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. 1980 Apr;65(4):735–745. doi: 10.1104/pp.65.4.735

Flash Inactivation of Oxygen Evolution

IDENTIFICATION OF S2 AS THE TARGET OF INACTIVATION BY TRIS 1,2

Wayne D Frasch 1,3, George M Cheniae 1,4
PMCID: PMC440414  PMID: 16661270

Abstract

Brief saturating light flashes were used to probe the mechanism of inactivation of O2 evolution by Tris in chloroplasts. Maximum inactivation with a single flash and an oscillation with period of four on subsequent flashes was observed. Analyses of the oscillations suggested that only the charge-collecting O2-evolving catalyst of photosystem II (S2-state) was a target of inactivation by Tris. This conclusion was supported by the following observations: (a) hydroxylamine preequilibration caused a three-flash delay in the inactivation pattern; (b) the lifetimes of the Tris-inactivable and S2-states were similar; and (c) reagents accelerating S2 deactivation decreased the lifetime of the inactivable state. Inactivation proved to be moderated by F, the precursor of Signal IIs, as shown by a one flash delay with chloroplasts having high abundance of F. Evidence was obtained for cooperativity effects in inactivation and NH3 was shown to be a competitive inhibitor of the Tris-induced inactivation. S2-dependent inactivation was inhibited by glutaraldehyde fixation of chloroplasts, possibly suggesting that inactivation proceeds via conformational changes of the S2-state.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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