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. 1980 Sep;66(3):400–403. doi: 10.1104/pp.66.3.400

Isolation of Cytoplasmic Enzymes from Pollen 1

Norman F Weeden 1, Leslie D Gottlieb 1
PMCID: PMC440642  PMID: 16661444

Abstract

The cytoplasmic isozyme of many cytoplasmic-organelle isozyme pairs, as well as other cytoplasmic enzymes in plants, can be readily obtained from pollen by soaking it in an appropriate buffer for 4 hours. Enzymes localized in subcellular organelles appear not to be released during the soaking period, although they are released if the pollen is crushed. The technique is a useful initial step in studies of subcellular localization of enzymes or for obtaining small quantities of cytoplasmic enzymes free of organellar contaminants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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