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. 1980 Nov;66(5):847–852. doi: 10.1104/pp.66.5.847

A New Bacterial Agglutinin from Soybean

I. ISOLATION, PARTIAL PURIFICATION, AND CHARACTERIZATION 1

William Frederick Fett 1, Luis Sequeira 1
PMCID: PMC440740  PMID: 16661540

Abstract

A new bacterial agglutinin was isolated from seeds of the soybean cultivar Clark. Purification was carried out by ammonium sulfate precipitation and ion-exchange chromatography. The agglutinin is a heat-labile glycoprotein most active at pH 4.0. Addition of Ca2+, Mn2+ and Mg2+ did not enhance the agglutinating activity of this glycoprotein. Gel electrophoresis in the presence of sodium dodecyl sulfate showed that the agglutinin is composed of two subunits of approximately 50,000 daltons each. In the undissociated state, it agglutinates Xanthomonas phaseoli var. sojensis, the causal agent of bacterial pustule disease of soybean, at concentrations as low as 10 micrograms protein per milliliter but has no hemagglutinating activity. The agglutinin could be distinguished from previously reported soybean lectins on the basis of solubility in ammonium sulfate, lack of hemagglutinating activity, molecular weight, hapten specificity, and immunological determinants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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