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. 1994 Jun 21;91(13):5927–5931. doi: 10.1073/pnas.91.13.5927

Inactivation of the Podospora anserina vegetative incompatibility locus het-c, whose product resembles a glycolipid transfer protein, drastically impairs ascospore production.

S Saupe 1, C Descamps 1, B Turcq 1, J Bégueret 1
PMCID: PMC44110  PMID: 8016091

Abstract

The het-c locus contains different alleles that elicit nonallelic vegetative incompatibility through specific interactions with alleles of the unlinked loci het-e and het-d. The het-c2 allele has been cloned. It encodes a 208-amino acid polypeptide that is similar to a glycolipid transfer protein purified from pig brain. Disruption of this gene drastically impairs ascospore production in homozygous crosses, and some mutants exhibit abnormal branching of apical hyphae. The protein encoded by het-c2 is essential in the biology of the fungus and may be involved in cell-wall biosynthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe A. Primary structure of glycolipid transfer protein from pig brain. J Biol Chem. 1990 Jun 15;265(17):9634–9637. [PubMed] [Google Scholar]
  2. Aitken J. F., van Heusden G. P., Temkin M., Dowhan W. The gene encoding the phosphatidylinositol transfer protein is essential for cell growth. J Biol Chem. 1990 Mar 15;265(8):4711–4717. [PubMed] [Google Scholar]
  3. Akins R. A., Lambowitz A. M. General method for cloning Neurospora crassa nuclear genes by complementation of mutants. Mol Cell Biol. 1985 Sep;5(9):2272–2278. doi: 10.1128/mcb.5.9.2272. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. doi: 10.1016/S0022-2836(05)80360-2. [DOI] [PubMed] [Google Scholar]
  5. BEISSON-SCHECROUN J. [Cellular incompatibility and nucleo-cytoplasmic interactions in the "barrage" phenomena in Podospora anserina]. Ann Genet. 1962;4:4–50. [PubMed] [Google Scholar]
  6. Ballance D. J. Sequences important for gene expression in filamentous fungi. Yeast. 1986 Dec;2(4):229–236. doi: 10.1002/yea.320020404. [DOI] [PubMed] [Google Scholar]
  7. Bergès T., Barreau C. Heat shock at an elevated temperature improves transformation efficiency of protoplasts from Podospora anserina. J Gen Microbiol. 1989 Mar;135(3):601–604. doi: 10.1099/00221287-135-3-601. [DOI] [PubMed] [Google Scholar]
  8. Bernet J. Les systèmes D'incompatibilité chez LE Podospora anserina. C R Acad Sci Hebd Seances Acad Sci D. 1967 Oct 30;265(18):1330–1333. [PubMed] [Google Scholar]
  9. Cleves A., McGee T., Bankaitis V. Phospholipid transfer proteins: a biological debut. Trends Cell Biol. 1991 Jul;1(1):30–34. doi: 10.1016/0962-8924(91)90067-j. [DOI] [PubMed] [Google Scholar]
  10. Delettre Y. M., Bernet J. Regulation of proteolytic enzymes in Podospora anserina: selection and properties of self-lysing mutant strains. Mol Gen Genet. 1976 Mar 22;144(2):191–197. doi: 10.1007/BF02428108. [DOI] [PubMed] [Google Scholar]
  11. Deleu C., Clavé C., Bégueret J. A single amino acid difference is sufficient to elicit vegetative incompatibility in the fungus Podospora anserina. Genetics. 1993 Sep;135(1):45–52. doi: 10.1093/genetics/135.1.45. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Glass N. L., Grotelueschen J., Metzenberg R. L. Neurospora crassa A mating-type region. Proc Natl Acad Sci U S A. 1990 Jul;87(13):4912–4916. doi: 10.1073/pnas.87.13.4912. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hay J. C., Martin T. F. Phosphatidylinositol transfer protein required for ATP-dependent priming of Ca(2+)-activated secretion. Nature. 1993 Dec 9;366(6455):572–575. doi: 10.1038/366572a0. [DOI] [PubMed] [Google Scholar]
  14. Mishra N. C. Genetics and biochemistry of morphogenesis in Neurospora. Adv Genet. 1977;19:341–405. doi: 10.1016/s0065-2660(08)60248-5. [DOI] [PubMed] [Google Scholar]
  15. Perkins A. C., Joshua D. E., Gibson J., Kronenberg H. Fulminant postsplenectomy sepsis. Med J Aust. 1988 Jan 4;148(1):44–46. doi: 10.5694/j.1326-5377.1988.tb104485.x. [DOI] [PubMed] [Google Scholar]
  16. Razanamparany V., Bégueret J. Positive screening and transformation of ura5 mutants in the fungus Podospora anserina: characterization of the transformants. Curr Genet. 1986;10(11):811–817. doi: 10.1007/BF00418527. [DOI] [PubMed] [Google Scholar]
  17. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Smith D. B., Johnson K. S. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene. 1988 Jul 15;67(1):31–40. doi: 10.1016/0378-1119(88)90005-4. [DOI] [PubMed] [Google Scholar]
  19. Staben C., Yanofsky C. Neurospora crassa a mating-type region. Proc Natl Acad Sci U S A. 1990 Jul;87(13):4917–4921. doi: 10.1073/pnas.87.13.4917. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Thomas G. M., Cunningham E., Fensome A., Ball A., Totty N. F., Truong O., Hsuan J. J., Cockcroft S. An essential role for phosphatidylinositol transfer protein in phospholipase C-mediated inositol lipid signaling. Cell. 1993 Sep 10;74(5):919–928. doi: 10.1016/0092-8674(93)90471-2. [DOI] [PubMed] [Google Scholar]
  21. Turcq B., Bégueret J. The ura5 gene of the filamentous fungus Podospora anserina: nucleotide sequence and expression in transformed strains. Gene. 1987;53(2-3):201–209. doi: 10.1016/0378-1119(87)90008-4. [DOI] [PubMed] [Google Scholar]
  22. Turcq B., Deleu C., Denayrolles M., Bégueret J. Two allelic genes responsible for vegetative incompatibility in the fungus Podospora anserina are not essential for cell viability. Mol Gen Genet. 1991 Aug;228(1-2):265–269. doi: 10.1007/BF00282475. [DOI] [PubMed] [Google Scholar]
  23. Wirtz K. W. Phospholipid transfer proteins. Annu Rev Biochem. 1991;60:73–99. doi: 10.1146/annurev.bi.60.070191.000445. [DOI] [PubMed] [Google Scholar]
  24. Yamada K., Abe A., Sasaki T. Specificity of the glycolipid transfer protein from pig brain. J Biol Chem. 1985 Apr 25;260(8):4615–4621. [PubMed] [Google Scholar]

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