive ATPase complex from spinach chloroplasts. J Biol Chem. 1979 Apr 25;254(8):2793–2799. [PubMed] [Google Scholar]
  14. Ralph R. K., McCombs P. J., Tener G., Wojcik S. J. Evidence for modification of protein phosphorylation by cytokinins. Biochem J. 1972 Dec;130(4):901–911. doi: 10.1042/bj1300901a. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rubin C. S., Rosen O. M. Protein phosphorylation. Annu Rev Biochem. 1975;44:831–887. doi: 10.1146/annurev.bi.44.070175.004151. [DOI] [PubMed] [Google Scholar]
  16. Verdanis A. Protein kinase activity at the inner membrane of mammalian mitochondria. J Biol Chem. 1977 Feb 10;252(3):807–813. [PubMed] [Google Scholar]
  17. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES