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. 1980 Aug;66(2):291–294. doi: 10.1104/pp.66.2.291

Preparation of Chloroplasts from Euglena Highly Active in Protein Synthesis 1

William Ortiz 2,3, Ellen M Reardon 2, Carl A Price 2
PMCID: PMC440585  PMID: 16661424

Abstract

Chloroplasts can be obtained by gentle lysis or mild shear of spheroplasts of vitamin B12-deficient Euglena gracilis and then purified by isopycnic sedimentation on gradients of Ludox AM or Percoll. The chloroplasts appear compact and highly refractile by phase contrast or Hoffmann contrast microscopy. Upon incubation with [3H]leucine or [35S]methionine, the chloroplasts incorporate the amino acids into protein at rates that are 100-fold faster than we had previously observed with Euglena and up to 8-fold faster than with chloroplasts of spinach. Euglena chloroplasts prepared by the current procedure are thus qualitatively superior to those previously available from Euglena and at least as active in protein synthesis as chloroplasts from higher plants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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